公开(公告)号：US11542292B2

公开(公告)日：2023-01-03

申请号：US16349438

申请日：2017-11-09

Applicant: TOKYO UNIVERSITY OF SCIENCE FOUNDATION

Inventor： Takeshi Wada ,  Tatsuya Saito ,  Yuka Ishii ,  Yohei Nukaga

IPC: C07H19/073 ,  C07H19/173 ,  C07H21/00 ,  C07F5/02 ,  C07F9/6584 ,  C07F9/6558 ,  C12N15/113

Abstract: Provided a polymerizable compound represented by the following Formula A-1 or Formula A-2: In Formula A-1 or Formula A-2, R1 represents an electron-donating group; n represents an integer from 1 to 5; R2 represents a hydrogen atom, a halogen atom, or —ORO, wherein RO represents a hydrogen atom, an alkyl group, or a protecting group of a hydroxy group; R3 represents a hydrogen atom or a protecting group of a hydroxy group; and X represents a structure represented by any one of Formula B-1 to Formula B-5.

公开(公告)号：US20240262859A1

公开(公告)日：2024-08-08

申请号：US18566013

申请日：2022-06-02

Applicant: TOKYO UNIVERSITY OF SCIENCE FOUNDATION

Inventor： Takeshi Wada ,  Kazuki Sato ,  Yuhei Takahashi

IPC: C07H23/00 ,  C07H1/00

CPC classification number: C07H23/00 ,  C07H1/00

Abstract: A novel nucleic acid oligomer and a method for producing the same. The nucleic acid oligomer includes a nucleotide unit represented by general formula (1) and a nucleotide unit represented by general formula (2), and optionally includes a nucleotide unit represented by general formula (3). In general formulas (1), (2), and (3): R1 represents a hydrogen atom, a hydroxyl group, an alkoxy group, an alkenyloxy group, an acyloxy group, a trialkylsilyloxy group, an alkoxyalkoxy group, a haloalkoxyalkoxy group, or a halogenyl group, and R2 represents a hydrogen atom, or R1 and R2 bond together to form a five- or more-membered ring optionally having a substituent and optionally having a hetero atom; Bs represents a nucleobase selected from the group consisting of adenine, guanine, cytosine, 5-methylcytosine, thymine, and uracil, the nucleobase optionally having a nucleobase protecting group; and X+ represents a counter cation

公开(公告)号：US11932666B2

公开(公告)日：2024-03-19

申请号：US17965125

申请日：2022-10-13

Applicant: TOKYO UNIVERSITY OF SCIENCE FOUNDATION

Inventor： Takeshi Wada ,  Tatsuya Saito ,  Yuka Ishii ,  Yohei Nukaga

IPC: C07H19/073 ,  C07F5/02 ,  C07F9/6558 ,  C07F9/6584 ,  C07H19/173 ,  C07H21/00 ,  C12N15/113

CPC classification number: C07H19/173 ,  C07F5/02 ,  C07F9/6558 ,  C07F9/6584 ,  C07H19/073 ,  C07H21/00 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/31 ,  C12N2310/321 ,  Y02P20/55

Abstract: Provided is a polymerizable compound represented by a Formula E-1 or Formula E-2 shown in the description. In Formula E-1 or Formula E-2, R1 represents an alkoxy group, —NRN2, a hydroxy group, an aryl group, or an alkyl group, wherein RN each independently represents a hydrogen atom or an alkyl group having 1 to 10 carbon atoms; n represents an integer from 1 to 5; R3 represents a hydrogen atom, an acetyl group, a phenoxyacetyl group, a pivaloyl group, a benzyl group, a 4-methoxybenzyl group, a benzoyl group, a triphenylmethyl group, a 4,4′-dimethoxytrityl (DMTr) group, a 4-methoxytrityl (MMTr) group, a 9-phenylxanthenyl group, a trimethylsilyl group, a cyanomethoxymethyl group, a 2-(cyanoethoxy)ethyl group, or a cyanoethoxymethyl group; and X represents a structure represented by any one of Formula B-1 to Formula B-5 shown in the description.

公开(公告)号：US20190270765A1

公开(公告)日：2019-09-05

申请号：US16349438

申请日：2017-11-09

Applicant: TOKYO UNIVERSITY OF SCIENCE FOUNDATION

Inventor： Takeshi Wada ,  Tatsuya Saito ,  Yuka Ishii ,  Yohei Nukaga

IPC: C07H19/173 ,  C07H19/073 ,  C07H21/00 ,  C12N15/113

Abstract: Provided a polymerizable compound represented by the following Formula A-1 or Formula A-2: In Formula A-1 or Formula A-2, R1 represents an electron-donating group; n represents an integer from 1 to 5; R2 represents a hydrogen atom, a halogen atom, or —ORO, wherein RO represents a hydrogen atom, an alkyl group, or a protecting group of a hydroxy group; R3 represents a hydrogen atom or a protecting group of a hydroxy group; and X represents a structure represented by any one of Formula B-1 to Formula B-5.

公开(公告)号：US20190194746A1

公开(公告)日：2019-06-27

申请号：US16083347

申请日：2017-03-09

Applicant: TOKYO UNIVERSITY OF SCIENCE FOUNDATION

Inventor： Takeshi Wada ,  Reijiro Yoshino ,  Rintaro Hara ,  Yohei Nukaga

IPC: C12Q1/6876 ,  C12Q1/6853 ,  C07H21/04

CPC classification number: C12Q1/6876 ,  A61K31/7088 ,  A61K48/00 ,  C07H21/04 ,  C12N15/00 ,  C12N15/113 ,  C12Q1/6853 ,  C12Q2527/101 ,  C12Q2527/107 ,  C12Q2600/166

Abstract: A nucleic acid oligomer for RNA hybrid formation, including a structure formed of from 3 to 50 continuous units of at least one unit of a nucleotide unit represented by the following General Formula (1) or a nucleotide unit represented by the following General Formula (2); and having a base length of from 8 bases to 50 bases: in which in the formulae, R1 represents a hydrogen atom, an alkoxy group, an alkenyloxy group, an acyloxy group, a trialkylsilyloxy group, or a halogenyl group, and, in the formulae, each Bs1 independently represents a pyrimidine base that may have a protecting group, a hydrogen atom bonded to a carbon atom at the 5-position of the pyrimidine base may be substituted with a group other than a hydrogen atom, in the formulae, each X independently represents S−Z+ or BH3—Z+, Z+ represents a counter cation, each R2 and R3 independently represents a hydrogen atom, or an alkyl group having from 1 to 10 carbon atoms, and R2 and R3 may be bonded to each other to form a ring.