公开(公告)号：US11596876B2

公开(公告)日：2023-03-07

申请号：US16267579

申请日：2019-02-05

Applicant: CLEMSON UNIVERSITY RESEARCH FOUNDATION

Inventor： R. Kenneth Marcus ,  Terri F. Bruce ,  Lei Wang ,  Sisi Huang ,  Tyler Y. Slonecki ,  Rhonda Reigers Powell

IPC: B01D15/32 ,  G01N30/00 ,  C07K14/47 ,  C12N9/00 ,  C12N15/10 ,  G01N1/40 ,  G01N33/50 ,  A61K35/68 ,  B01J20/26 ,  B01J20/28 ,  B01J20/285 ,  B01J20/286

Abstract: A relatively fast, inexpensive, and non-destructive method for separation and isolation of biologically active nanoparticles is described. Methods include the use of solid phase separation medis such as channeled fibers in a hydrophobic interaction chromatography (HIC) protocol to isolate biologically active nanoparticles from other components of a mixture. Biologically active nanoparticles can include natural nanoparticles (e.g., exosomes, lysosomes, virus particles) as well as synthetic nanoparticles (liposomes, genetically modified virus particles, etc.)