公开(公告)号：US09512001B2

公开(公告)日：2016-12-06

申请号：US12970688

申请日：2010-12-16

Applicant: Huachang Lu ,  Guangshun Yi ,  Depu Chen ,  Lianghong Guo ,  Jing Cheng

Inventor： Huachang Lu ,  Guangshun Yi ,  Depu Chen ,  Lianghong Guo ,  Jing Cheng

IPC: G01N33/543 ,  B82Y15/00 ,  G01N33/58

CPC classification number: B82Y15/00 ,  G01N33/54326 ,  G01N33/588 ,  Y10T428/2991

Abstract: This invention provides nanometer-sized fluorescent magnetic particles and processes of making them. The nanoparticle has a core particle comprising a magnetic material and a fluorescent material, and the particle size is less than about 1 micrometer. The nanoparticles can be coated with an inorganic or organic layer and can be surface-modified. The nanoparticles can be used in many biological assays.

Abstract translation: 本发明提供纳米级荧光磁性颗粒及其制备方法。 纳米颗粒具有包含磁性材料和荧光材料的核心颗粒，并且其粒径小于约1微米。 纳米颗粒可以用无机或有机层涂覆并且可以进行表面改性。 纳米颗粒可用于许多生物测定。

公开(公告)号：US08784633B2

公开(公告)日：2014-07-22

申请号：US12667507

申请日：2007-07-04

Applicant: Guangxin Xiang ,  Liangbin Pan ,  Wanli Xing ,  Lihua Huang ,  Zhongyao Yu ,  Jing Zhu ,  Yuxiang Zhou ,  Jing Cheng

Inventor： Guangxin Xiang ,  Liangbin Pan ,  Wanli Xing ,  Lihua Huang ,  Zhongyao Yu ,  Jing Zhu ,  Yuxiang Zhou ,  Jing Cheng

IPC: G01N27/447 ,  G01N27/453

CPC classification number: G01N33/4836 ,  B01J2219/00653 ,  B01J2219/00743 ,  B01L3/502707 ,  B01L2300/0645 ,  B01L2400/0421 ,  B01L2400/0424

Abstract: A microelectrode sensing device includes a substrate and an array of microelectrode sensors. Each sensor includes a first conductive layer that at least partially conducts electricity. The first conductive layer is formed above the substrate and patterned to include a recording electrode that measures electrical activities of target cells. Each sensor also includes a second conductive layer that at least partially conducts electricity. The second conductive layer is elevated above the first layer and patterned to include multiple positioning electrodes arranged to define a sensing region above the recording electrode. The positioning electrodes are designed to generate an electric field pattern in the sensing region to move and confine the target cells to a sub-region of the sensing region that at least partially overlaps the recording electrode.

Abstract translation: 微电极感测装置包括基板和微电极传感器阵列。 每个传感器包括至少部分地导电的第一导电层。 第一导电层形成在衬底上并被图案化以包括测量靶细胞的电活动的记录电极。 每个传感器还包括至少部分地传导电的第二导电层。 第二导电层在第一层之上被升高并且被图案化以包括布置成限定记录电极上方的感测区域的多个定位电极。 定位电极被设计成在感测区域中产生电场图案以移动并将目标单元限制在至少部分地与记录电极重叠的感测区域的子区域上。

公开(公告)号：US20120202872A1

公开(公告)日：2012-08-09

申请号：US13389428

申请日：2010-08-06

Applicant: Wenyan Qin ,  Peng Dong ,  Tuo Deng ,  Cailing Ma ,  Keith Richard Mitchelson ,  Hao Wen ,  Jing Cheng

Inventor： Wenyan Qin ,  Peng Dong ,  Tuo Deng ,  Cailing Ma ,  Keith Richard Mitchelson ,  Hao Wen ,  Jing Cheng

IPC: A61K31/713 ,  C07H21/02 ,  G01N33/53 ,  C40B40/06 ,  C40B30/04 ,  C12Q1/68 ,  C07H21/04 ,  A61P35/00

CPC classification number: C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/136 ,  C12Q2600/158 ,  C12Q2600/178

Abstract: The invention provides methods and compositions for the diagnosis and prognosis of cervical intraepithelial neoplasia and cervical cancer. The methods comprise the step of determining the expression levels or genetic status of specific miRNAs.

