公开(公告)号：US20200242735A1

公开(公告)日：2020-07-30

申请号：US16357850

申请日：2019-03-19

Applicant: TSINGHUA UNIVERSITY

Inventor： Qionghai Dai ,  Zhi Lu ,  Jiamin Wu

IPC: G06T3/40 ,  G02B21/00 ,  H01L27/146 ,  G02B26/10 ,  G02B26/12

Abstract: A super-resolution lattice light field microscopic imaging system includes: a microscope configured to magnify a sample and image the sample onto a first image plane of the microscope; a first relay lens configured to match a numerical aperture of an objective with that of a microlens array; a 2D scanning galvo configured to rotate an angle of a light path in the frequency domain plane; an illuminating system configured to provide uniform illumination on the microlens array to generate SIM pattern illumination; the microlens array, configured to modulate a light beam with a preset angle to a target spatial position at a back focal plane of the microlens array to obtain a modulated image; an image sensor configured to record the modulated image; and a reconstruction module configured to reconstruct a 3D structure of the sample based on the modulated image.

公开(公告)号：US20190005701A1

公开(公告)日：2019-01-03

申请号：US15944403

申请日：2018-04-03

Applicant: TSINGHUA UNIVERSITY

Inventor： Qionghai Dai ,  Mingjie Zhang ,  Jiamin Wu

IPC: G06T15/00 ,  G02B21/06 ,  G02B21/36

Abstract: Microscopic imaging system and method with three-dimensional refractive index tomography are provided. The microscopic imaging system includes: an illumination providing module, configured to provide a beam of parallel lights with a modulated intensity; a microscopic sample, arranged at downstream of the illumination providing module, and configured to modulate a phase of the beam of parallel lights, such that emergent lights passing through the microscopic sample carry information of a three-dimensional refractive index field of the microscopic sample; a microscopic imaging module, arranged at downstream of the microscopic sample, and configured to form an image by using the emergent lights; and a controlling module, configured to process the image to reconstruct three-dimensional refractive index information of the microscopic sample.

公开(公告)号：US11719921B2

公开(公告)日：2023-08-08

申请号：US16869185

申请日：2020-05-07

Applicant: TSINGHUA UNIVERSITY

Inventor： Qionghai Dai ,  You Zhou ,  Jiamin Wu ,  Guoxun Zhang

IPC: G02B21/00 ,  G02B26/08 ,  G02B26/10

CPC classification number: G02B21/0032 ,  G02B21/008 ,  G02B21/0036 ,  G02B21/0076 ,  G02B26/0875 ,  G02B26/101

Abstract: The present disclosure provides a rapid three-dimensional imaging system based on a multi-angle 4Pi microscope. The system includes: an illumination module, configured to obtain a parallel light of which a size covering a projection surface of a spatial light modulator; a wavefront modulation module, configured to place the LCOS device on a Fourier plane of an illumination end; a two-dimensional scanning module, configured to control a light beam to realize a two-dimensional scanning on an object plane; an illumination interference module, configured to generate point spread function PSFs of a 4Pi through an illumination interference to irradiate a fluorescent sample; an imaging module, configured to acquire interference images of two fluorescent signals; and a controller, configured to control the wavefront modulation module to adjust a polarization direction of the light to generate PSFs of the 4Pi with different inclination angles.

公开(公告)号：US11600060B2

公开(公告)日：2023-03-07

申请号：US16893071

申请日：2020-06-04

Applicant: Tsinghua University

Inventor： Qionghai Dai ,  Tao Yan ,  Jiamin Wu ,  Xing Lin

IPC: G06V10/82 ,  G06N3/04 ,  G06N3/067 ,  G06N3/08 ,  G06T3/40

Abstract: The present disclosure discloses a nonlinear all-optical deep-learning system and method with multistage space-frequency domain modulation. The system includes an optical input module, configured to convert input information to optical information, a multistage space-frequency domain modulation module, configured to perform multistage space-frequency domain modulation on the optical information generated by the optical input module so as to generate modulated optical information, and an information acquisition module, configured to transform the modulated optical information onto a Fourier plane or an image plane, and to acquire the transformed optical information so as to generate processed optical information.

公开(公告)号：US11262568B2

公开(公告)日：2022-03-01

申请号：US16357838

申请日：2019-03-19

Applicant: TSINGHUA UNIVERSITY

Inventor： Qionghai Dai ,  You Zhou ,  Jiamin Wu ,  Guoxun Zhang

IPC: G02B21/00 ,  G02B21/08 ,  G02B21/36 ,  G02B5/30

Abstract: A microscopic imaging system and a microscopic imaging method. The system includes: an illumination module configured to generate a laser illumination, an LCOS device located in a Fourier plane of the laser illumination and configured to modulate a phase of the laser illumination, a 4-F system configured to adjust a size of a light beam of the laser illumination, an excitation lens group configured to generate a point illumination focused in a sample plane, a detecting lens group configured to capture an image of a PSF of the point illumination, a camera sensor, and a controller configured to synchronously control a change in a phase pattern of the LCOS device and an image capture of the camera sensor.

