公开(公告)号：US08124406B2

公开(公告)日：2012-02-28

申请号：US12979823

申请日：2010-12-28

Applicant: Kazuma Tomizuka ,  Hitoshi Yoshida ,  Kazunori Hanaoka ,  Mitsuo Oshimura ,  Isao Ishida ,  Yoshimi Kuroiwa

Inventor： Kazuma Tomizuka ,  Hitoshi Yoshida ,  Kazunori Hanaoka ,  Mitsuo Oshimura ,  Isao Ishida ,  Yoshimi Kuroiwa

IPC: C12N5/00 ,  C12N5/02 ,  C12N15/01 ,  C12N15/64 ,  C07H21/04

CPC classification number: A01K67/0275 ,  A01K67/0271 ,  A01K67/0276 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/01 ,  A01K2267/03 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/873 ,  C12N15/90 ,  C12N15/907 ,  C12N2800/30

Abstract: The present invention relates to a method for producing a modified foreign chromosome(s) or a fragment(s) thereof, which comprises the steps of: (a) preparing a microcell comprising a foreign chromosome(s) or a fragment(s) thereof, and transferring said foreign chromosome(s) or a fragment(s) into a cell with high homologous recombination efficiency through its fusion with said microcell; (b) in said cell with high homologous recombination efficiency, inserting a targeting vector by homologous recombination into a desired site of said foreign chromosome(s) or a fragment(s) thereof, and/or a desired site of a chromosome(s) derived from said cell with high homologous recombination efficiency, thereby marking said desired site; and (c) in said cell with high homologous recombination efficiency, causing deletion and/or translocation to occur at the marked site of said foreign chromosome(s) or a fragment(s) thereof.

Abstract translation: 本发明涉及一种生产修饰的外源染色体或其片段的方法，其包括以下步骤：（a）制备包含外来染色体或其片段的微细胞 并通过与所述微细胞融合将所述外源染色体或片段转移到具有高同源重组效率的细胞中; （b）在具有高同源重组效率的所述细胞中，通过同源重组将靶向载体插入所述外源染色体或其片段的所需位点和/或染色体的所需位点， 衍生自具有高同源重组效率的所述细胞，从而标记所述期望的位点; 和（c）在具有高同源重组效率的所述细胞中，导致在所述外源染色体或其片段的标记位点发生缺失和/或易位。

公开(公告)号：US08110671B2

公开(公告)日：2012-02-07

申请号：US11797904

申请日：2007-05-08

Applicant: Kazuma Tomizuka ,  Hitoshi Yoshida ,  Kazunori Hanaoka ,  Mitsuo Oshimura ,  Isao Ishida

Inventor： Kazuma Tomizuka ,  Hitoshi Yoshida ,  Kazunori Hanaoka ,  Mitsuo Oshimura ,  Isao Ishida

IPC: C07H21/04

CPC classification number: A01K67/0275 ,  A01K67/0271 ,  A01K67/0276 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/01 ,  A01K2267/03 ,  C07K16/00 ,  C07K16/18 ,  C07K2317/21 ,  C12N15/8509 ,  C12N15/90 ,  C12N2517/02 ,  C12N2800/30

Abstract: The specification relates to a method for producing a chimeric non-human animal, which comprises preparing a microcell containing a foreign chromosome(s) or a fragment(s) thereof and transferring the foreign chromosome(s) or fragment(s) thereof into a pluripotent cell by fusion with the microcell; a chimeric non-human animal which can be produced by the above method and its progeny; tissues and cells derived therefrom; and a method for using the same. Further, a pluripotent cell containing a foreign chromosome(s) or a fragment(s) thereof, a method for producing the same, and a method for using the same are also provided. Moreover, a pluripotent cell in which at least two endogenous genes are disrupted, and a method for producing the same by homologous recombination are provided.

