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公开(公告)号：US20170369526A1
公开(公告)日：2017-12-28
申请号：US15533652
申请日：2015-12-11
Applicant: Rensselaer Polytechnic Institute
Inventor： Timothy Paul Cleland , Deepak Vashishth
Abstract: Embodiments of the invention relate generally to protein extraction and, more generally, to bone protein extraction methods that do not require demineralization. In one embodiment, the invention provides a method comprising: mixing a bone sample and a quantity of an extraction buffer comprising: ammonium phosphate dibasic; or ammonium phosphate dibasic and ammonium bicarbonate; or ammonium phosphate dibasic, ammonium bicarbonate, and guanidine HCl; or sodium phosphate dibasic and sodium bicarbonate; or sodium phosphate dibasic, sodium bicarbonate, and guanidine HCl; or potassium phosphate dibasic and potassium bicarbonate; or potassium phosphate dibasic, potassium bicarbonate, and guanidine HCl; and incubating the bone sample/extraction buffer mixture.
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Patent Agency Ranking
公开(公告)号：US11319342B2
公开(公告)日：2022-05-03
申请号：US15533652
申请日：2015-12-11
Applicant: Rensselaer Polytechnic Institute
Inventor： Timothy Paul Cleland , Deepak Vashishth
Abstract: Embodiments of the invention relate generally to protein extraction and, more generally, to bone protein extraction methods that do not require demineralization. In one embodiment, the invention provides a method comprising: mixing a bone sample and a quantity of an extraction buffer comprising: ammonium phosphate dibasic; or ammonium phosphate dibasic and ammonium bicarbonate; or ammonium phosphate dibasic, ammonium bicarbonate, and guanidine HCl; or sodium phosphate dibasic and sodium bicarbonate; or sodium phosphate dibasic, sodium bicarbonate, and guanidine HCl; or potassium phosphate dibasic and potassium bicarbonate; or potassium phosphate dibasic, potassium bicarbonate, and guanidine HCl; and incubating the bone sample/extraction buffer mixture.
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公开(公告)号：US20240047071A1
公开(公告)日：2024-02-08
申请号：US18216679
申请日：2023-06-30
Applicant: Rensselaer Polytechnic Institute
Inventor： Deepak Vashishth , Bowen Wang
CPC classification number: G16H50/30 , A61B5/7275 , A61B5/4504 , A61B5/14532
Abstract: A method of predicting bone fracture risk in a type 2 diabetes (“T2D”) patient includes: obtaining a plurality of averaged glycated hemoglobin (“HbA1c”) values for a plurality of T2D subjects over an observational period; binning the plurality of averaged HbA1c values into two predetermined categories; obtaining a plurality of total fracture incidences for the plurality of T2D subjects over a follow-up period; performing a plurality of linear regression model correlations between the binned HbA1c values and the plurality of total fracture incidences to determine a bone fracture rate prediction model; obtaining a measurement of an HbA1c value of the T2D patient; and analyzing the HbA1c value of the T2D patient with the bone fracture rate prediction model to determine the T2D patient's risk of bone fracture for a future period.
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公开(公告)号：US20190194247A1
公开(公告)日：2019-06-27
申请号：US16289782
申请日：2019-03-01
Applicant: Rensselaer Polytechnic Institute
Inventor： Timothy Paul Cleland , Deepak Vashishth
Abstract: Cell culture devices, and related methods and kits, for modeling isotropic-to-anisotropic cellular transitions are provided. The devices can include a substrate having an isotropic film surface with one or more regions of aligned fibers dispersed thereon.
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公开(公告)号：US12274809B2
公开(公告)日：2025-04-15
申请号：US15570942
申请日：2016-05-02
Applicant: RENSSELAER POLYTECHNIC INSTITUTE
Abstract: Provided is a method of mineralizing tissue, including placing within a body of a subject a substance, wherein the substance includes osteopontin and osteocalcin. Also provided is a tissue mineralization-promoting substance including osteopontin and osteocalcin, and an article for promoting tissue mineralization, including an implant which may be an orthopedic implant or an endosseous implant, and a substance disposed on a surface of the implant including osteopontin, and osteocalcin. A method of making a tissue mineralization-promoting substance, including combining osteopontin and osteocalcin, is also provided. Other features, including calcium phosphate, type-I collagen, mesenchymal stem cells, and growth factors, are also provided.
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公开(公告)号：US11685763B2
公开(公告)日：2023-06-27
申请号：US16289782
申请日：2019-03-01
Applicant: Rensselaer Polytechnic Institute
Inventor： Timothy Paul Cleland , Deepak Vashishth
Abstract: Embodiments of the invention relate generally to protein extraction and, more generally, to bone protein extraction methods that do not require demineralization. In one embodiment, the invention provides a method comprising: mixing a bone sample and a quantity of an extraction buffer comprising: ammonium phosphate dibasic; or ammonium phosphate dibasic and ammonium bicarbonate; or ammonium phosphate dibasic, ammonium bicarbonate, and guanidine HCl; or sodium phosphate dibasic and sodium bicarbonate; or sodium phosphate dibasic, sodium bicarbonate, and guanidine HCl; or potassium phosphate dibasic and potassium bicarbonate; or potassium phosphate dibasic, potassium bicarbonate, and guanidine HCl; and incubating the bone sample/extraction buffer mixture.
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