公开(公告)号：US12180581B2

公开(公告)日：2024-12-31

申请号：US17166460

申请日：2021-02-03

Applicant: Waters Technologies Corporation

Inventor： Stephen J. Shiner ,  Cheryl Boissel ,  Matthew A. Lauber ,  Kevin Wyndham

IPC: C23C16/02 ,  C09D143/04 ,  C23C14/04 ,  C23C14/12 ,  C23C14/16 ,  C23C14/58 ,  G01N30/56 ,  G01N30/60

Abstract: A chromatographic device including a coated metallic frit is disclosed. The coating is provided over the fluid exposed surfaces of the frit, thereby covering metallic surfaces to prevent interaction with an analyte in the flowstream. This technology relates to the use of vapor deposition coated frit(s) in a liquid flow path for improved chromatography. More specifically, this technology relates to liquid chromatographic devices for separating analytes in a sample having coated frit(s) within an uncoated metallic fluidic flow path (i.e., the column tube or channel is formed of stainless steel, titanium (pure or alloyed), or some mixture of stainless steel and titanium) and does not include the coating applied to the frit.

公开(公告)号：US12138617B2

公开(公告)日：2024-11-12

申请号：US17477347

申请日：2021-09-16

Applicant: Waters Technologies Corporation

Inventor： Nicole L. Lawrence ,  Yeliz Tunc Sarisozen ,  Darryl W. Brousmiche ,  Matthew A. Lauber

IPC: B01J20/289 ,  B01J20/10 ,  B01J20/26 ,  B01J20/28

Abstract: The present disclosure is directed to stationary phase materials for performing size exclusion chromatography. Embodiments of the present disclosure feature hydroxy-terminated polyethylene glycol surface modified silica particle stationary phase materials, which are optionally also methoxy-terminated polyethylene glycol surface modified.

公开(公告)号：US12121829B2

公开(公告)日：2024-10-22

申请号：US16800928

申请日：2020-02-25

Applicant: Waters Technologies Corporation

Inventor： Mathew H. DeLano ,  Matthew A. Lauber ,  Michael Donegan ,  Robert A. Jencks ,  Stephen J. Shiner

IPC: B01D15/22 ,  C08J7/06 ,  G01N30/02 ,  G01N30/32 ,  G01N30/60

CPC classification number: B01D15/22 ,  C08J7/065 ,  G01N30/32 ,  G01N30/6026 ,  G01N30/603 ,  G01N30/6052 ,  G01N2030/027 ,  G01N2030/324

Abstract: The present disclosure relates to a filter. The filter includes a porous element, a compression element and a housing. At least a portion of the porous element is coated with an alkylsilyl coating. The compression element is configured to receive the porous element thereby forming an assembly. The housing has an opening formed therein. The opening is configured to receive the assembly. The assembly is retained within the opening when the assembly is received therein.

公开(公告)号：US20240335830A1

公开(公告)日：2024-10-10

申请号：US18749930

申请日：2024-06-21

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Mathew H. DeLano ,  Beatrice Muriithi ,  Anna Boardman

IPC: B01L3/00 ,  B01D15/38 ,  B01D15/42

CPC classification number: B01L3/5023 ,  B01D15/3809 ,  B01D15/424 ,  B01L2200/0631 ,  B01L2200/0668 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2400/0409 ,  B01L2400/0433

Abstract: A sample preparation device is provided for enriching a component of a sample. The sample preparation device includes a surface in fluid communication with the sample, an alkylsilyl coating disposed on the surface, and an affinity ligand or an enzyme covalently bonded to the alkylsilyl coating.

公开(公告)号：US20240092959A1

公开(公告)日：2024-03-21

申请号：US18174309

申请日：2023-02-24

Applicant: Waters Technologies Corporation

Inventor： Nicole L. Lawrence ,  Peng Chen ,  Mingcheng Xu ,  Daniel P. Walsh ,  Matthew A. Lauber

IPC: C08F283/06

CPC classification number: C08F283/06

Abstract: The present disclosure pertains to non-porous composite particles that are non-porous, polymer-based particles. In various embodiments, a non-porous polymer core is surface modified. In various embodiments, a non-porous hybrid organic-inorganic material is in contact with the modified surface of the core, and a bonding material is in contact with the non-porous hybrid organic-inorganic material. The present disclosure pertains to chromatographic separation devices that comprise such non-porous composite particles.

