公开(公告)号：US07429484B2

公开(公告)日：2008-09-30

申请号：US11331855

申请日：2006-01-13

Applicant: Hermann Oppermann ,  Thangavel Kuberasampath ,  David C. Rueger ,  Engin Ozkaynak

Inventor： Hermann Oppermann ,  Thangavel Kuberasampath ,  David C. Rueger ,  Engin Ozkaynak

IPC: C12N5/10 ,  C12N1/11 ,  C12N1/13 ,  C12N1/21 ,  C12N1/19 ,  C12N15/11 ,  C12N15/12

CPC classification number: C07K14/51 ,  A61K38/00

Abstract: Disclosed are 1) osteogenic devices comprising a matrix containing osteogenic protein and methods of inducing endochondral bone growth in mammals using the devices; 2) amino acid sequence data, amino acid composition, solubility properties, structural features, homologies and various other data characterizing osteogenic proteins, 3) methods of producing osteogenic proteins using recombinant DNA technology, and 4) osteogenically and chondrogenically active synthetic protein constructs.

Abstract translation: 公开的是1）包含含有成骨蛋白的基质的成骨装置和使用该装置在哺乳动物中诱导软骨内骨生长的方法; 2）氨基酸序列数据，氨基酸组成，溶解性，结构特征，同源性和各种其他数据表征成骨蛋白，3）使用重组DNA技术生产成骨蛋白的方法，以及4）成骨和软骨形成活性合成蛋白质构建体。

公开(公告)号：US20080227956A1

公开(公告)日：2008-09-18

申请号：US11824672

申请日：2007-07-02

Applicant: William K. Jones ,  Ronald F. Tucker ,  David C. Rueger ,  Hermann Oppermann ,  Engin Ozkaynak ,  Thangavel Kuberasampath

Inventor： William K. Jones ,  Ronald F. Tucker ,  David C. Rueger ,  Hermann Oppermann ,  Engin Ozkaynak ,  Thangavel Kuberasampath

IPC: C07K14/435

CPC classification number: C07K16/22 ,  A23C9/20 ,  A23L33/19 ,  A61F2310/00365 ,  A61K6/0017 ,  A61K38/17 ,  A61K38/1703 ,  A61K38/1875 ,  A61K48/00 ,  A61L27/227 ,  A61L27/24 ,  C07K14/495 ,  C07K14/51 ,  G01N33/6893 ,  G01N2500/10 ,  Y10S435/975 ,  Y10S436/811 ,  Y10S436/815 ,  A61K2300/00 ,  C08L89/00

Abstract: Disclosed are novel compositions of morphogenic proteins constituting soluble forms of these proteins, antibodies that distinguish between soluble and mature forms, and method for producing these morphogenic proteins and antibodies.

Abstract translation: 公开了构成这些蛋白质的可溶形式的形态发生蛋白质的新型组合物，区分可溶性和成熟形式的抗体，以及用于产生这些形态发生蛋白质和抗体的方法。

公开(公告)号：US07268208B2

公开(公告)日：2007-09-11

申请号：US10122026

申请日：2002-04-12

Applicant: William K. Jones ,  Ronald F. Tucker ,  David C. Rueger ,  Hermann Oppermann ,  Engin Ozkaynak ,  Thangavel Kubersmith

Inventor： William K. Jones ,  Ronald F. Tucker ,  David C. Rueger ,  Hermann Oppermann ,  Engin Ozkaynak ,  Thangavel Kubersmith

IPC: C07K4/12 ,  C07K14/51

CPC classification number: C07K16/22 ,  A23C9/20 ,  A23L33/19 ,  A61F2310/00365 ,  A61K6/0017 ,  A61K38/17 ,  A61K38/1703 ,  A61K38/1875 ,  A61K48/00 ,  A61L27/227 ,  A61L27/24 ,  C07K14/495 ,  C07K14/51 ,  G01N33/6893 ,  G01N2500/10 ,  Y10S435/975 ,  Y10S436/811 ,  Y10S436/815 ,  A61K2300/00 ,  C08L89/00

Abstract: Disclosed are novel compositions of morphogenic proteins constituting soluble forms of these proteins, antibodies that distinguish between soluble and mature forms, and method for producing these morphogenic proteins and antibodies.