Abstract translation: 本发明提供了用于宫颈上皮内瘤变和子宫颈癌的诊断和预后的方法和组合物。 该方法包括确定特异性miRNA的表达水平或遗传状态的步骤。

公开(公告)号：US08003063B2

公开(公告)日：2011-08-23

申请号：US12516999

申请日：2007-03-09

Applicant: Xiaosheng Guan ,  Min Guo ,  Rong Zhang ,  Dong Dong ,  Yuming Hu ,  Ting Cui ,  Jing Cheng

Inventor： Xiaosheng Guan ,  Min Guo ,  Rong Zhang ,  Dong Dong ,  Yuming Hu ,  Ting Cui ,  Jing Cheng

IPC: B01L3/00

CPC classification number: B01L3/502746 ,  B01L3/50273 ,  B01L7/52 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0487 ,  G01N35/08 ,  G01N35/10 ,  G01N2035/00356 ,  G01N2035/1032 ,  G01N2035/1034 ,  Y10T137/0396 ,  Y10T436/2575

Abstract: The invention is directed to microfluidic devices comprising at least two processing channels, wherein each of the processing channels comprises an inlet, an outlet, and a high-flow-resistant and hydrophilic conduit; a distributing channel, wherein the distributing channel comprises an upstream end and a downstream end, and is in fluid communication with each inlet of the processing channels via the high-flow-resistant and hydrophilic conduit; and a flushing channel, wherein the flushing channel comprises an upstream end and a downstream end, and is in fluid communication with each outlet of the processing channels. The invention also provides methods of using the microfluidic devices.

Abstract translation: 本发明涉及包括至少两个处理通道的微流体装置，其中每个处理通道包括入口，出口和高流阻和亲水导管; 分配通道，其中所述分配通道包括上游端和下游端，并且经由所述高阻流和亲水导管与所述处理通道的每个入口流体连通; 和冲洗通道，其中所述冲洗通道包括上游端和下游端，并且与所述处理通道的每个出口流体连通。 本发明还提供使用微流体装置的方法。

公开(公告)号：US07632674B2

公开(公告)日：2009-12-15

申请号：US10499098

申请日：2002-11-29

Applicant: Wanli Xing ,  Zhe Yu ,  Guangxin Xiang ,  Liangbin Pan ,  Jing Cheng

Inventor： Wanli Xing ,  Zhe Yu ,  Guangxin Xiang ,  Liangbin Pan ,  Jing Cheng

IPC: C12M3/00

CPC classification number: G01N33/5438 ,  C12M35/02 ,  G01N2333/46 ,  G01N2500/10

Abstract: The present invention provides an apparatus for stimulating an animal cell and recording its physiological signal and methods of making and using thereof. The purpose of the present invention is to provide an apparatus for stimulating an animal cell and recording its physiological signal that is efficient, convenient, and accurate. The apparatus for stimulating an animal cell and recording its physiological signal of the present invention comprises a poor conductive substrate, wherein on at least one surface of the substrate is provided at least one unit conductive polymer layer and at least one good conductive microelectrode.

