公开(公告)号：US09340801B2

公开(公告)日：2016-05-17

申请号：US14390350

申请日：2013-04-02

Applicant: REG Life Sciences, LLC

Inventor： Derek L. Greenfield ,  Elizabeth J. Clarke ,  Eli S. Groban ,  Vikranth Arlagadda ,  Sungwon Lee ,  Xuezhi Li ,  Zhihao Hu ,  Baolong Zhu

IPC: C12N9/02 ,  C12P7/04 ,  C12P7/64

CPC classification number: C12N9/0008 ,  C12P7/04 ,  C12P7/6409 ,  C12Y102/99006 ,  Y02P20/52

Abstract: The disclosure relates to variant carboxylic acid reductase (CAR) enzymes for the improved production of fatty alcohols in recombinant host cells.

Abstract translation: 本公开涉及用于改善重组宿主细胞中脂肪醇生产的变体羧酸还原酶（CAR）酶。

公开(公告)号：US10676763B2

公开(公告)日：2020-06-09

申请号：US15326844

申请日：2015-07-17

Applicant: REG Life Sciences, LLC

Inventor： Andreas W. Schirmer ,  Noah Helman ,  Haibo Wang ,  Zhihao Hu ,  Vikranth Arlagadda ,  Alma Itzel Ramos-Solis ,  Elizabeth Clarke

IPC: C12P7/18 ,  C12N9/16 ,  C12N9/00 ,  C12N15/52 ,  C12N9/18 ,  C12N9/02 ,  C12N9/04 ,  C12N15/63

Abstract: The disclosure relates to fatty diols and recombinant microorganisms for producing them. More particularly, the disclosure relates to recombinant microorganisms engineered to produce fatty diols via fermentation. Further encompassed is a process that uses the microorganisms to produce fatty diols from a simple carbon source.

公开(公告)号：US20180135028A1

公开(公告)日：2018-05-17

申请号：US15695205

申请日：2017-09-05

Applicant: Reg Life Sciences, LLC

Inventor： Derek L. GREENFIELD ,  Elizabeth J. CLARKE ,  Eli S. GROBAN ,  Vikranth Arlagadda ,  Sungwon LEE ,  Xuezhi LI ,  Zhihao Hu ,  Baolong ZHU

IPC: C12N9/02 ,  C12P7/04 ,  C12P7/64

CPC classification number: C12N9/0008 ,  C12P7/04 ,  C12P7/6409 ,  C12Y102/99006 ,  Y02P20/52

Abstract: The disclosure relates to variant carboxylic acid reductase (CAR) enzymes for the improved production of fatty alcohols in recombinant host cells.

公开(公告)号：US09890401B2

公开(公告)日：2018-02-13

申请号：US14720240

申请日：2015-05-22

Applicant: REG Life Sciences, LLC

Inventor： Zhihao Hu ,  Vikranth Arlagadda

IPC: C12N1/20 ,  C12N9/02 ,  C12P7/64 ,  C12P7/04 ,  A61K38/00 ,  A61K31/045 ,  C12R1/01

CPC classification number: C12P7/64 ,  A61K31/045 ,  C12N1/20 ,  C12N9/0004 ,  C12N2501/71 ,  C12N2502/99 ,  C12P7/04 ,  C12R1/01 ,  Y02A50/473

Abstract: Methods and compositions, including nucleotide sequences, amino acid sequences, and host cells, for producing fatty alcohols are described.

公开(公告)号：US20180010137A1

公开(公告)日：2018-01-11

申请号：US15650285

申请日：2017-07-14

Applicant: REG Life Sciences, LLC

Inventor： Derek L. GREENFIELD ,  Andreas W. Schirmer ,  Elizabeth J. CLARKE ,  Eli S. GROBAN ,  Bernardo M. Da Costa ,  Zhihao Hu ,  Kevin Holden ,  Noah Helman

IPC: C12N15/70 ,  C12P7/64 ,  C12P7/04 ,  C12N9/02 ,  C12N9/90 ,  C12N9/88 ,  C12N9/12 ,  C12N9/10 ,  C12N9/04 ,  C12N9/00

CPC classification number: C12N15/70 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1288 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12P7/04 ,  C12P7/6409 ,  C12P7/6436 ,  C12Y101/011 ,  C12Y103/01009 ,  C12Y103/0101 ,  C12Y203/01039 ,  C12Y203/01041 ,  C12Y203/01179 ,  C12Y203/0118 ,  C12Y207/08007 ,  C12Y402/01059 ,  C12Y503/03014 ,  C12Y604/01002

Abstract: The invention relates to compositions and methods, including polynucleotide sequences, amino acid sequences, recombinant host cells and recombinant host cell cultures engineered to produce fatty acid derivative compositions comprising fatty acids, fatty alcohols, fatty aldehydes, fatty esters, alkanes, terminal olefins, internal olefins or ketones. The fatty acid derivative composition is produced extracellularly with a higher titer, yield or productivity than the corresponding wild type or non-engineered host cell.

