公开(公告)号：US20220016630A1

公开(公告)日：2022-01-20

申请号：US17390404

申请日：2021-07-30

Applicant: Purdue Research Foundation

Inventor： Cagri A. Savran ,  Philip S. Low ,  Chun-Li Chang ,  Wanfeng Huang

IPC: B01L3/00 ,  G01N33/574 ,  G01N33/543 ,  G01N27/74

Abstract: A microfluidic detection system for micrometer-sized entities, such as biological cells, includes a detector component incorporating a plate with a plurality of opening, the plate separating two chambers, one in communication with a fluid source containing target entities bound to magnetic beads. The openings are sized to always permit passage of the magnetic beads therethrough into a lower one of the chambers and are further sized to always prevent passage of the target entities from the upper one of the chambers. The detector component further includes a magnet positioned to pull unbound magnetic beads through the openings and to capture target entities bound to magnetic beads on the surface of the plate. In a further feature, the microfluidic detection system is configured to pass target molecules through the plate to be bound to a functionalized surface of the lower chamber.

公开(公告)号：US20190143328A1

公开(公告)日：2019-05-16

申请号：US16095648

申请日：2017-04-24

Applicant: Purdue Research Foundation

Inventor： Cagri A. Savran ,  Chun-Li Chang ,  Wanfeng Huang ,  Onur GUR ,  Rohil JAIN

IPC: B01L3/00 ,  B03C1/01 ,  B03C1/033 ,  B03C1/28

Abstract: Microfluidic systems and methods are described for capturing magnetic target entities bound to one or more magnetic beads. The systems include a well array device that includes a substrate with a surface that has a plurality of wells arranged in one or more arrays on the surface. A first array of wells is arranged adjacent to a first location on the surface. A second and subsequent arrays, if present, are arranged sequentially on the surface at second and subsequent locations. When a liquid sample is added onto the substrate and caused to flow, the liquid sample will flow across the first array first and then flow across the second and subsequent arrays in sequential order. The wells in the first array each have a size that permits entry of only one target entity into the well and each well in the first array has approximately the same size.

公开(公告)号：US11173488B2

公开(公告)日：2021-11-16

申请号：US16095648

申请日：2017-04-24

Applicant: Purdue Research Foundation

Inventor： Cagri A. Savran ,  Chun-Li Chang ,  Wanfeng Huang ,  Onur Gur ,  Rohil Jain

IPC: B01L3/00 ,  B03C1/01 ,  B03C1/033 ,  B03C1/28

Abstract: Microfluidic systems and methods are described for capturing magnetic target entities bound to one or more magnetic beads. The systems include a well array device that includes a substrate with a surface that has a plurality of wells arranged in one or more arrays on the surface. A first array of wells is arranged adjacent to a first location on the surface. A second and subsequent arrays, if present, are arranged sequentially on the surface at second and subsequent locations. When a liquid sample is added onto the substrate and caused to flow, the liquid sample will flow across the first array first and then flow across the second and subsequent arrays in sequential order. The wells in the first array each have a size that permits entry of only one target entity into the well and each well in the first array has approximately the same size.

公开(公告)号：US20140057289A1

公开(公告)日：2014-02-27

申请号：US14037476

申请日：2013-09-26

Applicant: Purdue Research Foundation

Inventor： Cagri A. Savran ,  Philip S. Low ,  Chun-Li Chang ,  Wanfeng Huang

IPC: G01N27/74

CPC classification number: B01L3/502761 ,  B01L3/50273 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0877 ,  B01L2400/043 ,  G01N27/745 ,  G01N33/54326 ,  G01N33/574 ,  G01N33/57434 ,  G01N33/57492 ,  G01N2001/4016

Abstract: A microfluidic detection system for micrometer-sized entities, such as biological cells, includes a detector component incorporating a plate with a plurality of opening, the plate separating two chambers, one in communication with a fluid source containing target entities bound to magnetic beads. The openings are sized to always permit passage of the magnetic beads therethrough into a lower one of the chambers and are further sized to always prevent passage of the target entities from the upper one of the chambers. The detector component further includes a magnet positioned to pull unbound magnetic beads through the openings and to capture target entities bound to magnetic beads on the surface of the plate. In a further feature, the microfluidic detection system is configured to pass target molecules through the plate to be bound to a functionalized surface of the lower chamber.

