公开(公告)号：US20060249687A1

公开(公告)日：2006-11-09

申请号：US11216049

申请日：2005-09-01

Applicant: Chih-Yu Chao ,  Wen-Jiunn Hsieh

Inventor： Chih-Yu Chao ,  Wen-Jiunn Hsieh

IPC: H01J37/18

CPC classification number: H01J37/20 ,  H01J2237/2002

Abstract: A device for operating gas in the vacuum or low-pressure environment and for observation of the operation includes a housing. The housing has a thinner part formed at a side thereof, and at least one spacer mounted therein for partitioning off its inside into a gas chamber and at least one buffer chamber outside the gas chamber. The gas chamber has two inner apertures provided on the spacers above and below the gas chamber. The housing has two outer apertures provided respectively on a top side thereof and a bottom side thereof. All of the inner and outer apertures are coaxial with one another and located on the thinner part. The housing has a pumping port for communication with the buffer chamber, and a gas inlet for communication with the gas chamber.

公开(公告)号：US20090242795A1

公开(公告)日：2009-10-01

申请号：US12078223

申请日：2008-03-28

Applicant: Chih-Yu Chao

Inventor： Chih-Yu Chao

IPC: H01J37/20 ,  G21K5/10

CPC classification number: H01J37/20 ,  G01N1/42 ,  H01J2237/2001 ,  H01J2237/2002 ,  H01J2237/2003 ,  H01J2237/2004 ,  H01J2237/26

Abstract: The present invention relates to a cryo-charging specimen holder for the electron microscope, particularly to a cryo-charging specimen holder for the electron microscope to hold various biological materials. The major feature of the invention is to charge the biological specimen and freeze the specimen at low temperature. The ice around the biological sample is also doped, so that after charging the doped ice surrounding the sample has a conductivity level comparable to that of conductor. Therefore, the sample can be embedded by the doped and charged ice obtaining the property of conductor, in order to be observed by the electron microscope.

Abstract translation: 本发明涉及一种用于电子显微镜的低温充电试样架，特别涉及用于电子显微镜保持各种生物材料的低温充电试样架。 本发明的主要特征是对生物样品充电并在低温下冷冻试样。 生物样品周围的冰也被掺杂，因此在充电之后，样品周围的掺杂冰具有与导体相当的导电率。 因此，可以通过掺杂和充电的冰来嵌入样品，获得导体的性质，以便通过电子显微镜观察。

公开(公告)号：US07544954B2

公开(公告)日：2009-06-09

申请号：US11429153

申请日：2006-05-08

Applicant: Chih-Yu Chao ,  Wen-Jiunn Hsieh

Inventor： Chih-Yu Chao ,  Wen-Jiunn Hsieh

IPC: G01F23/00

CPC classification number: H01J37/20 ,  H01J37/26 ,  H01J2237/2003

Abstract: A device for operating gas in the vacuum or low-pressure environment and for observation of the operation includes a housing. The housing has a thinner part formed at a side thereof, and at least one spacer mounted therein for partitioning off its inside into a gas chamber and at least one buffer chamber outside the gas chamber. The gas chamber has two inner apertures provided on the spacers above and below the gas chamber. The housing has two outer apertures provided respectively on a top side thereof and a bottom side thereof. All of the inner and outer apertures are coaxial with one another and located on the thinner part. The housing has a pumping port for communication with the buffer chamber, and a gas inlet for communication with the gas chamber.

Abstract translation: 用于在真空或低压环境中操作气体并用于观察操作的装置包括壳体。 壳体的一侧形成有较薄的部分，并且至少一个间隔件安装在其中以将其内部分隔成气体室和在气体室外的至少一个缓冲室。 气室具有设置在气室上方和下方的间隔壁上的两个内孔。 壳体具有分别设置在其顶侧和底侧上的两个外孔。 所有内孔和外孔彼此同轴并位于较薄部分上。 壳体具有用于与缓冲室连通的泵送口和用于与气室连通的气体入口。

公开(公告)号：US07432511B2

公开(公告)日：2008-10-07

申请号：US11216044

申请日：2005-09-01

Applicant: Chih-Yu Chao ,  Wen-Jiunn Hsieh

Inventor： Chih-Yu Chao ,  Wen-Jiunn Hsieh

IPC: G01F23/00 ,  G21K5/08 ,  G21K5/10

CPC classification number: H01J37/26 ,  H01J37/20 ,  H01J2237/2003

Abstract: A method of operating liquid in a vacuum or low-pressure environment and observing the operation and a device for the operation and the observation respectively, including the steps of preparing a housing, putting the housing in the vacuum or low-pressure environment and control liquid, vapor, and buffer chambers under the same temperature, infusing vapor into the vapor chamber through a gas inlet and control the vapor pressure inside the vapor chamber to be equal to the saturated vapor pressure of a liquid specimen inside the liquid chamber under the same temperature to prevent the inside liquid from volatilization, and evacuating the buffer chamber through the pumping port to pump out the vapor and prevent the vapor from exhausting through outer apertures out of the housing. A probing source can pass through the outer, inner, and vapor apertures for observation and analysis of the liquid specimen inside the liquid chamber.