公开(公告)号：US10356390B2

公开(公告)日：2019-07-16

申请号：US15459623

申请日：2017-03-15

Applicant: Tsinghua University

Inventor： Qionghai Dai ,  Jijun He ,  Jiamin Wu

IPC: G02B21/06 ,  H04N13/204 ,  G02B27/10 ,  G02B21/36 ,  G02B21/16 ,  G02B27/00 ,  G02B21/00

Abstract: The three-dimensional microscopic imaging system includes: a microscope; a field diaphragm; a narrow-band filter, configured to perform narrow-band filtering; a beam-splitting grating, configured to duplicate a beam into beams with different angles; a lens array with different focal length, configured to perform different phase modulation to each beam with different angles; a micro lens array, configured to modulate the beams with different angles that pass through the lens array with different focal length respectively to different spatial locations at a back focal plane of the micro lens array respectively; and an image sensor configured to record an image corresponding to the modulated beams at the back focal plane of the micro lens array. The system has a simple structure, a cheap cost, and a relatively weak exciting light, and is suit for living samples, and has the adjustable intervals of imaging depths.

公开(公告)号：US11131841B2

公开(公告)日：2021-09-28

申请号：US16357870

申请日：2019-03-19

Applicant: TSINGHUA UNIVERSITY

Inventor： Qionghai Dai ,  Zhi Lu ,  Jiamin Wu

IPC: G02B21/22 ,  G02B21/36 ,  H04N13/106 ,  H04N13/207

Abstract: A scanning light field microscopic imaging system includes: a microscope configured to magnify a sample and image the sample onto a first image plane of the microscope; a relay lens configured to magnify or minify the first image plane; a 2D scanning galvo configured to rotate an angle of a light path in the frequency domain plane; the microlens array configured to modulate a beam with a preset angle to a target spatial position at a back focal plane of the microlens array and modulate the first image plane to obtain a modulated image; an image sensor configured to record the modulated image; and a reconstruction module configured to reconstruct a 3D structure of the sample based on the modulated image acquired from the image sensor.

公开(公告)号：US20200241274A1

公开(公告)日：2020-07-30

申请号：US16357870

申请日：2019-03-19

Applicant: TSINGHUA UNIVERSITY

Inventor： Qionghai Dai ,  Zhi Lu ,  Jiamin Wu

IPC: G02B21/36 ,  H04N13/106 ,  H04N13/207

Abstract: A scanning light field microscopic imaging system includes: a microscope configured to magnify a sample and image the sample onto a first image plane of the microscope; a relay lens configured to magnify or minify the first image plane; a 2D scanning galvo configured to rotate an angle of a light path in the frequency domain plane; the microlens array configured to modulate a beam with a preset angle to a target spatial position at a back focal plane of the microlens array and modulate the first image plane to obtain a modulated image; an image sensor configured to record the modulated image; and a reconstruction module configured to reconstruct a 3D structure of the sample based on the modulated image acquired from the image sensor.

公开(公告)号：US10185137B2

公开(公告)日：2019-01-22

申请号：US15490313

申请日：2017-04-18

Applicant: Tsinghua University

Inventor： Qionghai Dai ,  You Zhou ,  Jiamin Wu ,  Jingtao Fan

IPC: H04N9/47 ,  G02B21/36 ,  H04N5/225 ,  H04N5/235 ,  H04N9/04 ,  H04N5/232 ,  H04N9/64 ,  G02B21/06 ,  G02B13/00

Abstract: The present disclosure relates to Fourier ptychographic microscopy systems and methods. The system includes: a three-color LED array; a microscope, configured to obtain image information with multi-wavelength, and magnify to generate magnified image information; an RGB camera configured to acquire a first color image with a first resolution based on the magnified image information; and a controller, configured to synchronously control the three-color LED array and the RGB camera, in which the three-color LED array is further configured to display a plurality of illumination patterns, the RGB camera is further configured to acquire synchronously a plurality of first color images, and the controller is further configured to restore a single second image with a second resolution according to the plurality of first color images, and the first resolution is less than the second resolution. The present disclosure improves sampling speed.

公开(公告)号：US11169367B2

公开(公告)日：2021-11-09

申请号：US16357811

申请日：2019-03-19

Applicant: TSINGHUA UNIVERSITY

Inventor： Qionghai Dai ,  Zhi Lu ,  Jiamin Wu

IPC: H04N13/204 ,  G02B21/36 ,  G02B21/22

Abstract: Provided are a 3D microscopic imaging method and a 3D microscopic imaging system. The method includes: acquiring a first PSF of a 3D sample from an object plane to a plane of a main camera sensor and a second PSF of the 3D sample from the object plane to a plane of a secondary camera sensor, and generating a first forward projection matrix corresponding to the first PSF and a second forward projection matrix corresponding to the second PSF; acquiring a light field image captured by the main camera sensor and a high resolution image captured by the secondary camera sensor; generating a reconstruction result of the 3D sample by reconstructing the light field image, the first forward projection matrix, the high resolution image and the second forward projection matrix according to a preset algorithm.