公开(公告)号：US20160016164A1

公开(公告)日：2016-01-21

申请号：US14720305

申请日：2015-05-22

Applicant: Fluidigm Corporation

Inventor： David Cohen ,  Andrew May ,  Martin Pieprzyk ,  Kim Huat Lee ,  Jun Yan ,  Ming Fang Zhou ,  Seng Beng Ng

IPC: B01L3/00

CPC classification number: B01L3/5027 ,  B01L3/502738 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/12 ,  B01L2300/045 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0655 ,  G01N1/405

Abstract: The present invention includes microfluidic systems having a microfabricated cavity that may be covered with a removable cover, where the removable cover allows at least part of the opening of the microfabricated cavity to be exposed or directly accessed by an operator. The microfluidic systems comprise chambers, flow and control channels formed in elastomeric layers that may comprise PDMS. The removable cover comprises a thermoplastic base film bonded to an elastomer layer by an adhesive layer. When the removable cover is peeled off, the chamber is at least partially open to allow sample extraction from the chamber. The chamber may have macromolecular crystals formed inside or resulting contents from a PCR reaction. The invention also includes a method for making vias in elastomeric layers by using the removable cover. The invention further includes methods and devices for peeling the peelable cover or a removable component such as Integrated Heater Spreader.

Abstract translation: 本发明包括具有微型空腔的​​微流体系统，其可以被可移除的盖覆盖，其中可移除的盖允许微加工腔的至少一部分开口被操作者暴露或直接访问。 微流体系统包括在可包括PDMS的弹性体层中形成的腔室，流动和控制通道。 可移除的盖包括通过粘合剂层结合到弹性体层的热塑性基底膜。 当可移除的盖被剥离时，室至少部分地打开以允许从室中取样。 该室可能在PCR反应内部形成大分子晶体或产生内含物。 本发明还包括通过使用可移除盖制造弹性体层中的通孔的方法。 本发明还包括用于剥离可剥离盖或诸如集成式加热器撒布机的可移除部件的方法和装置。

公开(公告)号：US20140045184A1

公开(公告)日：2014-02-13

申请号：US13784736

申请日：2013-03-04

Applicant: Fluidigm Corporation

Inventor： Martin Pieprzyk ,  Geoff Facer ,  Timothy Woudenberg ,  Brian Fowler

IPC: C12Q1/68

CPC classification number: G01N1/28 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/16 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  B33Y80/00 ,  C12Q1/686 ,  G01N21/05 ,  G01N21/6452 ,  G01N2021/0346 ,  Y10T137/7793 ,  Y10T436/2575

Abstract: Embodiments of the present invention provide improved microfluidic devices and related apparatus, systems, and methods. Methods are provided for reducing mixing times during use of microfluidic devices. Microfluidic devices and related methods of manufacturing are provided with increased manufacturing yield rates. Improved apparatus and related systems are provided for supplying controlled pressure to microfluidic devices. Methods and related microfluidic devices are provided for reducing dehydration of microfluidic devices during use. Microfluidic devices and related methods are provided with improved sample to reagent mixture ratio control. Microfluidic devices and systems are provided with improved resistance to compression fixture pressure induced failures. Methods and systems for conducting temperature controlled reactions using microfluidic devices are provided that reduce condensation levels within the microfluidic device. Methods and systems are provided for improved fluorescent imaging of microfluidic devices.

Abstract translation: 本发明的实施例提供了改进的微流体装置和相关装置，系统和方法。 提供了减少使用微流体装置期间的混合时间的方法。 微流控装置和相关的制造方法具有提高的制造产率。 提供改进的装置和相关系统用于向微流体装置提供受控的压力。 提供了方法和相关的微流体装置，用于在使用过程中减少微流体装置的脱水。 微流控装置和相关方法提供了改进的样品与试剂混合比控制。 微流体装置和系统具有改进的抗压缩夹具压力引起的故障的阻力。 提供了使用微流体装置进行温度控制反应的方法和系统，其减少微流体装置内的冷凝水平。 提供了用于改进微流体装置的荧光成像的方法和系统。

