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公开(公告)号：US20230255157A1
公开(公告)日：2023-08-17
申请号：US17935438
申请日：2022-09-26
Applicant: Monsanto Technology LLC
Inventor： Christopher T. Bauer , David V. Butruille , Edward J. Cargill , Daniel Challis , Fenggao Dong , Fengxing Du , Brian W. Gardunia , Yang Ju Im , Jonathan C. Lamb , Piyaraj Nan Newton , Pierre Sehabiague , Ruth A. Wagner , Shengqiang Zhong
Abstract: The invention provides novel methods to genotype haploid embryos using molecular assays. For example, quantitative methods for genotyping are provided. The methods provided also include providing a plurality of haploid kernels, determining the genotype of the haploid embryo of said kernels by distinguishing its genotype from the endosperm genotype, selecting a kernel having a desired genotype and producing doubled haploid plant from the selected kernel.
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公开(公告)号：US11698345B2
公开(公告)日：2023-07-11
申请号：US16625386
申请日：2018-06-19
Applicant: Monsanto Technology LLC
Inventor： Wayne Brown , Michael Joseph Dayawon , David W. Finley , William Michael Fischer , Yang Ju Im , John Michael Jensen , Jeffrey Lawrence Kohne , Matthew J. Weis
CPC classification number: G01N21/85 , G01N1/04 , G01N1/286 , G01N35/04 , G01N2001/2873 , G01N2015/0019 , G01N2021/8592 , G01N2035/0496
Abstract: A seed sampling system is provided comprising an automated seed loading assembly operable to singulate seeds from a plurality of seeds or enable loading of individually stored seeds and an automated seed sampling assembly comprising at least one sampling module operable to remove tissue samples from one of the singulated seeds. The system also includes an automated seed transport assembly comprising at least one retention member operable to transfer the singulated seeds from at least one elevator unit of the seed loading assembly to the at least one sampling module of the seed sampling assembly. In connection therewith, the at least one sampling module includes multiple sampling locations, each associated with a sampler, where the at least one sampling module is operable to remove tissue samples from seeds at one of sampling locations while another one of the sampling locations is cleaned to remove residual seed tissue therefrom.
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公开(公告)号：US12298252B2
公开(公告)日：2025-05-13
申请号：US18201091
申请日：2023-05-23
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Wayne Brown , Michael Joseph Dayawon , David Finley , William Michael Fischer , Yang Ju Im , John Michael Jensen , Jeffrey Lawrence Kohne , Matthew J. Weis
Abstract: A seed sampling system is provided comprising an automated seed loading assembly operable to singulate seeds from a plurality of seeds or enable loading of individually stored seeds and an automated seed sampling assembly comprising at least one sampling module operable to remove tissue samples from one of the singulated seeds. The system also includes an automated seed transport assembly comprising at least one retention member operable to transfer the singulated seeds from at least one elevator unit of the seed loading assembly to the at least one sampling module of the seed sampling assembly. In connection therewith, the at least one sampling module includes multiple sampling locations, each associated with a sampler, where the at least one sampling module is operable to remove tissue samples from seeds at one of sampling locations while another one of the sampling locations is cleaned to remove residual seed tissue therefrom.
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公开(公告)号：US11483991B2
公开(公告)日：2022-11-01
申请号：US17049957
申请日：2019-04-25
Applicant: Monsanto Technology LLC
Inventor： Christopher T. Bauer , David V. Butruille , Edward J. Cargill , Daniel Challis , Fenggao Dong , Fengxing Du , Brian W. Gardunia , Yang Ju Im , Jonathan C. Lamb , Piyaraj Nan Newton , Pierre Sehabiague , Ruth A. Wagner , Shengqiang Zhong
Abstract: The invention provides novel methods to genotype haploid embryos using molecular assays. For example, quantitative methods for genotyping are provided. The methods provided also include providing a plurality of haploid kernels, determining the genotype of the haploid embryo of said kernels by distinguishing its genotype from the endosperm genotype, selecting a kernel having a desired genotype and producing doubled haploid plant from the selected kernel.
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