公开(公告)号：US20220137075A1

公开(公告)日：2022-05-05

申请号：US17470596

申请日：2021-09-09

Applicant: Labrador Diagnostics LLC

Inventor： Mark Dayel ,  Samartha Anekal ,  Paul Patel ,  Ian Gibbons ,  Elizabeth A. Holmes

IPC: G01N33/86 ,  C12Q1/56 ,  G01N21/84 ,  G01N33/49 ,  G01N33/58

Abstract: Compositions and methods for measuring coagulation parameters using very small volumes of blood are provided. Advantageously, the methods described herein can be performed from a single drop of blood (about 20 μL) while generally leaving enough sample to perform other measurements, optionally in a multiplexed format. The methods and devices do not require a skilled operator and can be performed at the point of service, which can be an important feature for managing blood coagulation disorders and treatments thereof.

公开(公告)号：US11268130B2

公开(公告)日：2022-03-08

申请号：US16530322

申请日：2019-08-02

Applicant: Labrador Diagnostics LLC

Inventor： Douglas Matje ,  Ian Gibbons ,  Paul Patel ,  Elizabeth A. Holmes

IPC: C12Q1/60 ,  C12Q1/61 ,  G01N33/92 ,  C12Q1/26 ,  C12Q1/44

Abstract: Reagents, assays, methods, kits, devices, and systems for rapid measurement of cholesterol and cholesterol sub-fractions from a blood sample are provided. Total cholesterol, low density lipoprotein cholesterol, and high density lipoprotein cholesterol can be measured in a single assay using kinetic measurements, under conditions in which cholesterol sub-species are converted to a detectable product at distinct rates. The detectable product is measured at different times after assay initiation. A lipase, cholesterol esterase, cholesterol oxidase and a peroxidase may be used together to produce colored product in amounts directly proportional to the quantity of cholesterol converted. Methods for calculating very-low density lipoprotein cholesterol levels by further including triglyceride measurements are disclosed. Assays may be performed in a single reaction mixture, allowing more accurate and precise cholesterol determinations, including ratios of cholesterol sub-fractions to total cholesterol, at less expense, than would be expected by performing several different assays in different reaction mixtures.