公开(公告)号：US11312741B2

公开(公告)日：2022-04-26

申请号：US16094508

申请日：2017-04-19

Applicant: GLYCOM A/S

Inventor： Pierre Chassagne ,  Nikolay Khanzhin ,  Martin Matwiejuk ,  Markus Jondelius Hederos ,  Niels Banke

IPC: C07H13/04 ,  B01D61/02 ,  B01D61/14 ,  B01D61/58 ,  C07H1/06 ,  C12P19/28 ,  B01D15/36 ,  B01J41/07 ,  B01D61/16 ,  B01J39/05 ,  B01D15/18 ,  B01J20/20 ,  G01N30/96 ,  C12P19/26 ,  G01N30/02

Abstract: The invention relates to a method for obtaining an N-acetylglucosamine containing neutral oligosaccharide from a fermentation broth, wherein said oligosaccharide is produced by culturing a genetically modified microorganism capable of producing said oligosaccharide from an internalized carbohydrate precursor, comprising the steps of: i) ultrafiltration (UF), preferably to separate biomass from the broth, ii) nanofiltration (NF), preferably to concentrate said oligosaccharide in the broth and/or reduce an inorganic salt content of the broth, and iii) treating the broth with an ion exchange resin, preferably to remove charged materials, and/or subjecting the broth to chromatography, preferably to remove hydrophobic impurities.