公开(公告)号：US11422503B2

公开(公告)日：2022-08-23

申请号：US16952492

申请日：2020-11-19

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Alon Grinbaum ,  Yibo Zhang ,  Alborz Feizi ,  Wei Luo

IPC: G03H1/00 ,  G03H1/04 ,  G01N15/14 ,  G03H1/08 ,  H04N5/225 ,  H04N5/232 ,  G01N15/10 ,  G03H1/26

Abstract: A method for lens-free imaging of a sample or objects within the sample uses multi-height iterative phase retrieval and rotational field transformations to perform wide FOV imaging of pathology samples with clinically comparable image quality to a benchtop lens-based microscope. The solution of the transport-of-intensity (TIE) equation is used as an initial guess in the phase recovery process to speed the image recovery process. The holographically reconstructed image can be digitally focused at any depth within the object FOV (after image capture) without the need for any focus adjustment, and is also digitally corrected for artifacts arising from uncontrolled tilting and height variations between the sample and sensor planes. In an alternative embodiment, a synthetic aperture approach is used with multi-angle iterative phase retrieval to perform wide FOV imaging of pathology samples and increase the effective numerical aperture of the image.

公开(公告)号：US20210181673A1

公开(公告)日：2021-06-17

申请号：US16952492

申请日：2020-11-19

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Alon Grinbaum ,  Yibo Zhang ,  Alborz Feizi ,  Wei Luo

IPC: G03H1/00 ,  G03H1/04 ,  G01N15/14 ,  G03H1/08 ,  H04N5/225 ,  H04N5/232

Abstract: A method for lens-free imaging of a sample or objects within the sample uses multi-height iterative phase retrieval and rotational field transformations to perform wide FOV imaging of pathology samples with clinically comparable image quality to a benchtop lens-based microscope. The solution of the transport-of-intensity (TIE) equation is used as an initial guess in the phase recovery process to speed the image recovery process. The holographically reconstructed image can be digitally focused at any depth within the object FOV (after image capture) without the need for any focus adjustment, and is also digitally corrected for artifacts arising from uncontrolled tilting and height variations between the sample and sensor planes. In an alternative embodiment, a synthetic aperture approach is used with multi-angle iterative phase retrieval to perform wide FOV imaging of pathology samples and increase the effective numerical aperture of the image.