Abstract translation: 公开了构成这些蛋白质的可溶形式的形态发生蛋白质的新型组合物，区分可溶性和成熟形式的抗体，以及用于产生这些形态发生蛋白质和抗体的方法。

公开(公告)号：US20070166353A1

公开(公告)日：2007-07-19

申请号：US11643552

申请日：2006-12-20

Applicant: Hermann Oppermann ,  Engin Ozkaynak ,  Thangavel Kuberasampath ,  David Rueger ,  Roy Pang

Inventor： Hermann Oppermann ,  Engin Ozkaynak ,  Thangavel Kuberasampath ,  David Rueger ,  Roy Pang

IPC: A61F2/02

CPC classification number: C07K14/47 ,  C07K5/0806 ,  C07K5/101

Abstract: Disclosed are (1) osteogenic devices comprising a matrix containing substantially pure natural-sourced mammalian osteogenic protein; (2) DNA and amino acid sequences for novel polypeptide chains useful as subunits of dimeric osteogenic proteins; (3) vectors carrying sequences encoding these novel polypeptide chains and host cells transfected with these vectors; (4) methods of producing these polypeptide chains using recombinant DNA technology; (5) antibodies specific for these novel polypeptide chains; (6) osteogenic devices comprising these recombinantly produced proteins in association with an appropriate carrier matrix; and (7) methods of using the osteogenic devices to mimic the natural course of endochondral bone formation in mammals.

Abstract translation: 公开的是（1）包含含有基本上纯天然来源的哺乳动物成骨蛋白质的基质的成骨装置; （2）可用作二聚成骨蛋白亚基的新型多肽链的DNA和氨基酸序列; （3）携带编码这些新型多肽链的序列的载体和用这些载体转染的宿主细胞; （4）使用重组DNA技术生产这些多肽链的方法; （5）对这些新型多肽链具有特异性的抗体; （6）包含这些重组产生的蛋白质与合适的载体基质相结合的成骨装置; 和（7）使用成骨装置来模拟哺乳动物软骨内骨形成的自然过程的方法。

公开(公告)号：US07105638B1

公开(公告)日：2006-09-12

申请号：US08014096

申请日：1993-02-04

Applicant: James S. Huston ,  Marc F. Charette ,  Charles M. Cohen ,  Roberto Crea ,  Peter C. Keck ,  Hermann Oppermann ,  David C. Rueger ,  Richard J. Ridge

Inventor： James S. Huston ,  Marc F. Charette ,  Charles M. Cohen ,  Roberto Crea ,  Peter C. Keck ,  Hermann Oppermann ,  David C. Rueger ,  Richard J. Ridge

IPC: C07K16/46

CPC classification number: C07K14/485 ,  C07K14/575 ,  C07K14/585 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/036 ,  C07K2319/50 ,  C07K2319/75 ,  C12N15/62

Abstract: Disclosed is a method for the isolation and purification of polypeptides expressed in host cells by recombinant DNA techniques. A fused polypeptide is produced containing a desired polypeptide fused to additional amino acids. The additional amino acids define a leader sequence having properties exploitable in purification, a hinge region, and a cleavage site. The hinge region is cysteine-free and has a secondary structure which serves to expose the cleavage site to a selected endopeptidase. The method of the invention involves the production of a fused polypeptide which may be efficiently isolated by exploiting the properties of the leader sequence, and then efficiently cleaved at the cleavage site in an appropriate aqueous environment by virtue of the influence of the hinge on the cleavage agent/cleavage site reaction and other properties of the fused polypeptide.

