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1.发明申请公开(公告)号：US20150241351A1
公开(公告)日：2015-08-27
申请号：US14430147
申请日：2013-09-23
Applicant: GE Healthcare Bio-Sciences Corp.
Inventor： Jeremy Cooper , William M. Dougherty
CPC classification number: G01N21/6458 , G01B11/002 , G01N21/6486 , G01N2201/0633 , G01N2201/0636 , G02B21/06 , G02B21/14 , G02B21/16 , G02B21/361 , G02B21/365 , G02B21/367 , G02B27/58
Abstract: Methods and systems to resolve positions of sample components in fluorescence stochastic microscopy using three-dimensional structured illumination microscopy (“3D-SIM”) are disclosed. In one aspect, components of a sample specimen are labeled with fluorophores and weakly illuminated with a frequency of light to stochastically convert a subset of the fluorophores into an active state. The sample is then illuminated with a three-dimensional structured illumination pattern (“3D-SIP”) of excitation light that causes the activated fluorophores to fluoresce. As the 3D-SIP is incrementally moved within the volume of the sample and images are recorded, computational methods are used to process the images to locate and refine the locations of the activated fluorophores thereby generating a super-resolution image of sample components.
Abstract translation: 公开了使用三维结构照明显微镜（“3D-SIM”）在荧光随机显微镜中分析样品组分位置的方法和系统。 在一个方面，样品样品的成分用荧光团标记，并以光的频率被弱照射以将一部分荧光团随机转化为活性状态。 然后用引起活化的荧光团发荧光的激发光的三维结构照明模式（“3D-SIP”）照亮样品。 随着3D-SIP在样本的体积内逐渐移动并且记录图像，使用计算方法来处理图像以定位和细化活化的荧光团的位置，从而生成样品组分的超分辨率图像。
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2.发明授权公开(公告)号：US09581548B2
公开(公告)日：2017-02-28
申请号：US14430147
申请日：2013-09-23
Applicant: GE Healthcare Bio-Sciences Corp.
Inventor： Jeremy Cooper , William M. Dougherty
CPC classification number: G01N21/6458 , G01B11/002 , G01N21/6486 , G01N2201/0633 , G01N2201/0636 , G02B21/06 , G02B21/14 , G02B21/16 , G02B21/361 , G02B21/365 , G02B21/367 , G02B27/58
Abstract: Methods and systems to resolve positions of sample components in fluorescence stochastic microscopy using three-dimensional structured illumination microscopy (“3D-SIM”) are disclosed. In one aspect, components of a sample specimen are labeled with fluorophores and weakly illuminated with a frequency of light to stochastically convert a subset of the fluorophores into an active state. The sample is then illuminated with a three-dimensional structured illumination pattern (“3D-SIP”) of excitation light that causes the activated fluorophores to fluoresce. As the 3D-SIP is incrementally moved within the volume of the sample and images are recorded, computational methods are used to process the images to locate and refine the locations of the activated fluorophores thereby generating a super-resolution image of sample components.
Abstract translation: 公开了使用三维结构照明显微镜（“3D-SIM”）在荧光随机显微镜中分析样品组分位置的方法和系统。 在一个方面，样品样品的成分用荧光团标记，并以光的频率被弱照射以将一部分荧光团随机转化为活性状态。 然后用引起活化的荧光团发荧光的激发光的三维结构照明模式（“3D-SIP”）照亮样品。 随着3D-SIP在样本的体积内逐渐移动并且记录图像，使用计算方法来处理图像以定位和细化活化的荧光团的位置，从而生成样品组分的超分辨率图像。
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