公开(公告)号：US11034963B2

公开(公告)日：2021-06-15

申请号：US16347935

申请日：2017-11-08

Applicant: DOTS Technology Corp.

Inventor： Adi Gilboa-Geffen ,  Valerie Villareal ,  Sarah Stidham ,  Morris Nehama

IPC: C12N15/115 ,  C12N15/10 ,  G01N33/02 ,  G01N33/533 ,  C12Q1/6816

Abstract: The present application discloses nucleic acid aptamer based signaling polynucleotides (SPNs) that specifically bind an allergen protein. Provided in the present invention include aptamers, SPNs, SPN-complement complexes, magnetic particle conjugates, DNA printed glass slides and detection agents, and detection methods using the same for detecting the presence, or absence of an allergen protein in a food sample.

公开(公告)号：US20180188139A1

公开(公告)日：2018-07-05

申请号：US15736801

申请日：2016-06-20

Applicant: DOTS Technology Corp.

Inventor： Adi Gilboa-Geffen ,  Renuka Babu Brown

IPC: G01N1/28 ,  G01N33/543 ,  G01N33/53 ,  G01N33/68 ,  G01N1/40 ,  G01N33/02 ,  G01N33/00

CPC classification number: G01N1/286 ,  G01N1/4044 ,  G01N33/0098 ,  G01N33/02 ,  G01N33/025 ,  G01N33/5304 ,  G01N33/54366 ,  G01N33/6854 ,  G01N2001/2866

Abstract: The present invention relates to assays and methods for detecting the presence, absence and/or amount of an allergen (e.g., a food allergen) in a sample. The current assays and methods improve sampling processes, increase detection accuracy and sensitivity, and reduce the detection time.

公开(公告)号：US20210389245A1

公开(公告)日：2021-12-16

申请号：US17282807

申请日：2019-10-04

Applicant: DOTS Technology Corp.

Inventor： Adi Gilboa-Geffen ,  Alan Lloyd Weeks ,  Valerie Villareal ,  Patrick Murphy ,  Eric Anthony Robertson ,  Deirdre Ellen Day ,  Matthew Bernard Dean ,  Todd Glendon Campbell ,  Brian Christopher Burke ,  Tyler S. Smith ,  Thomas Christopher Hartner ,  Stanley Owen Thompson ,  Nhat Nam Trinh ,  David Carpenter ,  Gregory J. Kintz ,  Paul Koh ,  David Jennings Dostal ,  Kevin Doherty ,  Joel F. Jensen ,  William Law ,  Russell C. Mead, Jr. ,  J. Efraín Alcorta

IPC: G01N21/64 ,  B01L3/00

Abstract: The present disclosure is drawn to devices and systems for target detection in samples, particularly allergen detection in food samples. The allergen detection system includes a sampler, a disposable analysis cartridge and a detection device with an optimized optical system. The allergen detection utilizes nucleic acid molecules as detection agents and detection probes.

公开(公告)号：US20210311032A1

公开(公告)日：2021-10-07

申请号：US16971701

申请日：2019-02-21

Applicant: DOTS Technology Corp.

Inventor： Adi Gilboa-Geffen ,  Alan Lloyd Weeks ,  Valerie Villareal ,  Patrick Murphy ,  Eric Anthony Robertson ,  David Carpenter ,  Deirdre Ellen Day ,  Matthew Bernard Dean ,  Todd Glendon Campbell ,  Gregory J. Kintz ,  Paul Koh ,  David Jennings Dostal ,  Kevin Doherty ,  Joel F. Jensen ,  William Law ,  Russell C. Mead, Jr. ,  J. Efraín Alcorta

IPC: G01N33/53 ,  G01N33/543 ,  C12Q1/6816 ,  G01N33/02

Abstract: The present invention is drawn to devices and systems for allergen detection in food samples. The allergen detection system includes a disposable analysis cartridge and a detection device with an optimized optical system.

公开(公告)号：US20210109076A1

公开(公告)日：2021-04-15

申请号：US17128270

申请日：2020-12-21

Applicant: DOTS Technology Corp.