Abstract translation: 公开了通过重组DNA技术分离和纯化在宿主细胞中表达的多肽的方法。 产生融合的多肽，其含有与另外的氨基酸融合的所需多肽。 另外的氨基酸定义具有在纯化中可利用的性质，铰链区和切割位点的前导序列。 铰链区域是无半胱氨酸的并且具有用于将切割位点暴露于所选择的内肽酶的二级结构。 本发明的方法涉及产生融合多肽，其可以通过利用前导序列的性质而有效分离，然后由于铰链对切割的影响，在合适的水性环境中的切割位点处有效地切割 试剂/裂解位点反应和融合多肽的其他性质。

公开(公告)号：US20050096339A1

公开(公告)日：2005-05-05

申请号：US10865514

申请日：2004-06-09

Applicant: Thangavel Kuberasampath ,  Roy Pang ,  Hermann Oppermann ,  David Rueger ,  Charles Cohen ,  Engin Ozkaynak ,  John Smart

Inventor： Thangavel Kuberasampath ,  Roy Pang ,  Hermann Oppermann ,  David Rueger ,  Charles Cohen ,  Engin Ozkaynak ,  John Smart

IPC: A01N1/02 ,  A61F2/00 ,  A61K6/00 ,  A61K38/17 ,  A61K38/18 ,  A61L27/22 ,  A61L27/24 ,  C07K14/495 ,  C07K14/51 ,  C07K16/22 ,  A61K31/485

CPC classification number: A61K38/17 ,  A01N1/0226 ,  A61F2310/00365 ,  A61K38/1703 ,  A61K38/1875 ,  A61L27/227 ,  A61L27/24 ,  C07K14/495 ,  C07K14/51 ,  C07K16/22 ,  G01N2500/10 ,  A61K2300/00

Abstract: The present invention is directed to methods and compositions for alleviating tissue destructive effects associated with the inflammatory response to tissue injury in a mammal. The methods and compositions include administering a therapeutically effective concentration of a morphogen or morphogen-stimulating agent sufficient to alleviate immune cell-mediated tissue destruction.

Abstract translation: 本发明涉及减轻与哺乳动物对组织损伤的炎症反应相关的组织破坏作用的方法和组合物。 所述方法和组合物包括施用足以减轻免疫细胞介导的组织破坏的治疗有效浓度的形态发生剂或形态发生剂。

公开(公告)号：US06395883B1

公开(公告)日：2002-05-28

申请号：US08402542

申请日：1995-03-13

Applicant: William K. Jones ,  Ronald F. Tucker ,  David C. Rueger ,  Hermann Oppermann ,  Engin Ozkaynak ,  Thangavel Kuberasampath

Inventor： William K. Jones ,  Ronald F. Tucker ,  David C. Rueger ,  Hermann Oppermann ,  Engin Ozkaynak ,  Thangavel Kuberasampath

IPC: C07K14475

CPC classification number: C07K16/22 ,  A23C9/20 ,  A23L33/19 ,  A61F2310/00365 ,  A61K6/0017 ,  A61K38/17 ,  A61K38/1703 ,  A61K38/1875 ,  A61K48/00 ,  A61L27/227 ,  A61L27/24 ,  C07K14/495 ,  C07K14/51 ,  G01N33/6893 ,  G01N2500/10 ,  Y10S435/975 ,  Y10S436/811 ,  Y10S436/815 ,  A61K2300/00 ,  C08L89/00

Abstract: Disclosed are novel compositions of morphogenic proteins constituting soluble forms of these proteins, antibodies that distinguish between soluble and mature forms, and method for producing these morphogenic proteins and antibodies.

Abstract translation: 公开了构成这些蛋白质的可溶形式的形态发生蛋白质的新型组合物，区分可溶性和成熟形式的抗体，以及用于产生这些形态发生蛋白质和抗体的方法。

公开(公告)号：US06211146B1

公开(公告)日：2001-04-03

申请号：US08271556

申请日：1994-07-07

Applicant: Thangavel Kuberasampath ,  Roy H. L. Pang ,  Hermann Oppermann ,  David C. Rueger ,  Charles M. Cohen

Inventor： Thangavel Kuberasampath ,  Roy H. L. Pang ,  Hermann Oppermann ,  David C. Rueger ,  Charles M. Cohen

