公开(公告)号：US09279806B2

公开(公告)日：2016-03-08

申请号：US14171957

申请日：2014-02-04

Applicant: THE HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Benzhong Tang ,  Yuning Hong ,  Sijie Chen ,  Tsz Kin Ryan Kwok ,  Wai Tung Leung

IPC: C12Q1/00 ,  G01N33/53 ,  G01N33/543 ,  G01N33/542 ,  G01N21/64 ,  G01N21/77

CPC classification number: G01N33/542 ,  G01N2021/6432 ,  G01N2021/7786 ,  G01N2333/4709 ,  G01N2333/62

Abstract: Compounds that exhibit aggregation induced emission (AIE), and more particularly to water-soluble conjugated polyene compounds that exhibit aggregation induced emission. The conjugated polyene compounds can be used as bioprobes for DNA detection, G-quadruplex identification, and potassium-ion sensing. The polyenes also can be utilized as an external fluorescent marker to study conformational structures, to monitor folding processes of label-free oligonucleotides with G-rich strand sequences, and to visualize DNA bands in PAGE assay. The polyenes have applications in high-throughput anticancer drug screening and are useful for the development of efficient anti-cancer drugs. Furthermore, the present subject matter can also be used to monitor fibrillation of amyloid proteins and to facilitate the storage and delivery thereof.

Abstract translation: 表现出聚集诱导发射（AIE）的化合物，更具体地说是表现出聚集诱导发射的水溶性共轭多烯化合物。 共轭多烯化合物可用作DNA检测，G-四链体鉴定和钾离子感测的生物探针。 多烯还可以用作外部荧光标记物来研究构象结构，监测具有富G链的序列的无标记寡核苷酸的折叠过程，并在PAGE测定中显现DNA条带。 多烯在高通量抗癌药物筛选中有应用，可用于开发有效的抗癌药物。 此外，本主题也可用于监测淀粉样蛋白的原纤维化并促进其储存和递送。

公开(公告)号：US09315465B2

公开(公告)日：2016-04-19

申请号：US14164591

申请日：2014-01-27

Applicant: THE HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Benzhong Tang ,  Yuning Hong ,  Sijie Chen ,  Wai Tung Leung ,  Engui Zhao ,  Haiqin Deng

IPC: G01N33/52 ,  C07D217/04 ,  C07F9/54 ,  C07D209/08 ,  A61K49/00

CPC classification number: C07D217/04 ,  A61K49/0023 ,  C07D209/08 ,  C07F9/5449 ,  G01N33/52

Abstract: AIE (aggregation-induced emission)-active TPE derivatives, TPE-TPP, TPE-MitoR and TPE-IQ are contemplated. These specific TPE derivatives are useful as fluorescent agents for mitochondrial imaging and as apoptosis inducers. Possessing high specificity to mitochondria, superior photostability and appreciable tolerance to microenvironment change, TPE derivatives are well-suited imaging agents for mitochondrial targeting and morphological change tracking. Because of their synthetic flexibility, TPE derivatives can be further modified as dual-functional probes for an array of applications such as sensing of ROS, metal ions, or pH change in mitochondria.

Abstract translation: 考虑了AIE（聚集诱发发射）活性TPE衍生物，TPE-TPP，TPE-MitoR和TPE-IQ。 这些特异性TPE衍生物可用作线粒体成像和凋亡诱导剂的荧光剂。 对线粒体具有高度的特异性，优异的光稳定性和对微环境变化的显着耐受性，TPE衍生物是用于线粒体靶向和形态学变化追踪的非常适合的成像剂。 由于其合成灵活性，TPE衍生物可进一步修改为用于应用阵列的双功能探针，例如感测ROS，金属离子或线粒体中的pH变化。

公开(公告)号：US09618453B2

公开(公告)日：2017-04-11

申请号：US13899070

申请日：2013-05-21

Applicant: THE HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Benzhong Tang ,  Ka Ming Ng ,  Qian Luo ,  Yong Yu ,  Yuning Hong ,  Jianzhao Liu ,  Sijie Chen ,  Wing Yip Lam ,  Zhengke Wang ,  Wei Qin ,  Tsz Kin Kwok