公开(公告)号：US20230364585A1

公开(公告)日：2023-11-16

申请号：US18144514

申请日：2023-05-08

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Catalin Doneanu ,  Weibin Chen ,  Ying Qing Yu ,  Jennifer M. Nguyen ,  Fabrice Gritti ,  Martin Gilar

IPC: B01J20/288 ,  B01J20/32 ,  B01J20/10 ,  B01J20/28

CPC classification number: B01J20/288 ,  B01J20/103 ,  B01J20/28004 ,  B01J20/3204 ,  B01J2220/54

Abstract: The present disclosure discusses a method of separating and/or purifying polynucleotides. The method includes injecting a sample into a chromatographic column that is packed with a porous sorbent having a pore size that substantially excludes the polynucleotides from the sorbent. This restricted access to the sorbent allows separation of large polynucleotides from each other and from smaller molecular weight impurities.

公开(公告)号：US20220412963A1

公开(公告)日：2022-12-29

申请号：US17338022

申请日：2021-06-03

Applicant: Waters Technologies Corporation

Inventor： Darryl W. Brousmiche ,  Matthew A. Lauber

IPC: G01N33/533 ,  C07D215/48

Abstract: Novel reagents comprising MS active, fluorescent compounds having an activated functionality for reaction with aldehydes and useful in labeling biomolecules such as glycans and methods of making the same are taught and described.

公开(公告)号：US20220082532A1

公开(公告)日：2022-03-17

申请号：US17471449

申请日：2021-09-10

Applicant: Waters Technologies Corporation

Inventor： Amit Patel ,  Moon Chul Jung ,  Susan Rzewuski ,  Thomas H. Walter ,  Matthew A. Lauber ,  Kevin Wyndham ,  Mathew DeLano ,  Jennifer Simeone

IPC: G01N30/16 ,  G01N30/86 ,  G01N30/72 ,  G01N30/74

Abstract: The present disclosure is directed to methods for evaluating system inertness, such as the inertness of a LC or other fluidic system. Some methods are directed to tests wherein the column has been removed prior to injecting a sample including a positive (e.g., metal reacting moiety) control into the system. Some methods can include: (1) repeatedly injecting the sample into a system, the system comprising: fluidic paths wherein interior surfaces of the fluidic paths define wetted surfaces, and wherein at least a portion of the wetted surfaces of the fluidic flow path are coated with an inert coating, wherein the inert coating is inert to at least one analyte in the sample; (2) detecting a value associated with the positive control; and (3) analyzing values associated with the detected positive control to determine system inertness.

公开(公告)号：US20210388022A1

公开(公告)日：2021-12-16

申请号：US17314748

申请日：2021-05-07

Applicant: Waters Technologies Corporation

Inventor： Xiaoxiao Liu ,  Wenjing Li ,  Matthew A. Lauber

IPC: C07K1/113 ,  C07C279/04 ,  C12Q1/37 ,  C07D487/04

Abstract: The present disclosure relates to a system for a composition for protein denaturation. The composition includes a non-nucleophilic denaturant comprising a substituted guanidine, wherein the denaturant has a pKa value greater than about 10, and wherein the concentration of the substituted guanidine is less than 250 mM.

公开(公告)号：US20210349061A1

公开(公告)日：2021-11-11

申请号：US17308637

申请日：2021-05-05

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Kevin Wyndham ,  Bonnie Alden ,  Dominic Foley ,  David Morrissey

IPC: G01N30/56 ,  G01N30/88 ,  G01N30/72

Abstract: The present disclosure relates to the use of vapor deposition coated flow paths for improved chromatography and sample analysis using liquid chromatography-mass spectrometry (LC/MS) or liquid chromatography-optical detection (LC/UV). More specifically, this technology relates to separating and quantitation of analytes (e.g., phospho prodrugs and its phosphorylated metabolites) from a sample matrix (e.g., mammalian blood, plasma) using chromatographic devices and fluidic systems having coated flow paths. The LC-MS or LC-UV techniques provide improved recovery, peak shape and dynamic range in the analysis of the prodrug and its metabolites.