Abstract translation: 本发明提供一种用于刺激动物细胞并记录其生理信号的装置及其制造和使用方法。 本发明的目的是提供一种用于刺激动物细胞并记录其生理信号的装置，其有效，方便和准确。 用于刺激动物细胞并记录其本发明的生理信号的装置包括差的导电基底，其中在基底的至少一个表面上提供至少一个单位导电聚合物层和至少一个良好的导电微电极。

公开(公告)号：US20090143245A1

公开(公告)日：2009-06-04

申请号：US12093504

申请日：2006-02-20

Applicant: Huafang Gao ,  Ze Li ,  Dong Wang ,  Yanhua Liu ,  Xiang Liu ,  Yangzhou Jiang ,  Chuanzan Zhao ,  Li Li ,  Gengxin Lan ,  Tao Guo ,  Bin Cai ,  Wanli Xing ,  Yuxiang Zhou ,  Jing Cheng

Inventor： Huafang Gao ,  Ze Li ,  Dong Wang ,  Yanhua Liu ,  Xiang Liu ,  Yangzhou Jiang ,  Chuanzan Zhao ,  Li Li ,  Gengxin Lan ,  Tao Guo ,  Bin Cai ,  Wanli Xing ,  Yuxiang Zhou ,  Jing Cheng

IPC: C40B30/10 ,  C40B40/08 ,  C40B50/06

CPC classification number: C12Q1/6881 ,  C12Q1/6837 ,  C12Q2600/156

Abstract: The present invention provides a microarray for detecting a genotype at a polymorphic site in a plurality of nucleic acid samples, comprising a first set of nucleic acid fragments derived from the samples and a second set of nucleic acid fragments derived from a plurality of references immobilized thereon. The invention also provides a microarray comprising a set of nucleic acid fragments immobilized on the surface of the microarray, wherein the nucleic acid fragments are derived from the samples by amplifying a region in the sample containing the polymorphism through asymmetric PCR amplification. Methods of using and making the microarrays are also provided.

Abstract translation: 本发明提供了用于检测多个核酸样品中多态性位点处的基因型的微阵列，其包含衍生自样品的第一组核酸片段和衍生自多个固定在其上的参考文献的第二组核酸片段 。 本发明还提供了一种微阵列，其包含固定在微阵列表面上的一组核酸片段，其中通过不对称PCR扩增扩增含有多态性的样品中的区域，来从样品衍生出核酸片段。 还提供了使用和制造微阵列的方法。

公开(公告)号：US20080252954A1

公开(公告)日：2008-10-16

申请号：US11795660

申请日：2005-06-02

Applicant: Xianhua Wang ,  Hui Xu ,  Jianxin Ye ,  Yelei Sun ,  Guoliang Huang ,  Jing Cheng

Inventor： Xianhua Wang ,  Hui Xu ,  Jianxin Ye ,  Yelei Sun ,  Guoliang Huang ,  Jing Cheng

IPC: G02B26/08

CPC classification number: G01N21/6458 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6421 ,  G01N2201/10 ,  G01N2201/103

Abstract: The invention provides a laser microarray scanner for microarray scanning, comprising an optical system, a scanning platform, and a data processing system. During scanning, the optical system remains fixed, and the microarray placed on the scanning platform moves relative to the optical system. The microarray scanner disclosed herein has high scanning speed, high sensitivity, high resolution, and high signal-to-noise ratio, thus is ideal for use in microarray scanning

Abstract translation: 本发明提供一种用于微阵列扫描的激光微阵列扫描器，包括光学系统，扫描平台和数据处理系统。 在扫描期间，光学系统保持固定，并且放置在扫描平台上的微阵列相对于光学系统移动。 本文公开的微阵列扫描器具有高扫描速度，高灵敏度，高分辨率和高信噪比，因此是理想的用于微阵列扫描

公开(公告)号：US20080124251A1

公开(公告)日：2008-05-29

申请号：US11661019

申请日：2004-11-22

Applicant: Renyuan Chen ,  Guoqing Wang ,  Feijun Xian ,  Wanli Xing ,  Xianhua Wang ,  Jing Cheng

Inventor： Renyuan Chen ,  Guoqing Wang ,  Feijun Xian ,  Wanli Xing ,  Xianhua Wang ,  Jing Cheng

IPC: B01L9/06 ,  B01L11/00 ,  B01L7/02

CPC classification number: G01N1/40 ,  G01N2001/2866

Abstract: The present invention provides a sample preparation device for extracting drug residue. It comprises a case, in which a water bath, a vortex mixer, and a homogenizer are integrated. The case also has on it an integrated control panel for controlling heating temperature of the water bath and homogenization time of the homogenizer.