IPC: C12Q1/04 ,  G01N21/64 ,  B82Y15/00 ,  G01N1/30 ,  C07F7/08

CPC classification number: G01N21/6486 ,  B82Y15/00 ,  C07F7/0805 ,  C07F7/0816 ,  G01N1/30 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6439

Abstract: Provided herein are fluorescent bioprobes comprising fluorogens that exhibit aggregation-induced emission (AIE) labeled on biomolecules. The present subject matter relates to a fluorescent bioprobe comprising one or more fluorogen labeled on chitosan. The present subject matter is also directed to methods of preparing the fluorescent bioprobes, methods of labeling and detecting DNA and/or proteins with the fluorescent bioprobe, and methods of cell imaging including live cell tracking.

公开(公告)号：US10113968B2

公开(公告)日：2018-10-30

申请号：US15100707

申请日：2014-12-03

Applicant: The Hong Kong University of Science and Technology

Inventor： Benzhong Tang ,  Yuning Hong ,  Wai Tung Leung

IPC: G01N21/64 ,  G01N33/483

Abstract: The present subject matter relates to a one-step method of detecting and quantifying cardiolipin in a sample using a positively charged AIE luminogen by introducing the AIE luminogen to a solution containing the sample and measuring fluorescence intensity of the solution; a method of quantifying isolated mitochondria using a positively charged AIE luminogen by staining a sample containing isolated mitochondria with the AIE luminogen and measuring the fluorescence intensity; and a method of quantifying isolated mitochondria using a positively charged AIE luminogen by introducing the AIE luminogen to a sample containing isolated mitochondria, wherein the AIE luminogen stains the isolated mitochondria and identifying the stained isolated mitochondria under microscope. With improved sensitivity and excellent selectivity to CL over other major mitochondrial membrane lipids, an aggregation-induced emission-active fluorogen, TTAPE-Me, may serve as a valuable fluorescent sensor for CL detection and quantification and the quantification of isolated mitochondria.

公开(公告)号：US09409928B2

公开(公告)日：2016-08-09

申请号：US13649819

申请日：2012-10-11

Applicant: The Hong Kong University of Science and Technology

Inventor： Benzhong Tang ,  Ka Ming Ng ,  Qian Luo ,  Yong Yu ,  Yuning Hong ,  Jianzhao Liu ,  Sijie Chen ,  Wing Yip Lam

IPC: C12Q1/04 ,  C07F7/08 ,  B82Y15/00 ,  G01N21/64

CPC classification number: C07F7/081 ,  B82Y15/00 ,  G01N21/6458 ,  G01N2021/6439 ,  Y10T428/2982

Abstract: Fluorescent bioprobes comprising luminogen formed nanoparticles comprising luminogens with aggregation-induced emission (AIE) properties, which can be used for long-term cell tracking. The luminogens are nonemissive in organic solution but become highly emissive when aggregated in aqueous solution. The fluorescent molecules can readily pass through cell membranes, stain only the cell cytoplasm, and form highly emissive nanoaggregates in aqueous media without any obvious cytoxicity in the living cells. Furthermore, the molecules can be retained inside the cells without noticeable leakage to the outside. Therefore, these AIE-based compounds can be used as selective and cell-compatible fluroescent bioprobes for long-term live cell tracking and imaging.

Abstract translation: 包含发光体形成的纳米颗粒的荧光生物探针包含具有聚集诱发发射（AIE）性质的发光体，其可用于长期细胞跟踪。 发光体在有机溶液中是非发射的，但当在水溶液中聚集时变得高度发射。 荧光分子可以容易地通过细胞膜，仅染色细胞质，并且在水性介质中形成高度发射的纳米聚集体，而在活细胞中没有任何明显的细胞毒性。 此外，分子可以保留在细胞内，而没有明显的泄漏到外部。 因此，这些基于AIE的化合物可用作选择性和细胞相容性的荧光生物探针，用于长期活细胞跟踪和成像。