公开(公告)号：US20230323327A1

公开(公告)日：2023-10-12

申请号：US18026807

申请日：2021-09-02

Applicant: BASF SE

Inventor： Asfia Qureshi ,  Priya Anand ,  Jared Dennis ,  Hugo Urbina ,  Gaetano Speciale

IPC: C12N9/28

CPC classification number: C12N9/2417

Abstract: The present invention relates to variants of an alpha-amylase which have an increased solubility at pH 6.0, an increased solubility at pH 10.0, a higher resistance to protein aging, and/or an increased specific activity compared to the parent alpha-amylase. The present invention also relates to methods of making the variant alpha-amylase and the use of the variant alpha-amylase in processing starch, cleaning or washing textiles, hard surfaces, or dishes, making ethanol, treating an oil well, processing pulp or paper, animal feed, syrup production, and preparing a dough or a baked product prepared from the dough.

公开(公告)号：US20220186200A1

公开(公告)日：2022-06-16

申请号：US17438088

申请日：2020-03-10

Applicant: BASF SE

Inventor： Hugo Urbina ,  Kristine W. Chang ,  Tong Li ,  Xuqiu Tan ,  Jared Dennis ,  Jochen Kutscher ,  Adrienne Huston Davenport

IPC: C12N9/26

Abstract: Variant polypeptides having G4-amylase activity and methods of making and using the enzymes in baking, detergents, personal care products, in the processing of textiles, in pulp and paper processing, in the production of ethanol, lignocellulosic ethanol, or syrups; or as viscosity breakers in oilfield and mining industries.

公开(公告)号：US20230212544A1

公开(公告)日：2023-07-06

申请号：US18086034

申请日：2022-12-21

Applicant: BASF SE

Inventor： Hugo Urbina ,  Tong Li ,  Jared Dennis ,  Xuqiu Tan ,  Adrienne Huston Davenport ,  Jochen Kutscher ,  Kristine W. Chang

IPC: C12N9/26

CPC classification number: C12N9/2425 ,  C12Y302/01002

Abstract: Variant polypeptides having beta-amylase activity and methods of making and using the enzymes in baking, detergents, personal care products, in the processing of textiles, in pulp and paper processing, in the production of ethanol, lignocellulosic ethanol, or syrups; as viscosity breakers in oilfield and mining industries.

公开(公告)号：US11542487B2

公开(公告)日：2023-01-03

申请号：US16757993

申请日：2018-10-17

Applicant: BASF SE

Inventor： Hugo Urbina ,  Tong Li ,  Jared Dennis ,  Xuqiu Tan ,  Adrienne Huston Davenport ,  Jochen Kutscher ,  Kristine W. Chang

IPC: C12N9/26

Abstract: Variant polypeptides having beta-amylase activity and methods of making and using the enzymes in baking, detergents, personal care products, in the processing of textiles, in pulp and paper processing, in the production of ethanol, lignocellulosic ethanol, or syrups; as viscosity breakers in oilfield and mining industries.

公开(公告)号：US20220162576A1

公开(公告)日：2022-05-26

申请号：US17442489

申请日：2020-03-24

Applicant: BASF SE

Inventor： Stefan Jenewein ,  Zachary David Miles ,  Asfia Qureshi ,  Jared Dennis ,  Tong Li ,  Xuqiu Tan ,  Priya Anand ,  Jesper Nielsen

IPC: C12N9/28

Abstract: Genetically engineered enzymes having amylase enzyme activity, compositions comprising the enzymes, and methods of making and using the enzymes. Fragments of parent amylase enzymes activity and methods of using the fragments for making genetically engineered enzymes having amylase activity. The genetically engineered amylase enzymes are useful in many different applications such as laundry detergents, dish washing detergents, and cleaning products for homes, industry, vehicle care, baking, animal feed, pulp and paper processing, starch processing, brewing, and ethanol production.

公开(公告)号：US12110489B2

公开(公告)日：2024-10-08

申请号：US16954092

申请日：2018-12-18

Applicant: BASF SE

Inventor： Jared Dennis ,  Xuqiu Tan ,  Robin Cai

IPC: C12N15/10 ,  C12N1/20 ,  C12N15/70

CPC classification number: C12N15/1031 ,  C12N1/20 ,  C12N15/70 ,  C12N2800/101

Abstract: Disclosed herein is an improved Gene Site Saturation Mutagenesis (GSSM) method for producing a plurality of modified polynucleotides and/or polypeptides, creating specific changes to a gene, and reassembling mutations or changes at one or more sites.

公开(公告)号：US11634698B2

公开(公告)日：2023-04-25

申请号：US16954276

申请日：2018-12-10

Applicant: BASF SE

Inventor： Xuqiu Tan ,  Kenneth Barrett ,  Jared Dennis ,  Dongmei Ren ,  Corey Dodge ,  Bin Zhang

IPC: C12N9/22 ,  A23K20/147 ,  A23K20/189 ,  A23L29/00 ,  A23L33/18 ,  C11D3/386

Abstract: Disclosed herein are polypeptides having nuclease activity. Some of the polypeptides having nuclease activity were generated by an improved gene site mutagenesis (“GSSM”) method or the tailored multi-site combinatorial assembly (“TMCA”) method. Also disclosed are compositions and kits comprising the polypeptides having nuclease activity, and methods for making and using these polypeptides, compositions and kits.

公开(公告)号：US20220170001A1

公开(公告)日：2022-06-02

申请号：US17442675

申请日：2020-03-24

Applicant: BASF SE

Inventor： Asfia Qureshi ,  Zachary David Miles ,  Xuqiu Tan ,  Tong Li ,  Jared Dennis ,  Stefan Jenewein ,  Priya Anand ,  Jesper Nielsen

IPC: C12N9/26 ,  C12N1/20 ,  C12N15/75 ,  A21D8/04

Abstract: Genetically engineered enzymes having amylase enzyme activity, compositions comprising the enzymes, and methods of making and using the enzymes. Fragments of parent amylase enzymes activity and methods of using the fragments for making genetically engineered enzymes having amylase activity. The genetically engineered amylase enzymes are useful in many different applications such as laundry detergents, dish washing detergents, and cleaning products for homes, industry, vehicle care, baking, animal feed, pulp and paper processing, starch processing, brewing, and ethanol production.