Abstract translation: 本发明提供了用于提取药物残留物的样品制备装置。 它包括其中集成了水浴，涡流混合器和均化器的壳体。 该情况还具有用于控制水浴的加热温度和均化器的均化时间的集成控制面板。

公开(公告)号：US20070042367A1

公开(公告)日：2007-02-22

申请号：US10547742

申请日：2003-05-06

Applicant: Shengce Tao ,  Jing Cheng ,  Xuemei Ma ,  Qiong Zhang ,  Yuxiang Zhou

Inventor： Shengce Tao ,  Jing Cheng ,  Xuemei Ma ,  Qiong Zhang ,  Yuxiang Zhou

IPC: C12Q1/68 ,  C12M1/34

CPC classification number: B01L3/508 ,  B01J2219/00385 ,  B01J2219/00527 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00722 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/163 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  C12Q2547/101 ,  C12Q2531/131

Abstract: This invention relates generally to the field of nucleic acid detection. In particular, the invention provides a lab-on-chip system for analyzing a nucleic acid, which system comprises, inter alia, controllably closed space, and a target nucleic acid can be prepared and/or amplified, and hybridized to a nucleic acid probe, and the hybridization signal can be acquired if desirable, in the controllably closed space without any material exchange between the controllably closed space and the outside environment. Methods for analyzing a nucleic acid using the lab-on-chip system is also provided.

Abstract translation: 本发明一般涉及核酸检测领域。 特别地，本发明提供了用于分析核酸的片上实验室系统，该系统尤其包括可控制的封闭空间，并且靶核酸可以制备和/或扩增，并与核酸探针杂交 ，并且如果需要，可以在可控制的封闭空间中获取杂交信号，而在可控制的封闭空间和外部环境之间没有任何物质交换。 还提供了使用片上实验室系统分析核酸的方法。

公开(公告)号：US20060124152A1

公开(公告)日：2006-06-15

申请号：US10524903

申请日：2002-12-10

Applicant: Gang Li ,  Wanli Xing ,  Ruige Wu ,  Min Guo ,  Jing Cheng

Inventor： Gang Li ,  Wanli Xing ,  Ruige Wu ,  Min Guo ,  Jing Cheng

IPC: B08B3/10

CPC classification number: B08B3/12 ,  C12Q1/6813 ,  C12Q2523/301

Abstract: A method and apparatus for washing solid substrate with ultrasonic wave after hybridization reaction are provided. The method includes (1) putting the solid substrate into a container having a washing solution, on which the hybridization reaction has been completed, (2) using an ultrasonic generator to produce a certain strength of ultrasonic wave and transmit it into the washing solution, (3) washing the substrate by means of the cavitation effect being produced in the washing solution by the ultrasonic wave. Since the cavitation bubbles in the washing solution can produce intensive efflux and local microslipstream against the solid surface, evidently reducing the liquid surface tension and friction and enhance the liquid flow, non-specific adsorbate and deposit bound weakly on the solid substrate can be washed down rapidly.

Abstract translation: 提供了一种用于在杂交反应之后用超声波洗涤固体底物的方法和装置。 该方法包括：（1）将固体基质置于具有杂交反应完成的洗涤液的容器中，（2）使用超声波发生器产生一定强度的超声波并将其输送到洗涤液中， （3）通过超声波在洗涤液中产生的空化作用洗涤基板。 由于洗涤液中的气穴气泡能够对固体表面产生强烈的流出和局部微滑流，明显降低了液面张力和摩擦力，增强了液体流动，固体基质上弱结合的非特异性吸附物和沉积物可以被冲洗掉 迅速