公开(公告)号：US11668024B2

公开(公告)日：2023-06-06

申请号：US16740106

申请日：2020-01-10

Applicant: SPIBER INC.

Inventor： Kana Ishida ,  Hironori Yamamoto ,  Hiroaki Suzumura ,  Kazuhide Sekiyama

IPC: D01F4/02 ,  D01C3/00 ,  C07K14/435 ,  D01F1/02

CPC classification number: D01F4/02 ,  C07K14/435 ,  C07K14/43518 ,  D01C3/00 ,  D01F1/02

Abstract: A polar solvent solution of the present invention is a polar solvent solution in which a solute containing a polyamine acid is dissolved in a polar solvent. The solution has a moisture content of less than 5 mass % based on 100 mass % of the solution. A method for producing a polar solvent solution of the present invention includes changing a moisture content of the solution to adjust the viscosity of the solution. Further, another method for producing a polar solvent solution includes reducing a moisture content of the solution to increase the viscosity of the solution. Thus, the present invention provides a polar solvent solution that enables stable spinning and casting without lowering its viscosity when used as dopes for spinning, film, etc., and methods for producing the same.

公开(公告)号：US20190031842A1

公开(公告)日：2019-01-31

申请号：US16073069

申请日：2017-01-27

Applicant: Riken ,  Spiber Inc. ,  Kojima Industries Corporation

Inventor： Keiji Numata ,  Kana Ishida ,  Hironori Yamamoto

IPC: C08J5/18 ,  C07K14/435

Abstract: The present invention provides, in one aspect, a method for producing a molded article, the method comprising exposing a molded article precursor comprising a protein to an environment with a relative humidity of 80% or more to obtain the molded article.

公开(公告)号：US20210292752A1

公开(公告)日：2021-09-23

申请号：US17266566

申请日：2019-08-13

Applicant: Spiber Inc. ,  The University of Tokyo

Inventor： Kana Ishida ,  Soh Ishiguro ,  Nozomu Yachie ,  Chikako Sato ,  Junichi Sugahara

IPC: C12N15/10

Abstract: Disclosed is a method for isolating or identifying target clone cells from a cell population, the method including steps of: preparing a cell population into which a barcode sequence and at least one reporter protein abnormal expression cassette linked to the barcode sequence are introduced; introducing a barcode sequence recognition module targeting an arbitrary barcode sequence and a nucleic acid mutation repair enzyme into cells; repairing a nucleic acid mutation causing abnormal expression occurring in the at least one reporter protein abnormal expression cassette by expression of a complex of the barcode sequence recognition module and the nucleic acid mutation repair enzyme in a cell containing the target barcode sequence, to induce normal expression of the reporter protein; and isolating or identifying target clone cells in which the reporter protein is expressed.

公开(公告)号：US10975206B2

公开(公告)日：2021-04-13

申请号：US15564373

申请日：2016-04-04

Applicant: SPIBER INC.

Inventor： Kana Ishida ,  Hironori Yamamoto ,  Hiroaki Suzumura ,  Kazuhide Sekiyama

IPC: C08J3/09 ,  C07K1/14 ,  C07K14/435 ,  C08L89/00 ,  D01F1/10 ,  D01F4/02 ,  C07K14/00

Abstract: A polar solvent solution of the present invention is a polar solvent solution in which a solute containing a polyamine acid is dissolved in a polar solvent. An inorganic salt is added to the solution, and a mole ratio of moisture to the inorganic salt in the solution is 2.5×m×n or less, where m represents the number of cations forming the inorganic salt and n represents a charge number of the cation. A production method of the present invention is a method for producing the above solution, including: changing at, least one of a moisture content and a content of the inorganic salt in the solution to adjust a viscosity of the solution. Thus, the present invention provides a polar solvent solution whose viscosity can be easily adjusted to a desired value and thus enables stable spinning and casting when used as dopes for spinning, film, etc., and methods for producing the same.

公开(公告)号：US20190031843A1

公开(公告)日：2019-01-31

申请号：US16073103

申请日：2017-01-27

Applicant: Riken ,  Spiber Inc.

Inventor： Keiji Numata ,  Kana Ishida ,  Hironori Yamamoto

IPC: C08J5/18 ,  C07K14/435 ,  D01F4/02 ,  B29C39/00 ,  D01D10/00 ,  B29C39/02 ,  B29C71/00

Abstract: The present invention provides, in one aspect, a method for producing a molded article, the method comprising exposing a molded article precursor comprising a protein to an environment with a relative humidity of 90% or more to obtain the molded article.