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公开(公告)号：US08394641B2
公开(公告)日：2013-03-12
申请号：US13305820
申请日：2011-11-29
Applicant: Theodore Winger
Inventor： Theodore Winger
CPC classification number: C12Q1/40 , B01L3/502784 , B01L2300/0654 , B01L2300/0816 , B01L2300/18 , B01L2400/0427 , B01L2400/043 , C07D311/16 , C07D311/18 , C07D311/20 , C07D405/12 , C07D413/12 , C07H15/203 , C07H17/075 , C12Q1/25 , C12Q1/34 , C12Q1/44 , C12Q1/54 , C12Y302/01023 , G01N21/6452 , G01N33/5438 , G01N2333/916 , G01N2333/926 , G01N2333/938 , G01N2800/04
Abstract: The invention is directed to droplet actuator devices and assay methods. The method may include immobilization of the enzymatic substrate including forming an inclusion complex with the substrate within an aqueous environment in contact with an oil.
Abstract translation: 本发明涉及液滴致动器装置和测定方法。 该方法可以包括固定酶底物，包括在与油接触的水性环境中与基质形成包合物。
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公开(公告)号：US20140246319A1
公开(公告)日：2014-09-04
申请号：US14131846
申请日：2012-07-11
Applicant: Theodore Winger
Inventor： Theodore Winger
IPC: G01N27/447
CPC classification number: G01N27/447 , B01L3/502792 , B01L2300/0816 , B01L2300/12 , B01L2400/0424 , B01L2400/0427 , G01N2800/044
Abstract: Droplet actuators and techniques for droplet-based assays are provided. A method of conducting an assay comprises: incubating a droplet in oil, the droplet comprising an umbelliferone substrate, a sample potentially comprising an enzyme which cleaves the umbelliferone substrate, and a zwitterionic surfactant; and detecting a signal emitted from the droplet.
Abstract translation: 提供了液滴致动器和用于基于液滴的测定技术。 进行测定的方法包括：将液滴在油中孵育，所述液滴包含伞形酮底物，潜在地包含切割伞形酮底物的酶的样品和两性离子表面活性剂; 并检测从液滴发出的信号。
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公开(公告)号：US20130288254A1
公开(公告)日：2013-10-31
申请号：US13390121
申请日：2010-08-13
Applicant: Michael G. Pollack , Vijay Srinivasan , Allen E. Eckhardt , Prasanna Thwar , Zhishan Hua , Arjun Sudarsan , Theodore Winger
Inventor： Michael G. Pollack , Vijay Srinivasan , Allen E. Eckhardt , Prasanna Thwar , Zhishan Hua , Arjun Sudarsan , Theodore Winger
IPC: C12Q1/68
CPC classification number: G01N27/447 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L2200/0668 , B01L2300/0819 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , B01L2400/043 , C12Q1/686 , G01N33/54326 , G01N2035/00425 , G01N2035/1058 , G01N2035/1062 , C12Q2549/101 , C12Q2527/15 , C12Q2527/107 , C12Q2561/113
Abstract: The invention is directed to certain droplet actuated molecular techniques. In one embodiment, the invention provides droplet actuator methods for detection of single nucleotide polymorphisms (SNPs) in a DNA sequence using digital microfluidics, including droplet actuator-based sample preparation and SNP analysis. In another embodiment, the invention provides droplet actuator devices and methods for providing integrated sample preparation and multiplexed detection of an infectious agent, such as HIV. In yet another embodiment, the invention provides droplet actuator devices and techniques for PCR amplification and detection of specific nucleic acid sequences using digital microfluidics, including droplet actuator-based sample preparation and target nucleic acid analysis. In yet another embodiment the invention provides methods for performing hot-start PCR on a droplet actuator. In yet another embodiment, the method of the invention combines PCR amplification with pyrosequencing to investigate specific sequences.