Abstract translation: 一种在真空或低压环境中操作液体的方法，分别观察操作和操作和观察装置，包括准备壳体，将壳体放置在真空或低压环境中的步骤和控制液体 ，蒸汽和缓冲室，通过气体入口将蒸汽输入蒸气室，并将蒸汽室内的蒸汽压力控制在相同温度下等于液体室内液体试样的饱和蒸气压 以防止内部液体挥发，并且通过泵送端口排出缓冲室以泵出蒸汽并防止蒸气通过外部孔排出外壳。 探测源可以通过外部，内部和蒸气孔径，用于观察和分析液体室内的液体试样。

公开(公告)号：US20080073532A1

公开(公告)日：2008-03-27

申请号：US11637769

申请日：2006-12-13

Applicant: Chih-Yu Chao ,  Wen-Jiunn Hsieh

Inventor： Chih-Yu Chao ,  Wen-Jiunn Hsieh

IPC: G21K7/00

CPC classification number: H01J37/26 ,  H01J37/18 ,  H01J2237/006 ,  H01J2237/1405 ,  H01J2237/188 ,  H01J2237/2608

Abstract: An observational liquid/gas environment combined with a specimen chamber and two pole pieces of an electron microscope includes at least two buffer chambers, a plurality of spacers, and a gas source. The buffer chambers are formed by the spacers and the two pole pieces, located at an upper side and a lower side of the specimen chamber respectively. The spacers have inner and outer apertures abutting the buffer chambers. All of the inner and outer apertures are coaxially aligned with one another, crossing a path that the electron beam of the electron microscope passes. The buffer chambers are connected with a gas-pumping source. The gas source is connected with the specimen chamber. The distance between the at least two inner apertures is smaller than that of the two pole pieces. The spacers having the inner apertures are located in the specimen chamber or the electron beam through tunnels.

Abstract translation: 与样品室结合的观察性液体/气体环境和电子显微镜的两个极片包括至少两个缓冲室，多个间隔物和气体源。 缓冲室由分隔件和两个极片分别形成，分别位于试样室的上侧和下侧。 间隔物具有邻接缓冲室的内孔和外孔。 所有内孔和外孔彼此同轴对准，与电子显微镜的电子束通过的路径相交。 缓冲室与气体泵送源连接。 气源与样品室连接。 至少两个内孔之间的距离小于两个极片的距离。 具有内孔的间隔物位于试样室中或电子束通过隧道。

公开(公告)号：US20060249677A1

公开(公告)日：2006-11-09

申请号：US11429153

申请日：2006-05-08

Applicant: Chih-Yu Chao ,  Wen-Jiunn Hsieh

Inventor： Chih-Yu Chao ,  Wen-Jiunn Hsieh

IPC: G21K7/00

CPC classification number: H01J37/20 ,  H01J37/26 ,  H01J2237/2003

Abstract: A device for operating gas in the vacuum or low-pressure environment and for observation of the operation includes a housing. The housing has a thinner part formed at a side thereof, and at least one spacer mounted therein for partitioning off its inside into a gas chamber and at least one buffer chamber outside the gas chamber. The gas chamber has two inner apertures provided on the spacers above and below the gas chamber. The housing has two outer apertures provided respectively on a top side thereof and a bottom side thereof. All of the inner and outer apertures are coaxial with one another and located on the thinner part. The housing has a pumping port for communication with the buffer chamber, and a gas inlet for communication with the gas chamber.

公开(公告)号：US07554099B2

公开(公告)日：2009-06-30

申请号：US11483765

申请日：2006-07-11

Applicant: Chih-Yu Chao ,  Wen-Jiunn Hsieh

Inventor： Chih-Yu Chao ,  Wen-Jiunn Hsieh

IPC: G01F23/00 ,  G21K5/08 ,  G21K5/10 ,  G01N23/00 ,  G21K7/00

CPC classification number: H01J37/20 ,  H01J2237/2003 ,  H01J2237/2608

Abstract: An ultra-thin liquid control plate and a combination of a box-like member and the control plate include a plate-like member having at least one view hole. The joint surface combined with a sidewall of the view hole and a surface of the plate-like member is provided with at least one more-hydrophilic section and at least one less-hydrophilic section, wherein the more-hydrophilic section has a height smaller than 50 μm. While combined with the box-like member, the plate-like member is mounted in the box-like member. The box-like member has at least one through hole running through each of a top side and a bottom side thereof and coaxially aligned with the view hole. After a liquid is placed into the view hole, the more-hydrophilic section adsorbs the liquid to form a liquid layer and the less-hydrophilic section is hydrophobic to adsorb no liquid.