Abstract translation: 用于微米尺寸实体（例如生物细胞）的微流体检测系统包括结合有多个开口的板的检测器部件，该板分离两个室，一个与包含与磁珠结合的目标实体的流体源连通。 这些开口的尺寸设置成总是允许磁珠通过其进入下部的一个室，并且进一步的尺寸以防止目标实体从上部的一个室中通过。 检测器部件还包括磁铁，该磁体定位成将未结合的磁珠拉过开口并且捕获与板的表面上的磁珠接合的目标实体。 在另一个特征中，微流体检测系统被配置成使目标分子穿过板结合到下腔室的官能化表面。

公开(公告)号：US20190291109A1

公开(公告)日：2019-09-26

申请号：US16275912

申请日：2019-02-14

Applicant: Purdue Research Foundation

Inventor： Cagri A. Savran ,  Philip S. Low ,  Chun-Li Chang ,  Wanfeng Huang

IPC: B01L3/00 ,  G01N33/574 ,  G01N33/543 ,  G01N27/74

Abstract: A microfluidic detection system for micrometer-sized entities, such as biological cells, includes a detector component incorporating a plate with a plurality of opening, the plate separating two chambers, one in communication with a fluid source containing target entities bound to magnetic beads. The openings are sized to always permit passage of the magnetic beads therethrough into a lower one of the chambers and are further sized to always prevent passage of the target entities from the upper one of the chambers. The detector component further includes a magnet positioned to pull unbound magnetic beads through the openings and to capture target entities bound to magnetic beads on the surface of the plate. In a further feature, the microfluidic detection system is configured to pass target molecules through the plate to be bound to a functionalized surface of the lower chamber.

公开(公告)号：US10207267B2

公开(公告)日：2019-02-19

申请号：US15355792

申请日：2016-11-18

Applicant: Purdue Research Foundation

Inventor： Cagri A. Savran ,  Philip S. Low ,  Chun-Li Chang ,  Wanfeng Huang

IPC: G01N33/553 ,  B01L3/00 ,  G01N33/543 ,  G01N27/74 ,  G01N33/574 ,  G01N1/40

Abstract: A microfluidic detection system for micrometer-sized entities, such as biological cells, includes a detector component incorporating a plate with a plurality of opening, the plate separating two chambers, one in communication with a fluid source containing target entities bound to magnetic beads. The openings are sized to always permit passage of the magnetic beads therethrough into a lower one of the chambers and are further sized to always prevent passage of the target entities from the upper one of the chambers. The detector component further includes a magnet positioned to pull unbound magnetic beads through the openings and to capture target entities bound to magnetic beads on the surface of the plate. In a further feature, the microfluidic detection system is configured to pass target molecules through the plate to be bound to a functionalized surface of the lower chamber.

公开(公告)号：US20170131283A1

公开(公告)日：2017-05-11

申请号：US15355792

申请日：2016-11-18

Applicant: Purdue Research Foundation

Inventor： Cagri A. Savran ,  Philip S. Low ,  Chun-Li Chang ,  Wanfeng Huang

IPC: G01N33/574 ,  G01N33/543 ,  G01N27/74

CPC classification number: B01L3/502761 ,  B01L3/50273 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0877 ,  B01L2400/043 ,  G01N27/745 ,  G01N33/54326 ,  G01N33/574 ,  G01N33/57434 ,  G01N33/57492 ,  G01N2001/4016

