公开(公告)号：US11649487B2

公开(公告)日：2023-05-16

申请号：US16986557

申请日：2020-08-06

Applicant: Labrador Diagnostics LLC

Inventor： Kamila Belhocine ,  Josephine Lee ,  Pranav Patel ,  Aaron Richardson ,  Scott Tabakman

IPC: C12Q1/686 ,  C12Q1/6844 ,  C12P19/34 ,  C07H1/00

CPC classification number: C12Q1/6844 ,  C07H1/00 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/686 ,  C12Q2525/151 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q1/686 ,  C12Q2525/151 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2527/101 ,  C12Q2527/101 ,  C12Q2531/119

Abstract: Methods and compositions for the amplification of nucleic acids and generation of concatemers are disclosed. Amplification methods provided herein may be performed under isothermal conditions. Methods and compositions may include reagents such nucleic acid polymerases and primers.

公开(公告)号：US11603558B2

公开(公告)日：2023-03-14

申请号：US16190625

申请日：2018-11-14

Applicant: Labrador Diagnostics LLC

Inventor： Kamila Belhocine ,  Josephine Lee ,  Pranav Patel ,  Aaron Richardson ,  Scott Tabakman

IPC: C12Q1/6844 ,  C12Q1/686 ,  C12P19/34 ,  C07H1/00

Abstract: Methods and compositions for the amplification of nucleic acids and generation of concatemers are disclosed. Amplification methods provided herein may be performed under isothermal conditions. Methods and compositions may include reagents such as nucleic acid polymerases and primers.

公开(公告)号：US20210024987A1

公开(公告)日：2021-01-28

申请号：US16986557

申请日：2020-08-06

Applicant: Labrador Diagnostics LLC

Inventor： Kamila Belhocine ,  Josephine Lee ,  Pranav Patel ,  Aaron Richardson ,  Scott Tabakman

IPC: C12Q1/6844 ,  C12P19/34 ,  C12Q1/686 ,  C07H1/00

Abstract: Methods and compositions for the amplification of nucleic acids and generation of concatemers are disclosed. Amplification methods provided herein may be performed under isothermal conditions. Methods and compositions may include reagents such nucleic acid polymerases and primers.

公开(公告)号：US10822648B1

公开(公告)日：2020-11-03

申请号：US15664769

申请日：2017-07-31

Applicant: Labrador Diagnostics LLC

Inventor： Pranav Patel ,  Indira Wu ,  Aaron Richardson ,  Zahra Kamila Belhocine ,  Josephine Lee ,  Scott Tabakman

IPC: C12Q1/68 ,  C12Q1/686 ,  C12Q1/6806

Abstract: Improved methods for amplifying target nucleic acid sequences are provided by 1) first amplifying the number of copies of target nucleic acid sequences in a sample by a first nucleic acid amplification method, and then 2) applying a second nucleic amplification method to the amplified sample, or aliquot thereof, further amplifying the number of copies of target sequences. In embodiments, a first nucleic acid amplification method is a thermocycling method, and a second nucleic acid amplification method is an isothermal method.

公开(公告)号：US11254960B2

公开(公告)日：2022-02-22

申请号：US14848220

申请日：2015-09-08

Applicant: Labrador Diagnostics LLC

Inventor： Kamila Belhocine ,  Pranav Patel ,  Josephine Lee ,  Aaron Richardson ,  Scott Tabakman

IPC: C12P19/34 ,  C12Q1/6827 ,  C12Q1/6844

Abstract: Methods and compositions for the amplification of nucleic acids are disclosed. Amplification methods provided herein may be performed under isothermal conditions. Methods and compositions may include reagents such as nucleic acid polymerases, ligases, and primers.

公开(公告)号：US20210147910A1

公开(公告)日：2021-05-20

申请号：US17083815

申请日：2020-10-29

Applicant: Labrador Diagnostics LLC

Inventor： Pranav Patel ,  Indira Wu ,  Aaron Richardson ,  Zahra Kamila Belhocine ,  Josephine Lee ,  Scott Tabakman

IPC: C12Q1/686 ,  C12Q1/6806

Abstract: Improved methods for amplifying target nucleic acid sequences are provided by 1) first amplifying the number of copies of target nucleic acid sequences in a sample by a first nucleic acid amplification method, and then 2) applying a second nucleic amplification method to the amplified sample, or aliquot thereof, further amplifying the number of copies of target sequences. In embodiments, a first nucleic acid amplification method is a thermocycling method, and a second nucleic acid amplification method is an isothermal method.