Abstract translation: 超薄液体控制板和盒状部件与控制板的组合包括具有至少一个视野孔的板状部件。 与观察孔的侧壁和板状构件的表面结合的接合表面设置有至少一个更亲水部分和至少一个较少亲水部分，其中较亲水部分的高度小于 妈妈妈妈 当与盒状构件组合时，板状构件安装在盒状构件中。 盒状构件具有穿过其顶侧和底侧中的每一个的至少一个通孔，并与观察孔同轴对准。 在将液体放入观察孔中之后，较亲水部分吸附液体以形成液体层，较不亲水的部分是疏水性的，以吸附液体。

公开(公告)号：US20070145289A1

公开(公告)日：2007-06-28

申请号：US11584559

申请日：2006-10-23

Applicant: Chih-Yu Chao ,  Wen-Jiunn Hsieh

Inventor： Chih-Yu Chao ,  Wen-Jiunn Hsieh

IPC: G21K5/10

CPC classification number: H01J37/20 ,  H01J2237/188 ,  H01J2237/2004 ,  H01J2237/262

Abstract: A closed observational device for an electron microscope is formed of a housing. The housing includes a liquid chamber formed therein, at least one view hole formed at each of a top side thereof and a bottom side thereof and communicating with the liquid chamber and coaxially aligned with the other, and a film mounted to and sealing each of the view holes. Accordingly, a general specimen or a live cell can be placed into the liquid chamber for microscopic observation under the electron microscope. Besides, the present invention can enclose the liquid inside the housing to prevent the liquid from exhausting outward or volatilization.

Abstract translation: 用于电子显微镜的闭合观察装置由壳体形成。 壳体包括形成在其中的液体室，形成在其顶侧和底侧的至少一个视野孔，并且与液体室连通并与另一个同轴地对准，并且安装并密封每个 查看孔。 因此，可以在电子显微镜下将一般试样或活细胞置于液室内进行显微镜观察。 此外，本发明可以将液体包围在壳体内，以防止液体向外或挥发中排出。

公开(公告)号：US20070145268A1

公开(公告)日：2007-06-28

申请号：US11483765

申请日：2006-07-11

Applicant: Chih-Yu Chao ,  Wen-Jiunn Hsieh

Inventor： Chih-Yu Chao ,  Wen-Jiunn Hsieh

IPC: G21K7/00

CPC classification number: H01J37/20 ,  H01J2237/2003 ,  H01J2237/2608

Abstract: An ultra-thin liquid control plate and a combination of a box-like member and the control plat include a plate-like member having at least one view hole. The joint surface combined with a sidewall of the view hole and a surface of the plate-like member is provided with at least one more-hydrophilic section and at least one less-hydrophilic section, wherein the more-hydrophilic section has a height smaller than 50 μm. While combined with the box-like member, the plate-like member is mounted in the box-like member. The box-like member has at least one through hole running through each of a top side and a bottom side thereof and coaxially aligned with the view hole. After a liquid is placed into the view hole, the more-hydrophilic section adsorbs the liquid to form a liquid layer and the less-hydrophilic section is hydrophobic to adsorb no liquid.

Abstract translation: 超薄液体控制板和盒状部件与控制台的组合包括具有至少一个视野孔的板状部件。 与观察孔的侧壁和板状构件的表面结合的接合表面设置有至少一个更亲水部分和至少一个较少亲水部分，其中较亲水部分的高度小于 妈妈妈妈 当与盒状构件组合时，板状构件安装在盒状构件中。 盒状构件具有穿过其顶侧和底侧中的每一个的至少一个通孔，并与观察孔同轴对准。 在将液体放入观察孔中之后，较亲水部分吸附液体以形成液体层，较不亲水的部分是疏水性的，以吸附液体。

公开(公告)号：US20060261284A1

公开(公告)日：2006-11-23

申请号：US11216044

申请日：2005-09-01

Applicant: Chih-Yu Chao ,  Wen-Jiunn Hsieh

Inventor： Chih-Yu Chao ,  Wen-Jiunn Hsieh

IPC: G21K5/10 ,  G01F23/00

CPC classification number: H01J37/26 ,  H01J37/20 ,  H01J2237/2003

Abstract: A method of operating liquid in a vacuum or low-pressure environment and observing the operation and a device for the operation and the observation respectively, including the steps of preparing a housing, putting the housing in the vacuum or low-pressure environment and control liquid, vapor, and buffer chambers under the same temperature, infusing vapor into the vapor chamber through a gas inlet and control the vapor pressure inside the vapor chamber to be equal to the saturated vapor pressure of a liquid specimen inside the liquid chamber under the same temperature to prevent the inside liquid from volatilization, and evacuating the buffer chamber through the pumping port to pump out the vapor and prevent the vapor from exhausting through outer apertures out of the housing. A probing source can pass through the outer, inner, and vapor apertures for observation and analysis of the liquid specimen inside the liquid chamber.

Abstract translation: 一种在真空或低压环境中操作液体的方法，分别观察操作和操作和观察装置，包括准备壳体，将壳体放置在真空或低压环境中的步骤和控制液体 ，蒸汽和缓冲室，通过气体入口将蒸汽输入蒸气室，并将蒸汽室内的蒸汽压力控制在相同温度下等于液体室内液体试样的饱和蒸气压 以防止内部液体挥发，并且通过泵送端口排出缓冲室以泵出蒸汽并防止蒸气通过外部孔排出外壳。 探测源可以通过外部，内部和蒸气孔径，用于观察和分析液体室内的液体试样。