Abstract translation: 本说明书涉及嵌合非人动物的制造方法，其包括制备含有外来染色体或其片段的微细胞，并将其外来的染色体或片段转移到 多细胞与微细胞融合; 可以通过上述方法及其后代产生的嵌合非人动物; 从其衍生的组织和细胞; 及其使用方法。 此外，还提供了含有外来染色体或其片段的多能细胞，其制备方法及其使用方法。 此外，提供其中至少两个内源基因被破坏的多能细胞，以及通过同源重组产生其的方法。

公开(公告)号：US07476536B2

公开(公告)日：2009-01-13

申请号：US10477471

申请日：2002-05-10

Applicant: Yoshimi Kuroiwa ,  Kazuma Tomizuka ,  Hitoshi Yoshida ,  Isao Ishida

Inventor： Yoshimi Kuroiwa ,  Kazuma Tomizuka ,  Hitoshi Yoshida ,  Isao Ishida

IPC: C12N15/63 ,  C12N21/02 ,  C12N15/00

CPC classification number: A01K67/0278 ,  A01K67/0275 ,  A01K67/0276 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/052 ,  A01K2217/054 ,  A01K2217/15 ,  A01K2227/105 ,  A01K2267/01 ,  A01K2267/0381 ,  A61K48/00 ,  A61K2039/505 ,  C07K16/00 ,  C07K16/243 ,  C07K2317/21 ,  C12N15/8509 ,  C12N15/87 ,  C12N15/90 ,  C12N2800/30

Abstract: The present invention relates to a human artificial chromosome which is genetically transmissible to the next generation with high efficiency and the method for using the same. More specifically, the present invention relates to: a human artificial chromosome in which an about 3.5 Mb to about 1 Mb region containing an antibody λ light chain gene derived from human chromosome 22 is bound to a chromosome fragment which is transmissible to a progeny through a germ line of a non-human animal, said chromosome fragment is derived from another human chromosome; a non-human animal carrying the human artificial chromosome and an offspring thereof; a method for producing the non-human animal; a method for producing a human antibody using the non-human animal or an offspring thereof; and a human antibody-producing mouse carrying the human artificial chromosome.

Abstract translation: 本发明涉及一种以高效率遗传传播到下一代的人类人造染色体及其使用方法。 更具体地，本发明涉及：人类人造染色体，其中含有源自人染色体22的抗体λ轻链基因的约3.5Mb至约1Mb区域与染色体片段结合，所述染色体片段可通过 生殖系的非人类动物，所述染色体片段衍生自另一个人染色体; 携带人类人造染色体的非人类动物及其后代; 一种生产非人动物的方法; 使用非人动物或其后代生产人抗体的方法; 和携带人类人造染色体的人抗体生产小鼠。

公开(公告)号：US20090007282A1

公开(公告)日：2009-01-01

申请号：US12116294

申请日：2008-05-07

Applicant: Kazuma TOMIZUKA ,  Hitoshi Yoshida ,  Kazunori Hanaoka ,  Mitsuo Oshimura ,  Isao Ishida ,  Yoshimi Kuroiwa

Inventor： Kazuma TOMIZUKA ,  Hitoshi Yoshida ,  Kazunori Hanaoka ,  Mitsuo Oshimura ,  Isao Ishida ,  Yoshimi Kuroiwa

IPC: A01K67/027

CPC classification number: A01K67/0275 ,  A01K67/0271 ,  A01K67/0276 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/01 ,  A01K2267/03 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/873 ,  C12N15/90 ,  C12N15/907 ,  C12N2800/30

Abstract: The present invention relates to a method for producing a modified foreign chromosome(s) or a fragment(s) thereof, which comprises the steps of: (a) preparing a microcell comprising a foreign chromosome(s) or a fragment(s) thereof, and transferring said foreign chromosome(s) or a fragment(s) into a cell with high homologous recombination efficiency through its fusion with said microcell; (b) in said cell with high homologous recombination efficiency, inserting a targeting vector by homologous recombination into a desired site of said foreign chromosome(s) or a fragment(s) thereof, and/or a desired site of a chromosome(s) derived from said cell with high homologous recombination efficiency, thereby marking said desired site; and (c) in said cell with high homologous recombination efficiency, causing deletion and/or translocation to occur at the marked site of said foreign chromosome(s) or a fragment(s) thereof.