IPC: A61K3816

CPC classification number: G01N33/6872 ,  A01N1/0226 ,  A61F2310/00365 ,  A61K6/0032 ,  A61K38/17 ,  A61K38/1703 ,  A61K38/1875 ,  A61L27/227 ,  A61L27/24 ,  C07K14/495 ,  C07K14/51 ,  C07K16/22 ,  G01N2500/10 ,  A61K2300/00

Abstract: Disclosed are methods of utilizing a morphogenically active fragment of 60A protein to induce tissue morphogenesis, including methods for increasing a progenitor cell population in a mammal, methods for stimulating progenitor cells to differentiate and maintain their differentiated phenotype in vivo or in vitro, methods for inducing tissue-specific growth in vivo and methods for the replacement of diseased or damaged tissue in vivo.

Abstract translation: 公开了利用60A蛋白的形态发生活性片段诱导组织形态发生的方法，包括增加哺乳动物中祖细胞群的方法，用于刺激祖细胞在体内或体外分化和维持其分化表型的方法，诱导方法 体内的组织特异性生长和体内替换患病或受损组织的方法。

公开(公告)号：US5877305A

公开(公告)日：1999-03-02

申请号：US356786

申请日：1994-12-12

Applicant: James S. Huston ,  L. L. Houston ,  David B. Ring ,  Hermann Oppermann

Inventor： James S. Huston ,  L. L. Houston ,  David B. Ring ,  Hermann Oppermann

IPC: C12N15/09 ,  A61K39/395 ,  A61K49/00 ,  A61K51/00 ,  A61K51/08 ,  A61P35/00 ,  C07H21/04 ,  C07K16/22 ,  C07K16/28 ,  C07K16/30 ,  C07K16/32 ,  C07K16/46 ,  C07K19/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/13 ,  C12N15/62 ,  C12N15/66 ,  C12P21/02 ,  C12P21/08 ,  C12R1/19

CPC classification number: C07K16/32 ,  C07K16/22 ,  C07K16/30 ,  C07K16/3015 ,  C07K16/3069 ,  A61K2039/505 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/622 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/33 ,  C07K2319/55

Abstract: Disclosed is DNA encoding a single-chain Fv (sFv) polypeptide defining a binding site which exhibits the immunological binding properties of an immunoglobulin molecule which binds c-erbB-2 or a c-erbB-2-related tumor antigen, the sFv includes at least two polypeptide domains connected by a polypeptide linker spanning the distance between the C-terminus of one domain and the N-terminus of the other, the amino acid sequence of each of the polypeptide domains includes a set of complementarity determining regions (CDRs) interposed between a set of framework regions (FRs), the CDRs conferring immunological binding to the c-erbB-2 or c-erbB-2-related tumor antigen.

Abstract translation: 公开了编码单链Fv（sFv）多肽的DNA，其限定结合位点，其显示结合c-erbB-2或c-erbB-2相关肿瘤抗原的免疫球蛋白分子的免疫结合特性，sFv包括在 通过跨越一个结构域的C末端与另一个结构域的N末端之间的距离的多肽连接体连接的至少两个多肽结构域，每个多肽结构域的氨基酸序列包括一组互补决定区（CDR），其插入 在一组框架区域（FR）之间，CDRs赋予与c-erbB-2或c-erbB-2相关的肿瘤抗原的免疫学结合。

公开(公告)号：US5759815A

公开(公告)日：1998-06-02

申请号：US686550

申请日：1991-05-06

Applicant: Marc F. Charette ,  Zita A. Babickas ,  Hermann Oppermann

Inventor： Marc F. Charette ,  Zita A. Babickas ,  Hermann Oppermann

IPC: C07K14/49 ,  C12N15/18

CPC classification number: C07K14/49 ,  C07K2319/00

Abstract: Native, analog, and mutein species of A and B chains of platelet derived growth factor are produced in prokaryotes, purified, combined, and modified in vitro to produce various active AA, AB, and BB PDGF dimers.

Abstract translation: 在原核生物中产生血小板衍生生长因子的A和B链的天然，类似物和突变蛋白，在体外纯化，组合和修饰以产生各种活性AA，AB和BB PDGF二聚体。