公开(公告)号：US09588123B2

公开(公告)日：2017-03-07

申请号：US14683693

申请日：2015-04-10

Applicant: SurModics IVD, Inc.

Inventor： Sean Lundquist

IPC: G01N33/58 ,  C12Q1/26 ,  C12Q1/28

CPC classification number: G01N33/583 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q2326/10 ,  G01N33/581

Abstract: The disclosure is directed to compositions, kits, and methods for performing colorimetric analysis. A composition for a colorimetric assay can be prepared that includes an aromatic amine chromogenic substrate and a zwitterionic solubilization reagent that is N,N,N-trimethylglycine (betaine). The zwitterionic solubilization reagent is highly useful as a non-encapsulating solubilization reagent and provided faster kinetics and increased dye content of an aromatic amine chromogenic substrate in an enzymatic reaction. In turn, this can result in improvements in detection of an analyte in a sample and also can increase the sample throughput. The aromatic amine chromogenic substrate and N,N,N-trimethylglycine solubilization reagent can be used in assays such as ELISAs in order to provide a more accurate and faster detection of analytes in a biological sample.

Abstract translation: 本公开涉及用于进行比色分析的组合物，试剂盒和方法。 可以制备用于比色测定的组合物，其包括芳族胺显色底物和N，N，N-三甲基甘氨酸（甜菜碱）的两性离子溶解试剂。 两性离子溶解试剂作为非包封的增溶剂是非常有用的，并提供酶反应中芳族胺显色底物的更快的动力学和增加的染料含量。 反过来，这可以导致样品中分析物的检测的改进，并且还可以增加样品通量。 芳族胺显色底物和N，N，N-三甲基甘氨酸增溶剂可用于诸如ELISA的测定中，以便提供对生物样品中分析物的更准确和更快速的检测。

公开(公告)号：US20150293110A1

公开(公告)日：2015-10-15

申请号：US14683693

申请日：2015-04-10

Applicant: SurModics IVD, Inc.

Inventor： Sean Lundquist

IPC: G01N33/58

CPC classification number: G01N33/583 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q2326/10 ,  G01N33/581

Abstract: The disclosure is directed to compositions, kits, and methods for performing colorimetric analysis. A composition for a colorimetric assay can be prepared that includes an aromatic amine chromogenic substrate and a zwitterionic solubilization reagent that is N,N,N-trimethylglycine (betaine). The zwitterionic solubilization reagent is highly useful as a non-encapsulating solubilization reagent and provided faster kinetics and increased dye content of an aromatic amine chromogenic substrate in an enzymatic reaction. In turn, this can result in improvements in detection of an analyte in a sample and also can increase the sample throughput. The aromatic amine chromogenic substrate and N,N,N-trimethylglycine solubilization reagent can be used in assays such as ELISAs in order to provide a more accurate and faster detection of analytes in a biological sample.

Abstract translation: 本公开涉及用于进行比色分析的组合物，试剂盒和方法。 可以制备用于比色测定的组合物，其包括芳族胺显色底物和N，N，N-三甲基甘氨酸（甜菜碱）的两性离子溶解试剂。 两性离子溶解试剂作为非包封的增溶剂是非常有用的，并提供酶反应中芳族胺显色底物的更快的动力学和增加的染料含量。 反过来，这可以导致样品中分析物的检测的改进，并且还可以增加样品通量。 芳族胺显色底物和N，N，N-三甲基甘氨酸增溶剂可用于诸如ELISA的测定中，以便提供对生物样品中分析物的更准确和更快速的检测。