Abstract: A microfluidic detection system for micrometer-sized entities, such as biological cells, includes a detector component incorporating a plate with a plurality of opening, the plate separating two chambers, one in communication with a fluid source containing target entities bound to magnetic beads. The openings are sized to always permit passage of the magnetic beads therethrough into a lower one of the chambers and are further sized to always prevent passage of the target entities from the upper one of the chambers. The detector component further includes a magnet positioned to pull unbound magnetic beads through the openings and to capture target entities bound to magnetic beads on the surface of the plate. In a further feature, the microfluidic detection system is configured to pass target molecules through the plate to be bound to a functionalized surface of the lower chamber.

公开(公告)号：US20220055034A1

公开(公告)日：2022-02-24

申请号：US17512282

申请日：2021-10-27

Applicant: Purdue Research Foundation

Inventor： Cagri A. Savran ,  Chun-Li Chang ,  Wanfeng Huang ,  Onur Gur ,  Rohil Jain

IPC: B01L3/00 ,  B03C1/01 ,  B03C1/033 ,  B03C1/28

Abstract: Microfluidic systems and methods are described for capturing magnetic target entities bound to one or more magnetic beads. The systems include a well array device that includes a substrate with a surface that has a plurality of wells arranged in one or more arrays on the surface. A first array of wells is arranged adjacent to a first location on the surface. A second and subsequent arrays, if present, are arranged sequentially on the surface at second and subsequent locations. When a liquid sample is added onto the substrate and caused to flow, the liquid sample will flow across the first array first and then flow across the second and subsequent arrays in sequential order. The wells in the first array each have a size that permits entry of only one target entity into the well and each well in the first array has approximately the same size.

公开(公告)号：US11077439B2

公开(公告)日：2021-08-03

申请号：US16275912

申请日：2019-02-14

Applicant: Purdue Research Foundation

Inventor： Cagri A. Savran ,  Philip S. Low ,  Chun-Li Chang ,  Wanfeng Huang

IPC: G01N33/553 ,  B01L3/00 ,  G01N33/574 ,  G01N33/543 ,  G01N27/74 ,  G01N1/40

Abstract: A microfluidic detection system for micrometer-sized entities, such as biological cells, includes a detector component incorporating a plate with a plurality of opening, the plate separating two chambers, one in communication with a fluid source containing target entities bound to magnetic beads. The openings are sized to always permit passage of the magnetic beads therethrough into a lower one of the chambers and are further sized to always prevent passage of the target entities from the upper one of the chambers. The detector component further includes a magnet positioned to pull unbound magnetic beads through the openings and to capture target entities bound to magnetic beads on the surface of the plate. In a further feature, the microfluidic detection system is configured to pass target molecules through the plate to be bound to a functionalized surface of the lower chamber.

公开(公告)号：US20180348213A1

公开(公告)日：2018-12-06

申请号：US15761701

申请日：2016-09-22

Applicant: Purdue Research Foundation

Inventor： Cagri A. Savran ,  Chun-Li Chang ,  Wanfeng Huang

IPC: G01N33/543 ,  G01N1/38 ,  G01N1/40 ,  G01N33/569

CPC classification number: G01N33/56966 ,  G01N33/54326

Abstract: Additive techniques, including a direct-dilution method and a direct-incubation method, are described for isolating target entities in a fluid sample. In some implementations, a volume of a diluent is added to a fluid sample to generate a first mixture. The volume of the diluent added may be sufficient to obtain a specified viscosity of the first mixture lower than a viscosity of the fluid sample. A number of binding moiety-conjugated magnetic beads are added to the first mixture to generate a second mixture. The second mixture is incubated for a time that is sufficient for the binding moiety-conjugated magnetic beads to bind to rare target entities in the second mixture. A portion of the second mixture is injected into a fluidic chamber. A magnetic force is applied to attract the magnetized rare target entities in the second mixture to an isolation surface within the fluidic chamber.