公开(公告)号：US20150185118A1

公开(公告)日：2015-07-02

申请号：US14477642

申请日：2014-09-04

Applicant: Fluidigm Corporation

Inventor： Martin Pieprzyk ,  Geoff Facer ,  Timothy Woudenberg ,  Brian Fowler

IPC: G01N1/28

CPC classification number: G01N1/28 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/16 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  B33Y80/00 ,  C12Q1/686 ,  G01N21/05 ,  G01N21/6452 ,  G01N2021/0346 ,  Y10T137/7793 ,  Y10T436/2575

Abstract: Embodiments of the present invention provide improved microfluidic devices and related apparatus, systems, and methods. Methods are provided for reducing mixing times during use of microfluidic devices. Microfluidic devices and related methods of manufacturing are provided with increased manufacturing yield rates. Improved apparatus and related systems are provided for supplying controlled pressure to microfluidic devices. Methods and related microfluidic devices are provided for reducing dehydration of microfluidic devices during use. Microfluidic devices and related methods are provided with improved sample to reagent mixture ratio control. Microfluidic devices and systems are provided with improved resistance to compression fixture pressure induced failures. Methods and systems for conducting temperature controlled reactions using microfluidic devices are provided that reduce condensation levels within the microfluidic device. Methods and systems are provided for improved fluorescent imaging of microfluidic devices.

Abstract translation: 本发明的实施例提供了改进的微流体装置和相关装置，系统和方法。 提供了减少使用微流体装置期间的混合时间的方法。 微流控装置和相关的制造方法具有提高的制造产率。 提供改进的装置和相关系统用于向微流体装置提供受控的压力。 提供了方法和相关的微流体装置，用于在使用过程中减少微流体装置的脱水。 微流控装置和相关方法提供了改进的样品与试剂混合比控制。 微流体装置和系统具有改进的抗压缩夹具压力引起的故障的阻力。 提供了使用微流体装置进行温度控制反应的方法和系统，其减少微流体装置内的冷凝水平。 提供了用于改进微流体装置的荧光成像的方法和系统。

公开(公告)号：US09909179B2

公开(公告)日：2018-03-06

申请号：US14977414

申请日：2015-12-21

Applicant: Fluidigm Corporation

Inventor： Amy Hamilton ,  Min Lin ,  Alain Mir ,  Martin Pieprzyk

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6881 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2531/113 ,  C12Q2531/119 ,  C12Q2531/137 ,  C12Q2565/101 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/178

Abstract: The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.

公开(公告)号：US20160340728A1

公开(公告)日：2016-11-24

申请号：US14977414

申请日：2015-12-21

Applicant: Fluidigm Corporation

Inventor： Amy Hamilton ,  Min Lin ,  Alain Mir ,  Martin Pieprzyk

IPC: C12Q1/68

CPC classification number: C12Q1/6881 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2531/113 ,  C12Q2531/119 ,  C12Q2531/137 ,  C12Q2565/101 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/178

Abstract: The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.

Abstract translation: 本发明提供从小样品甚至单细胞分析基因组DNA和/或RNA的方法。 分析基因组DNA的方法可能需要全基因组扩增（WGA），然后对选定的靶核酸进行预扩增和扩增。 分析RNA的方法可能需要所需RNA的逆转录，然后对选定的靶核酸进行预扩增和扩增。

公开(公告)号：US09498776B2

公开(公告)日：2016-11-22

申请号：US14720305

申请日：2015-05-22

Applicant: Fluidigm Corporation

Inventor： David Cohen ,  Andrew May ,  Martin Pieprzyk ,  Brian Fowler ,  Kim Huat Lee ,  Jun Yan ,  Ming Fang Zhou ,  Seng Beng Ng

IPC: B01L99/00 ,  B01L3/00 ,  B01L9/00 ,  B01L7/00 ,  G01N1/40

CPC classification number: B01L3/5027 ,  B01L3/502738 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/12 ,  B01L2300/045 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0655 ,  G01N1/405

