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公开(公告)号：US10663456B2
公开(公告)日：2020-05-26
申请号：US15746570
申请日：2016-07-06
Inventor： Hidenori Tani , Masaki Torimura , Hiroaki Sato
Abstract: The present invention provides means for evaluating harmfulness of a chemical substance before occurrence of cell death, that is, more quickly and sensitively compared to conventional methods wherein the remaining viable cell count is determined using a reductive coloring reagent after a period of time required for occurrence of cell death due to the chemical substance. A double-stranded RNA probe comprising an RNA strand labeled with a fluorescent dye A that emits fluorescence, and an RNA strand labeled with a fluorescent dye B that quenches emission from a fluorescent dye in the vicinity thereof, wherein the fluorescence is quenched in a double-stranded state due to occurrence of fluorescence resonance energy transfer (FRET) between the two kinds of fluorescent dyes. When the probe is introduced into a cell and the cell is in a normal state, the double-stranded RNA is quickly degraded by activity of intracellular ribonuclease to cause cancellation of the FRET state, allowing fluorescence emission of the fluorescent dye A. On the other hand, in cases where the cell is harmfully influenced, activity of intracellular ribonuclease is suppressed, so that the double-stranded RNA remains undegraded, and the fluorescent dye A remains quenched due to the influence of the fluorescent dye B. By detecting a decrease in the degradation rate of intracellular RNA based on this principle, harmfulness of a chemical substance can be quickly and sensitively evaluated before occurrence of cell death.
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Patent Agency Ranking
公开(公告)号：US09120071B2
公开(公告)日：2015-09-01
申请号：US13906818
申请日：2013-05-31
Inventor： Hiroshi Aoki , Masaki Torimura , Hiroaki Tao , Takashi Ikeda
CPC classification number: B01J19/0046 , B01J2219/00369 , B01J2219/00387 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00659 , B01L3/0244 , B01L3/0262 , B01L3/0268 , B01L9/54 , B01L9/547 , B01L2200/022 , B01L2300/0838 , G01N35/1067 , G01N35/1074 , Y10T436/2575
Abstract: A spotter that includes a plurality of spotting heads, each of the plurality of spotting heads having a discharging portion at a tip portion, the plurality of spotting heads form an m×n array (m, n>1) with m spotting heads arranged lengthwise and n spotting heads arranged crosswise; and a pitch varying mechanism configured to vary an array pitch of the plurality of spotting heads arrayed in a lengthwise direction and an array pitch of the plurality of spotting heads arrayed in a crosswise direction.
Abstract translation: 一种检测器，包括多个点样头，所述多个点样头中的每一个具有在尖端部分的排出部分，所述多个点样头形成m×n阵列（m，n> 1），其中m个点样头纵向排列 并且n个点头交叉排列; 以及变桨机构，被配置为改变沿横向方向排列的多个点样头的阵列间距和沿横向排列的多个点样头的阵列间距。
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公开(公告)号：US12117343B2
公开(公告)日：2024-10-15
申请号：US17904383
申请日：2020-12-10
Inventor： Yoshiyuki Teramoto , Masaki Torimura , Taizo Sano
CPC classification number: G01J3/443 , G01N21/255 , G01N21/31 , G01J2003/2859
Abstract: Provided is a sample analysis system including: a droplet device configured to intermittently introduce a sample to a measurement region set in plasma; a light emission detection device configured to detect light emission in the measurement region at a detection timing, the detection timing being set at a predetermined cycle in advance; and an analysis device configured to analyze the sample based on the detected light emission, wherein the analysis device is provided with: a distribution computing unit configured to compute a time-spatial light intensity distribution based on the detected light emission, the time-spatial light intensity distribution being a distribution of a light intensity according to the detection timing, a position in the measurement region, and a wavelength component of the light emission; and a characteristic specifying unit configured to compute, from the time-spatial light intensity distribution, a feature amount that correlates with a sample characteristic indicating a property of the sample and specify the sample characteristic based on the feature amount.
