公开(公告)号：US20170220000A1

公开(公告)日：2017-08-03

申请号：US15500880

申请日：2015-07-31

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Alon Greenbaum ,  Yibo Zhang ,  Alborz Feizi ,  Wei Luo

IPC: G03H1/00 ,  G03H1/08 ,  H04N5/232 ,  G03H1/04 ,  G01N15/14 ,  H04N5/225

CPC classification number: G03H1/0005 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1475 ,  G01N2015/1006 ,  G01N2015/144 ,  G01N2015/1445 ,  G01N2015/1454 ,  G03H1/0443 ,  G03H1/0866 ,  G03H1/0891 ,  G03H2001/005 ,  G03H2001/0447 ,  G03H2001/0454 ,  G03H2001/046 ,  G03H2001/0816 ,  G03H2001/0883 ,  G03H2001/2655 ,  G03H2210/42 ,  G03H2226/02 ,  G03H2227/03 ,  H04N5/2256 ,  H04N5/23238

Abstract: A method for lens-free imaging of a sample or objects within the sample uses multi-height iterative phase retrieval and rotational field transformations to perform wide FOV imaging of pathology samples with clinically comparable image quality to a benchtop lens-based microscope. The solution of the transport-of-intensity (TIE) equation is used as an initial guess in the phase recovery process to speed the image recovery process. The holographically reconstructed image can be digitally focused at any depth within the object FOV (after image capture) without the need for any focus adjustment, and is also digitally corrected for artifacts arising from uncontrolled tilting and height variations between the sample and sensor planes. In an alternative embodiment, a synthetic aperture approach is used with multi-angle iterative phase retrieval to perform wide FOV imaging of pathology samples and increase the effective numerical aperture of the image.

公开(公告)号：US20210285864A1

公开(公告)日：2021-09-16

申请号：US16333139

申请日：2017-09-22

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Alborz Feizi ,  Alon Greenbaum

IPC: G01N15/14 ,  G06T7/11 ,  G06K9/00 ,  G06K9/62

Abstract: A lens-free microscope system for automatically analyzing yeast cell viability in a stained sample includes a portable, lens-free microscopy device that includes a housing containing a light source coupled to an optical fiber, the optical fiber spaced away several centimeters from an image sensor disposed at one end of the housing, wherein the stained sample is disposed on the image sensor or a sample holder adjacent to the image sensor. Hologram images are transferred to a computing device having image processing software contained therein, the image processing software identifying yeast cell candidates of interest from back-propagated images of the stained sample, whereby a plurality of spatial features are extracted from the yeast cell candidates of interest and subject to a trained machine learning model to classify the yeast cell candidates of interest as live or dead.

公开(公告)号：US11320362B2

公开(公告)日：2022-05-03

申请号：US16333139

申请日：2017-09-22

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Alborz Feizi ,  Alon Greenbaum

IPC: G06K9/00 ,  G01N15/14 ,  G06T7/11 ,  G06K9/62 ,  G01N15/10

Abstract: A lens-free microscope system for automatically analyzing yeast cell viability in a stained sample includes a portable, lens-free microscopy device that includes a housing containing a light source coupled to an optical fiber, the optical fiber spaced away several centimeters from an image sensor disposed at one end of the housing, wherein the stained sample is disposed on the image sensor or a sample holder adjacent to the image sensor. Hologram images are transferred to a computing device having image processing software contained therein, the image processing software identifying yeast cell candidates of interest from back-propagated images of the stained sample, whereby a plurality of spatial features are extracted from the yeast cell candidates of interest and subject to a trained machine learning model to classify the yeast cell candidates of interest as live or dead.

公开(公告)号：US20170357083A1

公开(公告)日：2017-12-14

申请号：US15624624

申请日：2017-06-15

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Alon Greenbaum

IPC: G02B21/36 ,  G06T3/00 ,  G03H1/08 ,  G03H1/00 ,  G03H1/04 ,  G03H1/26

CPC classification number: G02B21/365 ,  G02B21/367 ,  G03H1/0005 ,  G03H1/0443 ,  G03H1/0866 ,  G03H2001/005 ,  G03H2001/0447 ,  G03H2001/0454 ,  G03H2001/2655 ,  G03H2210/12 ,  G03H2210/55 ,  G03H2240/56 ,  G06T3/0068

Abstract: A method of imaging includes illuminating a sample spaced apart from an image sensor at a multiple distances. Image frames of the sample obtained at each distance are registered to one another and lost phase information from the registered higher resolution image frames is iteratively recovered. Amplitude and/or phase images of the sample are reconstructed based at least in part on the recovered lost phase information.