Abstract: The present invention includes microfluidic systems having a microfabricated cavity that may be covered with a removable cover, where the removable cover allows at least part of the opening of the microfabricated cavity to be exposed or directly accessed by an operator. The microfluidic systems comprise chambers, flow and control channels formed in elastomeric layers that may comprise PDMS. The removable cover comprises a thermoplastic base film bonded to an elastomer layer by an adhesive layer. When the removable cover is peeled off, the chamber is at least partially open to allow sample extraction from the chamber. The chamber may have macromolecular crystals formed inside or resulting contents from a PCR reaction. The invention also includes a method for making vias in elastomeric layers by using the removable cover. The invention further includes methods and devices for peeling the peelable cover or a removable component such as Integrated Heater Spreader.

公开(公告)号：US09383295B2

公开(公告)日：2016-07-05

申请号：US14477642

申请日：2014-09-04

Applicant: Fluidigm Corporation

Inventor： Martin Pieprzyk ,  Geoff Facer ,  Timothy Woudenberg ,  Brian Fowler

IPC: G01N21/64 ,  G01N1/28 ,  B01L3/00 ,  G01N21/05 ,  C12Q1/68 ,  B01L7/00 ,  G01N21/03

CPC classification number: G01N1/28 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/16 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  B33Y80/00 ,  C12Q1/686 ,  G01N21/05 ,  G01N21/6452 ,  G01N2021/0346 ,  Y10T137/7793 ,  Y10T436/2575

Abstract: Embodiments of the present invention provide improved microfluidic devices and related apparatus, systems, and methods. Methods are provided for reducing mixing times during use of microfluidic devices. Microfluidic devices and related methods of manufacturing are provided with increased manufacturing yield rates. Improved apparatus and related systems are provided for supplying controlled pressure to microfluidic devices. Methods and related microfluidic devices are provided for reducing dehydration of microfluidic devices during use. Microfluidic devices and related methods are provided with improved sample to reagent mixture ratio control. Microfluidic devices and systems are provided with improved resistance to compression fixture pressure induced failures. Methods and systems for conducting temperature controlled reactions using microfluidic devices are provided that reduce condensation levels within the microfluidic device. Methods and systems are provided for improved fluorescent imaging of microfluidic devices.

公开(公告)号：US09249459B2

公开(公告)日：2016-02-02

申请号：US14102331

申请日：2013-12-10

Applicant: Fluidigm Corporation

Inventor： Amy Hamilton ,  Min Lin ,  Alain Mir ,  Martin Pieprzyk

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6881 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2531/113 ,  C12Q2531/119 ,  C12Q2531/137 ,  C12Q2565/101 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/178

Abstract: The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.

Abstract translation: 本发明提供从小样品甚至单细胞分析基因组DNA和/或RNA的方法。 分析基因组DNA的方法可能需要全基因组扩增（WGA），然后对选定的靶核酸进行预扩增和扩增。 分析RNA的方法可能需要所需RNA的逆转录，然后对选定的靶核酸进行预扩增和扩增。

公开(公告)号：US20140193812A1

公开(公告)日：2014-07-10

申请号：US14102331

申请日：2013-12-10

Applicant: Fluidigm Corporation

Inventor： Amy Hamilton ,  Min Lin ,  Alain Mir ,  Martin Pieprzyk

IPC: C12Q1/68

CPC classification number: C12Q1/6881 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2531/113 ,  C12Q2531/119 ,  C12Q2531/137 ,  C12Q2565/101 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/178

Abstract: The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.

Abstract translation: 本发明提供从小样品甚至单细胞分析基因组DNA和/或RNA的方法。 分析基因组DNA的方法可能需要全基因组扩增（WGA），然后对选定的靶核酸进行预扩增和扩增。 分析RNA的方法可能需要所需RNA的逆转录，然后对选定的靶核酸进行预扩增和扩增。