公开(公告)号：US20230175886A1

公开(公告)日：2023-06-08

申请号：US17920054

申请日：2021-04-23

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Holger Birk ,  Frank Hecht ,  Luis Alvarez ,  Bernd Widzgowski

IPC: G01J1/44 ,  G01N21/64 ,  G02B21/00

CPC classification number: G01J1/44 ,  G01N21/6408 ,  G01N21/6458 ,  G02B21/0076 ,  G02B21/0084 ,  G01J2001/442

Abstract: An apparatus is configured to count N-photon events within a time-dependent sequence of events of interactions of a plurality of photons with a light sensitive detector. The apparatus includes a signal-processing device and the light sensitive detector. An N-photon event represents an occurrence of at least N timely overlapping single photon events. The light sensitive detector is adapted to generate a time-dependent digital signal comprising digital patterns representing the time-dependent sequence of events from the detection of the plurality of photons with the light sensitive detector. Each digital pattern in the digital signal comprises a digital pattern width having a continuous sequence of digital values representing at least one event of interaction of at least one photon with the light sensitive detector. The signal-processing device is adapted to identify N-photon events from the digital patterns in the digital signal in dependence from the respective digital pattern width.

公开(公告)号：US12243194B2

公开(公告)日：2025-03-04

申请号：US17636035

申请日：2020-10-08

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Luis Alvarez ,  Frank Hecht

IPC: G06T5/70 ,  G01N21/64 ,  G06T5/10 ,  G06T5/20 ,  G06T5/50

Abstract: The present invention relates to a method for generating a result image. The method comprises the steps of: acquiring a STED image of a sample, the STED image comprising pixels; calculating Fourier coefficients of arrival times for the pixels of the image, resulting in real coefficients representing a first image and in imaginary coefficients representing a second image; deriving an intensity image from the STED image; applying a spatial filter to the first image, the second image and the intensity image, resulting in a filtered first image, a filtered second image, and filtered intensity image, respectively; calculating an image G based on the filtered first image and the filtered intensity image; calculating an image S based on the filtered second image and the filtered intensity image; and calculating a result image based on the image G and the image S.