公开(公告)号：US09587231B2

公开(公告)日：2017-03-07

申请号：US14826657

申请日：2015-08-14

Applicant: REG Life Sciences, LLC

Inventor： Louis Hom ,  Na Trinh ,  Murtaza Alibhai ,  Zhihao Hu ,  Eli Groban ,  Vikranth Arlagadda ,  Elizabeth Clarke

IPC: C12N9/16 ,  C12N15/00 ,  C12N1/20 ,  C07H21/04 ,  C12N9/18 ,  C10L1/02 ,  C12N15/63

CPC classification number: C12N9/18 ,  C07H21/04 ,  C10L1/02 ,  C12N9/16 ,  C12N15/00 ,  C12N15/63 ,  C12P21/06

Abstract: The present invention relates to novel mutant thioesterase enzymes and naturally-occurring equivalents thereof, compositions made from such enzymes and uses of thioesterase enzymes. In particular, the present invention provides mutant thioesterase enzymes that have altered properties, for example, altered substrate specificity, altered activity, altered selectivity, and/or altered proportional yields in the product mixtures. The present invention also provides polynucleotides encoding such mutant thioesterase enzymes, and vectors and host cells comprising such polynucleotides. The invention further provides for novel uses of thioesterases in the production of various fatty acid derivatives, which are useful as, or as components of, industrial chemicals and fuels.

Abstract translation: 本发明涉及新的突变硫酯酶及其天然存在的等同物，由这些酶制成的组合物和硫酯酶的用途。 特别地，本发明提供突变体硫酯酶，其具有改变的性质，例如改变的底物特异性，改变的活性，改变的选择性和/或在产物混合物中改变的比例产率。 本发明还提供了编码这种突变体硫酯酶的多核苷酸，以及包含这种多核苷酸的载体和宿主细胞。 本发明进一步提供硫酯酶在生产各种脂肪酸衍生物中的新用途，其可用作或作为工业化学品和燃料的组分。

公开(公告)号：US10301603B2

公开(公告)日：2019-05-28

申请号：US15695205

申请日：2017-09-05

Applicant: Reg Life Sciences, LLC

Inventor： Derek L. Greenfield ,  Elizabeth J. Clarke ,  Eli S. Groban ,  Vikranth Arlagadda ,  Sungwon Lee ,  Xuezhi Li ,  Zhihao Hu ,  Baolong Zhu

IPC: C12N9/06 ,  C12N9/02 ,  C12P7/04 ,  C12P7/64

Abstract: The disclosure relates to variant carboxylic acid reductase (CAR) enzymes for the improved production of fatty alcohols in recombinant host cells.

公开(公告)号：US20190031594A1

公开(公告)日：2019-01-31

申请号：US15954451

申请日：2018-04-16

Applicant: REG Life Sciences, LLC

Inventor： Zhihao Hu ,  Fernando Valle

IPC: C07C69/533 ,  C12N1/12 ,  C10L1/02 ,  C12N1/16 ,  C12N1/20 ,  C07C69/52 ,  C07C69/34 ,  C12N9/10 ,  C12N9/02 ,  C12N9/16 ,  C12P7/64 ,  C12N9/00 ,  C11C3/00 ,  C10L1/19 ,  C12N1/14

Abstract: Genetically engineered cells and microorganisms are provided that produce products from the fatty acid biosynthetic pathway (fatty acid derivatives), as well as methods of their use. The products are particularly useful as biofuels.

公开(公告)号：US09598706B2

公开(公告)日：2017-03-21

申请号：US13870426

申请日：2013-04-25

Applicant: REG Life Sciences, LLC

Inventor： Jay D. Keasling ,  Zhihao Hu ,  Chris Somerville ,  George Church ,  David Berry ,  Lisa C. Friedman ,  Andreas Schirmer ,  Shane Brubaker ,  Stephen B. Del Cardayre

IPC: C12P7/04 ,  C10L1/02 ,  C10L1/32 ,  C12P7/64 ,  C07C31/125 ,  C07C33/02

CPC classification number: C12P7/04 ,  C07C31/125 ,  C07C33/02 ,  C10L1/026 ,  C10L1/328 ,  C12P7/6436 ,  C12P7/6463 ,  C12P7/649 ,  Y02E50/13

Abstract: Compositions and methods for production of fatty alcohols using recombinant microorganisms are provided as well as fatty alcohol compositions produced by such methods.

公开(公告)号：US20160326499A1

公开(公告)日：2016-11-10

申请号：US15154636

申请日：2016-05-13

Applicant: Reg Life Sciences, LLC

Inventor： Derek L. GREENFIELD ,  Elizabeth CLARKE ,  Eli GROBAN ,  Vikranth Arlagadda ,  Sungwon LEE ,  Xuezhi LI ,  Zhihao Hu ,  Baolong ZHU

IPC: C12N9/02 ,  C12P7/04

CPC classification number: C12N9/0008 ,  C12P7/04 ,  C12P7/6409 ,  C12Y102/99006 ,  Y02P20/52

Abstract: The disclosure relates to variant carboxylic acid reductase (CAR) enzymes for the improved production of fatty alcohols in recombinant host cells.