公开(公告)号：US09933363B2

公开(公告)日：2018-04-03

申请号：US14461779

申请日：2014-08-18

Applicant: GE HEALTCARE BIO-SCIENCES CORP.

Inventor： Gaudenz Danuser ,  Paul C. Goodwin

IPC: G01N21/64 ,  G01N21/63 ,  G02B21/36 ,  G06T3/40

CPC classification number: G01N21/6428 ,  G01N21/636 ,  G01N21/6458 ,  G01N2021/6415 ,  G01N2021/6419 ,  G01N2201/126 ,  G02B21/365 ,  G02B21/367 ,  G06T3/4053

Abstract: Embodiments of the present invention are directed to imaging technologies, and, in particular, to an imaging system that detects relatively weak signals, over time, and that uses the detected signals to determine the positions of signal emitters. Particular embodiments of the present invention are directed to methods and systems for imaging fluorophore-labeled samples in order to produce images of the sample at resolutions significantly greater than the diffraction-limited resolution associated with optical microscopy. Embodiments of the present invention employ overlapping-emitter-image disambiguation to allow data to be collected from densely arranged emitters, which significantly decreases the data-collection time for producing intermediate images as well as the number of intermediate images needed to computationally construct high-resolution final images. Additional embodiments of the present invention employ hierarchical image-processing techniques to further resolve and interpret disambiguated images.