公开(公告)号：US10273476B2

公开(公告)日：2019-04-30

申请号：US15583564

申请日：2017-05-01

Applicant: UNIVERSITY OF IOWA RESEARCH FOUNDATION

Inventor： Liu Hong ,  Brad Amendt ,  Thad Sharp ,  Aliasger Salem

IPC: A61K48/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/113 ,  A61L27/24 ,  A61L27/56 ,  A61L27/54 ,  A61K31/713 ,  A61K9/16 ,  C12N7/00 ,  A61K9/00 ,  A61K9/51

Abstract: In certain embodiments, the present invention provides the use of microRNA (miR)-200a and/or miR-200c to inhibit ossification and bone formation. In certain embodiments, the present invention provides the use of miR-200a inhibitor to stimulate bone regeneration.

公开(公告)号：US20170321190A1

公开(公告)日：2017-11-09

申请号：US15526282

申请日：2015-08-13

Applicant: UNIVERSITY OF IOWA RESEARCH FOUNDATION

Inventor： Brad A. Amendt ,  Thad Sharp

IPC: C12N5/077 ,  A61K35/28

CPC classification number: C12N5/0652 ,  A61K35/28 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/65 ,  C12N2501/998 ,  C12N2506/13

Abstract: The invention provides in certain embodiments, a method of generating a re-programmed differentiated epithelial cell comprising (a) contacting a non-stem somatic cell obtained from a subject with an effective amount of a de-differentiation agent to form a de-differentiated cell, and (b) transfecting the de-differentiated cell with an expression cassette comprising a promoter operably linked to a nucleic acid encoding a conversion agent to form a re-programmed differentiated cell. The invention also provides in certain embodiments, a method of generating a re-programmed differentiated epithelial cell comprising (a) contacting a non-stem somatic cell obtained from a subject with an effective amount of a de-differentiation agent to form a de-differentiated cell, and (b) contacting the de-differentiated cell with a conversion agent to form a re-programmed differentiated cell. The invention provides in certain embodiments, re-programmed differentiated epithelial cells, and methods of using these re-programmed differentiated epithelial cells to repair or re-generate tissue in vivo.

公开(公告)号：US20170314020A1

公开(公告)日：2017-11-02

申请号：US15583564

申请日：2017-05-01

Applicant: UNIVERSITY OF IOWA RESEARCH FOUNDATION

Inventor： Liu Hong ,  Brad Amendt ,  Thad Sharp ,  Aliasger Salem

IPC: C12N15/113 ,  A61K31/713 ,  C12N7/00 ,  A61L27/54 ,  A61L27/56 ,  A61K9/16 ,  A61L27/24 ,  A61K48/00

CPC classification number: C12N15/113 ,  A61K9/0019 ,  A61K9/1641 ,  A61K9/1647 ,  A61K9/5153 ,  A61K31/713 ,  A61K48/00 ,  A61L27/24 ,  A61L27/54 ,  A61L27/56 ,  A61L2300/258 ,  A61L2300/412 ,  A61L2300/624 ,  A61L2430/02 ,  C12N7/00 ,  C12N2310/141 ,  C12N2320/31 ,  C12N2320/32 ,  C12N2330/51 ,  C12N2740/15043

Abstract: In certain embodiments, the present invention provides the use of microRNA (miR)-200a and/or miR-200c to inhibit ossification and bone formation. In certain embodiments, the present invention provides the use of miR-200a inhibitor to stimulate bone regeneration.

公开(公告)号：US10920195B2

公开(公告)日：2021-02-16

申请号：US15526282

申请日：2015-08-13

Applicant: UNIVERSITY OF IOWA RESEARCH FOUNDATION

Inventor： Brad A. Amendt ,  Thad Sharp

IPC: C12N5/077 ,  A61K35/28

Abstract: The invention provides in certain embodiments, a method of generating a re-programmed differentiated epithelial cell comprising (a) contacting a non-stem somatic cell obtained from a subject with an effective amount of a de-differentiation agent to form a de-differentiated cell, and (b) transfecting the de-differentiated cell with an expression cassette comprising a promoter operably linked to a nucleic acid encoding a conversion agent to form a re-programmed differentiated cell. The invention also provides in certain embodiments, a method of generating a re-programmed differentiated epithelial cell comprising (a) contacting a non-stem somatic cell obtained from a subject with an effective amount of a de-differentiation agent to form a de-differentiated cell, and (b) contacting the de-differentiated cell with a conversion agent to form a re-programmed differentiated cell. The invention provides in certain embodiments, re-programmed differentiated epithelial cells, and methods of using these re-programmed differentiated epithelial cells to repair or re-generate tissue in vivo.

公开(公告)号：US10717980B2

公开(公告)日：2020-07-21

申请号：US16299692

申请日：2019-03-12

Applicant: UNIVERSITY OF IOWA RESEARCH FOUNDATION

Inventor： Liu Hong ,  Brad Amendt ,  Thad Sharp ,  Aliasger Salem

IPC: C07H21/02 ,  C07H21/04 ,  C12N15/113 ,  A61L27/24 ,  A61L27/56 ,  A61L27/54 ,  A61K31/713 ,  A61K9/16 ,  C12N7/00 ,  A61K9/00 ,  A61K9/51 ,  A61K48/00

Abstract: In certain embodiments, the present invention provides the use of microRNA (miR)-200a to inhibit ossification and bone formation.

公开(公告)号：US10669543B2

公开(公告)日：2020-06-02

申请号：US15543816

申请日：2016-01-15

Applicant: University of Iowa Research Foundation

Inventor： Liu Hong ,  Brad A. Amendt ,  Aliasger K. Salem ,  Satheesh Elangovan ,  Gustavo Avila Ortiz ,  Thad Sharp

IPC: C12N15/113 ,  A61P19/04 ,  A61P19/02 ,  A61K47/34 ,  A61K47/14 ,  A61K47/18

Abstract: Compositions and methods to prevent, inhibit or treat aveolar or periodontal bone loss, enhance bone regeneration, prevent, inhibit or treat peri-implantitis or periodontitis, e.g., periodontitis or peri-implantitis associated with bone loss, or to prevent, inhibit or treat osteoarthritis are provided.

公开(公告)号：US20190264206A1

公开(公告)日：2019-08-29

申请号：US16299692

申请日：2019-03-12

Applicant: UNIVERSITY OF IOWA RESEARCH FOUNDATION

Inventor： Liu Hong ,  Brad Amendt ,  Thad Sharp ,  Aliasger Salem

IPC: C12N15/113 ,  A61K9/00 ,  A61K9/51 ,  A61K31/713 ,  A61L27/24 ,  A61L27/54 ,  A61K9/16 ,  C12N7/00 ,  A61L27/56

Abstract: In certain embodiments, the present invention provides the use of microRNA (miR)-200a to inhibit ossification and bone formation.