公开(公告)号：US20220219141A1

公开(公告)日：2022-07-14

申请号：US17605502

申请日：2020-04-28

Applicant: Cytiva Bioprocess R&D AB

Inventor： Susanna Lindberg ,  Jonas Gustafsson ,  Linn Carlsson ,  Anders Hagvall ,  Erik Ringberg ,  David Jansson

IPC: B01J20/291 ,  B01J20/24 ,  B01J20/28 ,  B01J20/30

Abstract: The invention discloses method for manufacturing agar or agarose beads, comprising the steps of: a) providing a water phase comprising an aqueous solution of agar or agarose at a temperature of 40-100° C.: b) providing an oil phase comprising a water-immiscible solvent and an emulsifier at a temperature of 40-100° C.; c) emulsifying the water phase in the oil phase to form a water-in-oil emulsion: d) cooling the water-in-oil emulsion to a temperature below a gelation temperature of the agar or agarose to form a dispersion of solidified agar or agarose beads: and c) recovering agar or agarose beads from dispersion, wherein the emulsifier comprises a phosphate ester of an alkoxy lated fatty alcohol.

公开(公告)号：US12251681B2

公开(公告)日：2025-03-18

申请号：US17605502

申请日：2020-04-28

Applicant: Cytiva Bioprocess R&D AB

Inventor： Susanna Lindberg ,  Jonas Gustafsson ,  Linn Carlsson ,  Anders Hagvall ,  Erik Ringberg ,  David Jansson

IPC: B01J20/29 ,  B01J20/24 ,  B01J20/28 ,  B01J20/291 ,  B01J20/30

Abstract: The invention discloses method for manufacturing agar or agarose beads, comprising the steps of: a) providing a water phase comprising an aqueous solution of agar or agarose at a temperature of 40-100° C.: b) providing an oil phase comprising a water-immiscible solvent and an emulsifier at a temperature of 40-100° C.; c) emulsifying the water phase in the oil phase to form a water-in-oil emulsion: d) cooling the water-in-oil emulsion to a temperature below a gelation temperature of the agar or agarose to form a dispersion of solidified agar or agarose beads: and e) recovering agar or agarose beads from dispersion, wherein the emulsifier comprises a phosphate ester of an alkoxylated fatty alcohol.

公开(公告)号：US12005424B2

公开(公告)日：2024-06-11

申请号：US17045779

申请日：2019-04-24

Applicant: Cytiva BioProcess R&D AB

Inventor： Jonas Bengtsson ,  Linn Carlsson ,  Andreas Axen ,  Ronnie Palmgren

IPC: B01D15/08 ,  B01J20/24 ,  B01J20/26 ,  B01J20/28 ,  B01J20/285 ,  B01J20/291 ,  B01J20/30

CPC classification number: B01J20/267 ,  B01D15/08 ,  B01J20/24 ,  B01J20/28023 ,  B01J20/28047 ,  B01J20/285 ,  B01J20/291 ,  B01J20/3042 ,  B01J2220/445 ,  B01J2220/54

Abstract: The invention discloses a separation matrix comprising polysaccharide gel beads, wherein said polysaccharide gel beads comprise embedded fibers. The invention further discloses a method of preparing the separation matrix and use of the matrix for separation purposes.

公开(公告)号：US20210060525A1

公开(公告)日：2021-03-04

申请号：US17045779

申请日：2019-04-24

Applicant: Cytiva BioProcess R&D AB

Inventor： Jonas Bengtsson ,  Linn Carlsson ,  Andreas Axen ,  Ronnie Palmgren

IPC: B01J20/24 ,  B01J20/28 ,  B01J20/291 ,  B01J20/30 ,  B01J20/281 ,  B01D15/08

Abstract: The invention discloses a separation matrix comprising polysaccharide gel beads, wherein said polysaccharide gel beads comprise embedded fibers. The invention further discloses a method of preparing the separation matrix and use of the matrix for separation purposes.