公开(公告)号：US10787650B2

公开(公告)日：2020-09-29

申请号：US15763779

申请日：2016-09-30

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Sung Bae Kim

IPC: C12N9/02 ,  C12N15/53 ,  C12N15/62 ,  C12N1/20 ,  C12N5/10 ,  C12Q1/66 ,  G01N33/543 ,  C12N15/09 ,  C12Q1/26 ,  C07K19/00 ,  G01N21/76 ,  G01N33/535

Abstract: The invention relates to provision of artificial bioluminescent enzymes. The invention provides for a polypeptide, including any one of amino acid sequences (i) to (iii) below, and having luciferase activity: (i) an amino acid sequence of SEQ ID NO: 1; (ii) an amino acid sequence of SEQ ID NO: 1 in which 1 to 10 amino acids are substituted, added, or deleted; or (iii) an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 1.

公开(公告)号：US10533231B2

公开(公告)日：2020-01-14

申请号：US14438534

申请日：2013-09-18

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Sung Bae Kim ,  Masaki Torimura ,  Hiroaki Tao ,  Tetsuya Nakazato

IPC: C12N9/02 ,  C12Q1/6897 ,  C12Q1/66 ,  C07K14/72 ,  G01N33/535

Abstract: The invention relates to establishment of a series of artificial luciferases based on artificial amino acid sequences extracted by amino acid alignment of copepod-derived luciferase sequences in a database based on amino acid similarity. The invention provides high luminescence intensity, high luminescence stability, and a spectrum with increased wavelength as luminescence characteristics. A series of artificial luciferases (ALuc) was established. The group of ALucs has superior luminescence characteristics, such as an increase in luminescence intensity, an increase in luminescence stability, or an increase in wavelength of the luminescence spectrum, which were not obtained before. Further, by using the artificial luciferases (ALuc) of the invention, it is possible to provide a novel, superior bioassay system, such as a bioluminescent probe, two-hybrid assay, a luminescent capsule, or the like having improved measurement function.

公开(公告)号：US10214766B2

公开(公告)日：2019-02-26

申请号：US15030281

申请日：2014-10-16

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Sung Bae Kim ,  Hiroshi Izumi ,  Hiroaki Tao ,  Masaki Torimura ,  Akihiro Wakisaka

IPC: C12Q1/66 ,  C07D241/38 ,  C12N9/02 ,  C07D487/04

Abstract: The invention relates to a bioluminescent substrate suitably usable in a series of artificial luciferases (ALuc), and the invention provides a wavelength-shifted spectrum with a selective high intensity luminescence and high luminescence stability obtained by the use of the substrate together with ALuc. The luminescent substrate for ALuc obtained by the invention can be included together with a suitable luminescence solution in a luminescence kit. The bioluminescent substrate for ALuc of the invention can exhibit unprecedented excellent luminescence specificity and functionality in the conventional bioluminescence probe, two-hybrid assay, bioluminescent capsule, and reporter gene assay.

公开(公告)号：US20150284813A1

公开(公告)日：2015-10-08

申请号：US14438534

申请日：2013-09-18

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Sung Bae Kim ,  Masaki Torimura ,  Hiroaki Tao ,  Tetsuya Nakazato

IPC: C12Q1/68 ,  G01N33/535 ,  C12Q1/66 ,  C12N9/02 ,  C07K14/72

CPC classification number: C12Q1/6897 ,  C07K14/72 ,  C07K2319/21 ,  C07K2319/41 ,  C07K2319/42 ,  C07K2319/43 ,  C07K2319/61 ,  C07K2319/70 ,  C12N9/0069 ,  C12Q1/66 ,  C12Y113/12007 ,  G01N33/535 ,  G01N33/581

Abstract: The invention relates to establishment of a series of artificial luciferases based on artificial amino acid sequences extracted by amino acid alignment of copepod-derived luciferase sequences in a database based on amino acid similarity. The invention provides high luminescence intensity, high luminescence stability, and a spectrum with increased wavelength as luminescence characteristics. A series of artificial luciferases (ALuc) was established. The group of ALucs has superior luminescence characteristics, such as an increase in luminescence intensity, an increase in luminescence stability, or an increase in wavelength of the luminescence spectrum, which were not obtained before. Further, by using the artificial luciferases (ALuc) of the invention, it is possible to provide a novel, superior bioassay system, such as a bioluminescent probe, two-hybrid assay, a luminescent capsule, or the like having improved measurement function.

Abstract translation: 本发明涉及基于氨基酸相似性在数据库中建立基于人猿氨基酸序列的人工荧光素酶序列，所述人工氨基酸序列是通过桡足类衍生的萤光素酶序列的氨基酸比对提取的。 本发明提供高发光强度，高发光稳定性和具有增加的波长作为发光特性的光谱。 建立了一系列人造荧光素酶（ALuc）。 该组ALuc具有优异的发光特性，例如发光强度的增加，发光稳定性的增加或发光光谱的波长增加，这在以前未获得。 此外，通过使用本发明的人造荧光素酶（ALuc），可以提供具有改善的测量功能的新型优异的生物测定系统，例如生物发光探针，双杂交测定，发光胶囊等。