Abstract translation: 本发明涉及一种生产修饰的外源染色体或其片段的方法，其包括以下步骤：（a）制备包含外来染色体或其片段的微细胞 并通过与所述微细胞融合将所述外源染色体或片段转移到具有高同源重组效率的细胞中; （b）在具有高同源重组效率的所述细胞中，通过同源重组将靶向载体插入所述外源染色体或其片段的所需位点和/或染色体的所需位点， 衍生自具有高同源重组效率的所述细胞，从而标记所述期望的位点; 和（c）在具有高同源重组效率的所述细胞中，导致在所述外源染色体或其片段的标记位点发生缺失和/或易位。

公开(公告)号：US20080317743A1

公开(公告)日：2008-12-25

申请号：US12140009

申请日：2008-06-16

Applicant: Yoshimi Kuroiwa ,  Kazuma Tomizuka ,  Hitoshi Yoshida ,  Isao Ishida

Inventor： Yoshimi Kuroiwa ,  Kazuma Tomizuka ,  Hitoshi Yoshida ,  Isao Ishida

IPC: A61K39/395 ,  A01K67/027 ,  C12P21/08

CPC classification number: A01K67/0278 ,  A01K67/0275 ,  A01K67/0276 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/052 ,  A01K2217/054 ,  A01K2217/15 ,  A01K2227/105 ,  A01K2267/01 ,  A01K2267/0381 ,  A61K48/00 ,  A61K2039/505 ,  C07K16/00 ,  C07K16/243 ,  C07K2317/21 ,  C12N15/8509 ,  C12N15/87 ,  C12N15/90 ,  C12N2800/30

Abstract: The present invention relates to a human artificial chromosome which is genetically transmissible to the next generation with high efficiency and the method for using the same. More specifically, the present invention relates to: a human artificial chromosome in which an about 3.5 Mb to about 1 Mb region containing an antibody λ light chain gene derived from human chromosome 22 is bound to a chromosome fragment which is transmissible to a progeny through a germ line of a non-human animal, said chromosome fragment is derived from another human chromosome; a non-human animal carrying the human artificial chromosome and an offspring thereof; a method for producing the non-human animal; a method for producing a human antibody using the nonhuman animal or an offspring thereof; and a human antibody-producing mouse carrying the human artificial chromosome.

Abstract translation: 本发明涉及一种以高效率遗传传播到下一代的人类人造染色体及其使用方法。 更具体地，本发明涉及：人类人造染色体，其中含有源自人染色体22的抗体λ轻链基因的约3.5Mb至约1Mb区域与染色体片段结合，所述染色体片段可通过 生殖系的非人类动物，所述染色体片段衍生自另一个人染色体; 携带人类人造染色体的非人类动物及其后代; 一种生产非人动物的方法; 使用非人动物或其后代生产人抗体的方法; 和携带人类人造染色体的人抗体生产小鼠。

公开(公告)号：USD577855S1

公开(公告)日：2008-09-30

申请号：US29260896

申请日：2006-06-05

Applicant: Hitoshi Yoshida

Designer： Hitoshi Yoshida

公开(公告)号：US20060126208A1

公开(公告)日：2006-06-15

申请号：US11294996

申请日：2005-12-05

Applicant: Nobuhito Kitamura ,  Hitoshi Yoshida

Inventor： Nobuhito Kitamura ,  Hitoshi Yoshida

IPC: G11B5/09 ,  G11B15/12 ,  G11B5/596

CPC classification number: G11B20/1217 ,  G11B5/3967 ,  G11B5/59655 ,  G11B2005/0002 ,  G11B2020/1277 ,  G11B2020/1287 ,  G11B2020/1292 ,  G11B2220/2516

Abstract: Embodiments of the invention provide a magnetic disk drive and its control method capable of effectively using the unrecordable area which is caused due to the composite magnetic head's gap between the read head and the write head. In one embodiment, a disk drive comprises a composite magnetic head structured in such a manner that during write the write head WH reaches each servo area earlier than the read head RH, and a read write control unit to control the write and read done by the composite magnetic head. On the disk, common servo data SP to be used both during read and during write, write servo data SW to be used during write and read servo data SR to be used during read are recorded in this order in each servo area. During write, the read write control unit starts the write head recording data at the timing when reading write servo data SW from the servo area is completed.

公开(公告)号：US06632976B1

公开(公告)日：2003-10-14

申请号：US09033936

申请日：1998-03-02

Applicant: Kazuma Tomizuka ,  Hitoshi Yoshida ,  Kazunori Hanaoka ,  Mitsuo Oshimura ,  Isao Ishida

Inventor： Kazuma Tomizuka ,  Hitoshi Yoshida ,  Kazunori Hanaoka ,  Mitsuo Oshimura ,  Isao Ishida

IPC: A01K67027

CPC classification number: A01K67/0275 ,  A01K67/0271 ,  A01K67/0276 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/01 ,  A01K2267/03 ,  C07K16/00 ,  C07K16/18 ,  C07K2317/21 ,  C12N15/8509 ,  C12N15/90 ,  C12N2517/02 ,  C12N2800/30

Abstract: A chimeric, non-human animal can be produced by a method that entails providing a microcell that contains one or more foreign chromosomes or fragment(s) thereof and then fusing the microcell with a pluripotent cell, thereby introducing the foreign chromosome(s) or fragment(s) into the latter. The pluripotent cell thus obtained can be used to generate a chimeric, non-human animal, the cells, tissues, and/or progeny of which can be the source of a product, such as an antibody, that is associated with one or more genes on the foreign chromosome(s) or fragment(s).