Abstract translation: 本发明涉及某些液滴致动分子技术。 在一个实施方案中，本发明提供用于使用数字微流体（包括基于液滴致动器的样品制备和SNP分析）检测DNA序列中的单核苷酸多态性（SNP）的液滴致动器方法。 在另一个实施方案中，本发明提供了液滴致动器装置和用于提供综合样品制备和多重检测感染因子如HIV的方法。 在另一个实施方案中，本发明提供了使用数字微流体（包括基于液滴致动器的样品制备和靶核酸分析）的PCR扩增和检测特异性核酸序列的液滴致动器装置和技术。 在另一个实施方案中，本发明提供了在液滴致动器上进行热启动PCR的方法。 在另一个实施方案中，本发明的方法将PCR扩增与焦磷酸测序结合以研究特异性序列。
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4.发明申请公开(公告)号：US20080063794A1
公开(公告)日：2008-03-13
申请号：US11855374
申请日：2007-09-14
Applicant: Jainamma Krotz , Daniel Smolko , Howard Reese , Thomas Onofrey , Daguang Wang , Theodore Winger , John Havens
Inventor： Jainamma Krotz , Daniel Smolko , Howard Reese , Thomas Onofrey , Daguang Wang , Theodore Winger , John Havens
CPC classification number: C12Q1/002 , B01D67/003 , B01D69/125 , B01D2323/18 , B01D2323/34 , B01D2323/42 , C09D123/26 , C12Q1/003 , G01N33/5438 , G01N33/545
Abstract: The present invention provides improved synthetic polymer hydrogel permeation layers for use on active electronic matrix devices for biological assays. The present invention includes methods for forming a permeation layer on an array of microelectrodes including the steps of attaching a linker to the surface of the array and providing a polymerization solution that includes a porogen. The surface of the array is then contacted with the polymerization solution and the polymerization solution is then polymerized on the surface of the array to form a permeation layer that is attached o the surface of the array through the linker. The porogen is then removed from the permeation layer, thereby creating void spaces in the permeation layer.
Abstract translation: 本发明提供了用于生物测定的活性电子基质装置上的改进的合成聚合物水凝胶渗透层。 本发明包括在微电极阵列上形成渗透层的方法，包括将接头连接到阵列的表面并提供包含致孔剂的聚合溶液的步骤。 然后将阵列的表面与聚合溶液接触，然后聚合溶液在阵列的表面上聚合，形成通过接头连接在阵列表面上的渗透层。 然后从渗透层除去致孔剂，从而在渗透层中产生空隙。
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公开(公告)号：US09513253B2
公开(公告)日：2016-12-06
申请号：US14131846
申请日：2012-07-11
Applicant: Theodore Winger
Inventor： Theodore Winger
IPC: G01N27/447 , B01L3/00
CPC classification number: G01N27/447 , B01L3/502792 , B01L2300/0816 , B01L2300/12 , B01L2400/0424 , B01L2400/0427 , G01N2800/044
Abstract: Droplet actuators and techniques for droplet-based assays are provided. A method of conducting an assay comprises: incubating a droplet in oil, the droplet comprising an umbelliferone substrate, a sample potentially comprising an enzyme which cleaves the umbelliferone substrate, and a zwitterionic surfactant; and detecting a signal emitted from the droplet.
Abstract translation: 提供了液滴执行器和基于液滴的测定技术。 进行测定的方法包括：在油中孵育液滴，所述液滴包含伞形酮底物，潜在地包含切割伞形酮底物的酶的样品和两性离子表面活性剂; 并检测从液滴发出的信号。
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Patent Agency Ranking
公开(公告)号：US20120136147A1
公开(公告)日：2012-05-31
申请号：US13305820
申请日：2011-11-29
Applicant: Theodore Winger
Inventor： Theodore Winger
IPC: C08B37/16
CPC classification number: C12Q1/40 , B01L3/502784 , B01L2300/0654 , B01L2300/0816 , B01L2300/18 , B01L2400/0427 , B01L2400/043 , C07D311/16 , C07D311/18 , C07D311/20 , C07D405/12 , C07D413/12 , C07H15/203 , C07H17/075 , C12Q1/25 , C12Q1/34 , C12Q1/44 , C12Q1/54 , C12Y302/01023 , G01N21/6452 , G01N33/5438 , G01N2333/916 , G01N2333/926 , G01N2333/938 , G01N2800/04
Abstract: The invention is directed to droplet actuator devices and assay methods. The method may include immobilization of the enzymatic substrate including forming an inclusion complex with the substrate within an aqueous environment in contact with an oil.