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公开(公告)号：US10533231B2
公开(公告)日：2020-01-14
申请号：US14438534
申请日：2013-09-18
Inventor： Sung Bae Kim , Masaki Torimura , Hiroaki Tao , Tetsuya Nakazato
IPC: C12N9/02 , C12Q1/6897 , C12Q1/66 , C07K14/72 , G01N33/535
Abstract: The invention relates to establishment of a series of artificial luciferases based on artificial amino acid sequences extracted by amino acid alignment of copepod-derived luciferase sequences in a database based on amino acid similarity. The invention provides high luminescence intensity, high luminescence stability, and a spectrum with increased wavelength as luminescence characteristics. A series of artificial luciferases (ALuc) was established. The group of ALucs has superior luminescence characteristics, such as an increase in luminescence intensity, an increase in luminescence stability, or an increase in wavelength of the luminescence spectrum, which were not obtained before. Further, by using the artificial luciferases (ALuc) of the invention, it is possible to provide a novel, superior bioassay system, such as a bioluminescent probe, two-hybrid assay, a luminescent capsule, or the like having improved measurement function.
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公开(公告)号：US10214766B2
公开(公告)日：2019-02-26
申请号：US15030281
申请日：2014-10-16
Inventor： Sung Bae Kim , Hiroshi Izumi , Hiroaki Tao , Masaki Torimura , Akihiro Wakisaka
IPC: C12Q1/66 , C07D241/38 , C12N9/02 , C07D487/04
Abstract: The invention relates to a bioluminescent substrate suitably usable in a series of artificial luciferases (ALuc), and the invention provides a wavelength-shifted spectrum with a selective high intensity luminescence and high luminescence stability obtained by the use of the substrate together with ALuc. The luminescent substrate for ALuc obtained by the invention can be included together with a suitable luminescence solution in a luminescence kit. The bioluminescent substrate for ALuc of the invention can exhibit unprecedented excellent luminescence specificity and functionality in the conventional bioluminescence probe, two-hybrid assay, bioluminescent capsule, and reporter gene assay.
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公开(公告)号：US20150284813A1
公开(公告)日：2015-10-08
申请号：US14438534
申请日：2013-09-18
Inventor： Sung Bae Kim , Masaki Torimura , Hiroaki Tao , Tetsuya Nakazato
IPC: C12Q1/68 , G01N33/535 , C12Q1/66 , C12N9/02 , C07K14/72
CPC classification number: C12Q1/6897 , C07K14/72 , C07K2319/21 , C07K2319/41 , C07K2319/42 , C07K2319/43 , C07K2319/61 , C07K2319/70 , C12N9/0069 , C12Q1/66 , C12Y113/12007 , G01N33/535 , G01N33/581
Abstract: The invention relates to establishment of a series of artificial luciferases based on artificial amino acid sequences extracted by amino acid alignment of copepod-derived luciferase sequences in a database based on amino acid similarity. The invention provides high luminescence intensity, high luminescence stability, and a spectrum with increased wavelength as luminescence characteristics. A series of artificial luciferases (ALuc) was established. The group of ALucs has superior luminescence characteristics, such as an increase in luminescence intensity, an increase in luminescence stability, or an increase in wavelength of the luminescence spectrum, which were not obtained before. Further, by using the artificial luciferases (ALuc) of the invention, it is possible to provide a novel, superior bioassay system, such as a bioluminescent probe, two-hybrid assay, a luminescent capsule, or the like having improved measurement function.
Abstract translation: 本发明涉及基于氨基酸相似性在数据库中建立基于人猿氨基酸序列的人工荧光素酶序列，所述人工氨基酸序列是通过桡足类衍生的萤光素酶序列的氨基酸比对提取的。 本发明提供高发光强度，高发光稳定性和具有增加的波长作为发光特性的光谱。 建立了一系列人造荧光素酶（ALuc）。 该组ALuc具有优异的发光特性，例如发光强度的增加，发光稳定性的增加或发光光谱的波长增加，这在以前未获得。 此外，通过使用本发明的人造荧光素酶（ALuc），可以提供具有改善的测量功能的新型优异的生物测定系统，例如生物发光探针，双杂交测定，发光胶囊等。
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