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公开(公告)号：US09554095B2
公开(公告)日：2017-01-24
申请号：US13832509
申请日：2013-03-15
Applicant: INTELLIGENT BIO-SYSTEMS, INC.
Inventor： David Ray Stoops , Phillip Alan Veatch , Steven Jeffrey Gordon
Abstract: Imagers and alignment methods for use by imagers imaging deoxyribonucleic acid (DNA) fragments on a flow cell are disclosed. The imagers capture intensity values at DNA fragment bead locations in tiles with each tile having a reference location in the flow cell. Flow cells may be aligned by obtaining a dark field image of each tile during a first imaging session, identifying dark field constellations of bead locations within two separate tiles during the first imaging session, identifying corresponding constellations during a second imaging session, altering the reference location of at least one tile during the second imaging session to correct for a linear offset in the corresponding constellations, and applying at least one correction factor for reading out intensity values from the imager for the bead locations in the flow cell to correct for an angular offset determined from offsets in the corresponding constellations.
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公开(公告)号：US09591268B2
公开(公告)日：2017-03-07
申请号：US13832509
申请日：2013-03-15
Applicant: INTELLIGENT BIO-SYSTEMS, INC.
Inventor： David Ray Stoops , Phillip Alan Veatch , Steven Jeffrey Gordon
CPC classification number: G06T7/0012 , G01N15/14 , G01N2015/0038 , G01N2015/144 , G02B21/10 , G02B21/16 , G02B21/365 , G06T7/73 , G06T2200/32 , G06T2207/10056 , G06T2207/30024 , H04N7/18
Abstract: Imagers and alignment methods for use by imagers imaging deoxyribonucleic acid (DNA) fragments on a flow cell are disclosed. The imagers capture intensity values at DNA fragment bead locations in tiles with each tile having a reference location in the flow cell. Flow cells may be aligned by obtaining a dark field image of each tile during a first imaging session, identifying dark field constellations of bead locations within two separate tiles during the first imaging session, identifying corresponding constellations during a second imaging session, altering the reference location of at least one tile during the second imaging session to correct for a linear offset in the corresponding constellations, and applying at least one correction factor for reading out intensity values from the imager for the bead locations in the flow cell to correct for an angular offset determined from offsets in the corresponding constellations.
Abstract translation: 公开了成像仪使用的成像仪和对准方法，其在流动池上成像脱氧核糖核酸（DNA）片段。 成像器捕获瓷砖中DNA片段珠位置的强度值，每个瓦片在流动池中具有参考位置。 可以通过在第一成像会话期间获得每个瓦片的暗场图像来对准流动池，在第一成像会话期间识别两个单独的瓦片内的珠粒位置的暗场星座，在第二成像会话期间识别相应的星座，改变参考位置 的第二成像会话期间的至少一个瓦片以校正相应星座中的线性偏移，以及应用至少一个校正因子来从成像器读出强度值以用于流动池中的珠子位置以校正角度偏移 从相应星座中的偏移确定。
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公开(公告)号：US20170132787A1
公开(公告)日：2017-05-11
申请号：US15412743
申请日：2017-01-23
Applicant: Intelligent Bio-Systems, Inc.
Inventor： David Ray Stoops , Phillip Alan Veatch , Steven Jeffrey Gordon
CPC classification number: G06T7/0012 , G01N15/14 , G01N2015/0038 , G01N2015/144 , G02B21/10 , G02B21/16 , G02B21/365 , G06T7/73 , G06T2200/32 , G06T2207/10056 , G06T2207/30024 , H04N7/18
Abstract: A DNA flow cell processing method including positioning the flow cell on a stage at a predetermined location relative to a camera, illuminating the flow cell from a side with a first light source to reflect light off the DNA fragment bead locations, obtaining a first image of the flow cell and identifying a first reference pattern of bead locations in the first image, moving at least one of the flow cell and the stage relative to the camera, attempting to reposition the stage at the predetermined location, obtaining a second image of the flow cell, identifying the first reference pattern in the second image, and evaluating a first offset, relative to the camera, between the first reference pattern in the first image and the first reference pattern in the second image.
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