Abstract translation: 本发明涉及液滴致动器装置和测定方法。 该方法可以包括固定酶底物，包括在与油接触的水性环境中与基质形成包合物。
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7.发明申请Enzymatic Assays Using Umbelliferone Substrates with Cyclodextrins in Droplets of Oil 有权使用伞形酮底物与环糊精在油滴中的酶检测公开(公告)号：US20110118132A1
公开(公告)日：2011-05-19
申请号：US13012831
申请日：2011-01-25
Applicant: Theodore Winger , Allen E. Eckhardt , Carrie Graham , Ramakrishna Sista , Vamsee K. Pamula , Tong Wang , Yalin Xiong , Ning Wu , Sanjay Saha , Gajendrasinh Balvantsinh Raolji , Raveendra Dayam
Inventor： Theodore Winger , Allen E. Eckhardt , Carrie Graham , Ramakrishna Sista , Vamsee K. Pamula , Tong Wang , Yalin Xiong , Ning Wu , Sanjay Saha , Gajendrasinh Balvantsinh Raolji , Raveendra Dayam
CPC classification number: C12Q1/00
Abstract: The invention is directed to droplet actuator devices and assay methods. The method may include, among other things, a method of conducting enzymatic assays using umbelliferone substrates with cyclodextrins in droplets in oil, the method including incubating a droplet in oil, the droplet preferably comprising an umbelliferone substrate, a sample, and a cyclodextrin compound. The methods may further include a method of substantially eliminating cross-contamination between droplets in enzymatic containing substrate-based bioassays. The method may include immobilization of the enzymatic substrate including forming an inclusion complex with the substrate for stabilizing the substrate within an aqueous environment, wherein the inclusion complex may be formed using cyclodextrins. In yet another embodiment, the invention may provide a method of enhancing hydrolysis of enzymatic substrates. The methods may further include a method of forming an inclusion complex with the substrate for stabilizing the substrate within an aqueous environment, wherein the substrate may be a 4-MU- or HMU containing substrate. The inclusion complex may be formed using cyclodextrin.
Abstract translation: 本发明涉及液滴致动器装置和测定方法。 该方法尤其可以包括使用具有环糊精的伞形酮底物在油中的液滴进行酶测定的方法，所述方法包括将液滴在油中孵育，所述液滴优选包含伞形酮底物，样品和环糊精化合物。 所述方法还可以包括基本上消除含酶底物生物测定中的液滴之间的交叉污染的方法。 该方法可以包括固定酶底物，包括与底物形成包合物以使其在水性环境中稳定，其中包合物可以使用环糊精形成。 在另一个实施方案中，本发明可提供增强酶底物水解的方法。 所述方法还可以包括在水性环境中与基材形成包含配合物以稳定基材的方法，其中所述基材可以是含有4-MU-或HMU的底物。 包合物可以使用环糊精形成。
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公开(公告)号：US20050158451A1
公开(公告)日：2005-07-21
申请号：US11022200
申请日：2004-12-23
Applicant: Havens John , Theodore Winger , Jain Krotz , Smolko Dan , Thomas Onofrey
Inventor： Havens John , Theodore Winger , Jain Krotz , Smolko Dan , Thomas Onofrey
CPC classification number: G01N27/40 , B01J2219/00653 , B01J2219/00659 , B01J2219/00722 , B01J2219/00725 , C40B40/06 , C40B40/10 , G01N33/5438
Abstract: Electronically addressable microchips having covalently bound permeation layers and methods of making such covalently bonded permeation layers to microchips are provided. The covalent bonding is derived from combining the use of electrodes with silane derivatives. Such chemistry provides the ability to apply an electronic bias to the electrodes of the microchip while preventing permeation layer delaminating from the electrode surface. Methods for covalently attaching the permeation layer to the microchips are also described.
Abstract translation: 提供具有共价结合的渗透层的电子寻址微芯片以及将这种共价键合的渗透层制成微芯片的方法。 共价键是从使用电极与硅烷衍生物组合得到的。 这种化学提供了将电子偏压施加到微芯片的电极的能力，同时防止从电极表面分层的渗透层。 还描述了将渗透层共价连接到微芯片的方法。
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公开(公告)号：US08926065B2
公开(公告)日：2015-01-06
申请号：US13238872
申请日：2011-09-21
Applicant: Theodore Winger
Inventor： Theodore Winger
CPC classification number: B05B5/087 , B41J2/14 , B41J2/1606 , B41J2002/14322 , B41J2002/14395 , Y10T428/31504 , Y10T428/3154 , Y10T428/31855
Abstract: A microfluidic device having a substrate with an electrically conductive element made using a conductive ink layer underlying a hydrophobic layer.
Abstract translation: 一种微流体装置，其具有使用在疏水层下面的导电油墨层制成的导电元件的基板。
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公开(公告)号：US20120044299A1
公开(公告)日：2012-02-23
申请号：US13238872
申请日：2011-09-21
Applicant: Theodore Winger
Inventor： Theodore Winger
CPC classification number: B05B5/087 , B41J2/14 , B41J2/1606 , B41J2002/14322 , B41J2002/14395 , Y10T428/31504 , Y10T428/3154 , Y10T428/31855
Abstract: A microfluidic device having a substrate with an electrically conductive element made using a conductive ink layer underlying a hydrophobic layer.
Abstract translation: 一种微流体装置，其具有使用在疏水层下面的导电油墨层制成的导电元件的基板。
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