Abstract translation: 嵌合的非人动物可以通过一种方法产生，该方法需要提供含有一个或多个外来染色体或其片段的微细胞，然后将微细胞与多能细胞融合，由此引入外源染色体或 片段进入后者。 由此获得的多能细胞可以用于产生嵌合的非人动物，其细胞，组织和/或后代可以是与一个或多个基因相关的产物例如抗体的来源 在外来染色体或片段上。

公开(公告)号：US06597874B2

公开(公告)日：2003-07-22

申请号：US09934291

申请日：2001-08-21

Applicant: Yukihiko Sugita ,  Masami Takase ,  Takaaki Ue ,  Hitoshi Yoshida

Inventor： Yukihiko Sugita ,  Masami Takase ,  Takaaki Ue ,  Hitoshi Yoshida

IPC: G03B1702

CPC classification number: G03B17/02

Abstract: In a camera having a camera body unit constituted by combining a plurality of units, the camera configuration is realized so as to allow to easily remove only a lens frame unit from the camera body unit before the other units (for example, a strobe unit, a finder unit, a film feed unit and the like) when the camera is disassembled to repair or replace the lens frame unit by constituting so that the lens frame unit having a photographing optical system can be mounted independently.

公开(公告)号：US06309428B1

公开(公告)日：2001-10-30

申请号：US09555979

申请日：2000-06-06

Applicant: Yusuke Niiori ,  Hiroyuki Katsukawa ,  Hitoshi Yoshida

Inventor： Yusuke Niiori ,  Hiroyuki Katsukawa ,  Hitoshi Yoshida

IPC: H01G900

CPC classification number: H01G9/155 ,  Y02E60/13 ,  Y10T29/417

Abstract: A process for producing an electrochemical capacitor including an organic electrolytic solution and electrode bodies immersed in the organic electrolytic solution, each comprising a polarizing electrode (composed mainly of a carbon material containing a partially oxidized, graphite-like microcrystalline carbon), a separator and a collector, in which capacitor each polarizing electrode gives rise to volume expansion when charged and volume contraction when discharged, is provided. The process includes: a step of placing each electrode body and the organic electrolytic solution in a cell container to form a unit cell, a step of placing the unit cell or a cell integrate obtained by electrically connecting a plurality of such unit cells, in a first capacitor container to constitute a first electrochemical capacitor, a step of subjecting the first electrochemical capacitor to charging-discharging cycles until the maximum value of the stress caused by the volume expansion of each polarizing electrode during charging becomes almost constant, and a step of transferring, after the maximum value of the stress has become almost constant, the unit cell or the cell integrate into a second capacitor container to constitute a second electrochemical capacitor. The process can provide an electrochemical capacitor which causes no plastic deformation of the capacitor container and which is superior in durability and reliability.

Abstract translation: 一种生产电化学电容器的方法，该电化学电容器包括有机电解液和浸在有机电解液中的电极体，每个包括极化电极（主要由含有部分氧化的石墨状微晶碳的碳材料组成），隔膜和 集电器，其中每个极化电极的电容器在充电时产生体积膨胀，并且在放电时产生体积收缩。 该方法包括：将每个电极体和有机电解溶液放置在电池容器中以形成单位电池的步骤，将电池单元电池或电池组合放置在多个这样的单电池中的步骤， 构成第一电化学电容器的第一电容器容器，对第一电化学电容器进行充放电循环的步骤，直到由充电前的各极化电极的体积膨胀引起的应力的最大值几乎恒定为止， 在应力的最大值变得几乎恒定之后，单电池或电池集成到第二电容器容器中以构成第二电化学电容器。 该方法可以提供电容器容器，其不会导致电容器容器的塑性变形，并且其耐久性和可靠性优异。