公开(公告)号：US10065997B2

公开(公告)日：2018-09-04

申请号：US14764463

申请日：2014-04-18

Applicant: SPIBER INC.

Inventor： Toshiaki Osawa ,  Keisuke Morita

IPC: C07K14/43 ,  A61K8/64 ,  A61K47/42 ,  C08J9/28 ,  C08L89/00 ,  A61K8/42 ,  A61K8/46 ,  A61Q19/00 ,  B01J20/24 ,  C11B9/00 ,  A61K9/19 ,  C07K14/435 ,  A61K8/02 ,  A61K8/98

CPC classification number: C07K14/43518 ,  A61K8/0279 ,  A61K8/42 ,  A61K8/46 ,  A61K8/64 ,  A61K8/987 ,  A61K9/19 ,  A61K47/42 ,  A61K2800/10 ,  A61K2800/56 ,  A61K2800/84 ,  A61Q19/00 ,  B01J20/24 ,  C08J9/28 ,  C08J2201/0484 ,  C08J2201/0544 ,  C08J2205/022 ,  C08J2205/028 ,  C08J2389/00 ,  C08L89/00 ,  C11B9/00

Abstract: A polypeptide porous body of the present invention is a porous body of a polypeptide derived from spider silk proteins. The polypeptide includes a water-insoluble polypeptide. The polypeptide porous body has an apparent density of 0.1 g/cm3 or less. A method for producing the polypeptide porous body includes: a solution production step in which the polypeptide is dissolved in at least one solvent selected from DMSO, DMF, and these with an inorganic salt, so as to obtain a solution of the polypeptide; a step in which the solution produced in the solution production step is substituted with a water-soluble solvent so as to obtain a polypeptide gel; and a step in which the polypeptide gel is dried. Thereby, the present invention provides a polypeptide porous body having excellent water absorbability and a polypeptide porous body suitable for application to a living body, and a method for producing the same.

公开(公告)号：US10899792B2

公开(公告)日：2021-01-26

申请号：US16324280

申请日：2017-08-10

Applicant: Spiber Inc.

Inventor： Toshiaki Osawa ,  Yuya Sato ,  Keisuke Morita

IPC: C07K1/34 ,  C07K1/30 ,  C12P21/02 ,  C12P21/00 ,  C07K1/14

Abstract: An object of the present invention is to provide a method for efficiently separating insoluble bodies of a recombinant protein from a recombinant cell expressing a target recombinant protein as insoluble bodies in the cell. The present invention provides a method for producing a recombinant protein aggregate by separating insoluble bodies of a recombinant protein from a recombinant cell expressing the recombinant protein as insoluble bodies in the cell, including disrupting the recombinant cell, aggregating the insoluble bodies of the recombinant protein, and separating the resulting aggregate.

公开(公告)号：US09732125B2

公开(公告)日：2017-08-15

申请号：US14764472

申请日：2014-04-18

Applicant: SPIBER INC.

Inventor： Toshiaki Osawa ,  Keisuke Morita

IPC: C07K14/435 ,  A61K8/64 ,  A61K8/42 ,  A61K8/46 ,  A61Q19/00 ,  A61K8/02 ,  C08J3/12 ,  C08L89/00 ,  C09D7/12 ,  C08J3/05 ,  C08J3/07 ,  A61K47/42 ,  A61K9/51

CPC classification number: C07K14/43518 ,  A61K8/0279 ,  A61K8/42 ,  A61K8/46 ,  A61K8/64 ,  A61K9/5169 ,  A61K47/42 ,  A61K2800/412 ,  A61K2800/56 ,  A61K2800/84 ,  A61Q19/00 ,  C08J3/05 ,  C08J3/07 ,  C08J3/12 ,  C08J2389/00 ,  C08L89/00 ,  C09D7/65 ,  C09D7/68

Abstract: Polypeptide particles of the present invention are particles of a polypeptide derived from spider silk proteins, and have an average particle size of 1000 nm or less. A method for producing polypeptide particles of the present invention includes: a solution production step in which the polypeptide is dissolved in at least one solvent selected from the group consisting of DMSO, DMF, and these with an inorganic salt, so as to obtain a solution of the polypeptide; a step in which the solution produced in the solution production step is substituted with a water-soluble solvent so as to obtain an aqueous solution of the polypeptide; and a step in which the aqueous solution of the polypeptide is dried. Thereby, the present invention provides polypeptide particles suitable for application to a living body and capable of being applied to cosmetics, etc., while identifying the properties of the polypeptide particles, and a method for producing the same.

公开(公告)号：US20190177363A1

公开(公告)日：2019-06-13

申请号：US16324280

申请日：2017-08-10

Applicant: Spiber Inc.

Inventor： Toshiaki Osawa ,  Yuya Sato ,  Keisuke Morita

IPC: C07K1/34 ,  C07K1/30 ,  C12P21/02

Abstract: An object of the present invention is to provide a method for efficiently separating insoluble bodies of a recombinant protein from a recombinant cell expressing a target recombinant protein as insoluble bodies in the cell. The present invention provides a method for producing a recombinant protein aggregate by separating insoluble bodies of a recombinant protein from a recombinant cell expressing the recombinant protein as insoluble bodies in the cell, including disrupting the recombinant cell, aggregating the insoluble bodies of the recombinant protein, and separating the resulting aggregate.

公开(公告)号：US09968682B2

公开(公告)日：2018-05-15

申请号：US14764455

申请日：2014-04-18

Applicant: SPIBER INC.

Inventor： Toshiaki Osawa ,  Keisuke Morita

IPC: A61K9/08 ,  A61K38/17 ,  A61L27/22 ,  A61L27/52 ,  A61K47/42 ,  A61L26/00 ,  A61L31/14 ,  A61K9/00 ,  C07K14/435 ,  A61L27/36 ,  A61L27/60

CPC classification number: A61K47/42 ,  A61K9/0087 ,  A61K38/1767 ,  A61L26/008 ,  A61L27/227 ,  A61L27/3604 ,  A61L27/3687 ,  A61L27/52 ,  A61L27/60 ,  A61L31/145 ,  A61L2430/06 ,  C07K14/43518

Abstract: A polypeptide hydrogel of the present invention is a hydrogel of a polypeptide derived from spider silk proteins, and has a moisture content of 85.0 to 99.9 mass % based on 100 mass % of the polypeptide hydrogel. A method for producing the hydrogel includes: a solution production step in which a polypeptide derived from spider silk proteins is dissolved in at least one dissolving solvent selected from the group consisting of: (A) dimethyl sulfoxide; (B) dimethyl sulfoxide with an inorganic salt; and (C) N, N-dimethylformamide with an inorganic salt, so as to obtain a solution of the polypeptide; and a substitution step in which the solution produced in the solution production step is substituted with a water-soluble solvent. Thus, the present invention provides a polypeptide hydrogel having excellent water retentivity and a polypeptide hydrogel suitable for application to a living body.