Inventor： Adi Gilboa-Geffen ,  Renuka Babu Brown ,  Sarah Stidham ,  Valerie Villareal ,  Adam J. Young ,  Joshua Glenn Anthony ,  Patrick Murphy ,  John H. Kepler ,  Adam Jacobs

IPC: G01N33/02 ,  C12M1/34 ,  G01N1/08 ,  C12N15/115 ,  G01N1/14 ,  G01N1/28 ,  G01N33/53 ,  G01N33/58

Abstract: The present invention provides systems, devices, signaling polynucleotides (SPNs), detection agents and methods for detecting the presence and/or absence of one or more allergens in a sample particularly a food sample. The detection system includes a separate sampler, at least one disposable detection vessel for receiving and processing a test sample and a detection device for measuring a fluorescent signal. SPNs derived from aptamer that bind allergens are provided as detection agents. SPNs have a single open structure, and are labeled with a fluorophore. Changes in fluorescence polarization of SPNs upon the binding of allergens are measured to calculate the allergen content in a sample.

公开(公告)号：US10466246B2

公开(公告)日：2019-11-05

申请号：US15569808

申请日：2016-04-26

Applicant: DOTS Technology Corp.

Inventor： Adi Gilboa-Geffen

IPC: G01N33/58 ,  G01N33/68 ,  A61K38/00 ,  C12M1/34 ,  G01N33/53 ,  G01N33/542 ,  A61P37/00

Abstract: The present invention is drawn to nucleic acid aptamer based signaling polynucleotides (SPNs) for allergen detection in samples. Disclosed herein include compositions, compounds, assays and methods of using said SPNs to detect one or more allergens in a sample, particularly food allergens in a food product.

公开(公告)号：US20220298570A1

公开(公告)日：2022-09-22

申请号：US17636403

申请日：2020-08-20

Applicant: DOTS Technology Corp.

Inventor： Adi Gilboa-Geffen ,  Valerie Villareal ,  Patrick Murphy ,  Nhat Nam Trinh

IPC: C12Q1/6876 ,  C12Q1/6834

Abstract: The present disclosure relates to optimized nucleic acid probes, nucleic acid chips, and assays and methods for detection of an analyte of interest in a sample, for example, an allergen in a food sample.

公开(公告)号：US11268136B2

公开(公告)日：2022-03-08

申请号：US16419150

申请日：2019-05-22

Applicant: DOTS Technology Corp.

Inventor： Adi Gilboa-Geffen ,  Renuka Babu Brown

IPC: C07H21/02 ,  C07H21/04 ,  C12Q1/6818 ,  G01N33/02 ,  C12Q1/6811 ,  G01N33/53 ,  C12N15/115 ,  C12N15/10

Abstract: The present invention provides allergen detection molecules and devices useful in on-site and rapid detection of allergens, including food allergens.

公开(公告)号：US20190119669A1

公开(公告)日：2019-04-25

申请号：US16093200

申请日：2017-04-11

Applicant: DOTS Technology Corp.

Inventor： Adi Gilboa-Geffen ,  Renuka Babu Brown

IPC: C12N15/10 ,  C12Q1/6811 ,  G01N33/543

Abstract: The present invention relates to assays and methods for target analyte (e.g., allergen) detection in a sample using aptamer-magnetic particle complexes.

公开(公告)号：US20180128835A1

公开(公告)日：2018-05-10

申请号：US15569808

申请日：2016-04-26

Applicant: DOTS Technology Corp.

Inventor： Adi Gilboa-Geffen

IPC: G01N33/58 ,  G01N33/68 ,  C12M1/34 ,  A61K38/00

CPC classification number: G01N33/582 ,  A61K38/00 ,  A61P37/00 ,  C12M1/34 ,  C12N2310/16 ,  G01N33/5308 ,  G01N33/542 ,  G01N33/68 ,  G01N2800/24

Abstract: The present invention is drawn to nucleic acid aptamer based signaling polynucleotides (SPNs) for allergen detection in samples. Disclosed herein include compositions, compounds, assays and methods of using said SPNs to detect one or more allergens in a sample, particularly food allergens in a food product.