公开(公告)号：US08232052B2

公开(公告)日：2012-07-31

申请号：US12868598

申请日：2010-08-25

Applicant: Yuichi Ono ,  Yasuko Nakagawa ,  Yoshimasa Sakamoto

Inventor： Yuichi Ono ,  Yasuko Nakagawa ,  Yoshimasa Sakamoto

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q2600/158

Abstract: In neuron transplantation therapy, in terms of safety, it is preferable to use a cell population consisting only of a desired type of cells, and to use postmitotic neurons in consideration to avoid the risk of tumorigenesis. Moreover, greater therapeutic effects would be expected through the use of earlier progenitor cells in consideration of post-transplantation viability, proper network formation ability, and such.According to the present invention, Lrp4, a gene that is specifically expressed in dopaminergic neuron proliferative progenitor cells prior to cell cycle exit, was identified. The use of Lrp4 expression in cells as an index allows for the isolation. of cells suitable for transplantation therapy of neurodegenerative diseases such as Parkinson's disease in terms of safety, survival rate, and network formation ability.

Abstract translation: 在神经元移植治疗中，在安全性方面，优选使用仅由期望类型的细胞组成的细胞群，并考虑使用神经元神经元以避免肿瘤发生的风险。 此外，考虑到移植后的生存力，适当的网络形成能力等，通过使用较早的祖细胞可以预期更大的治疗效果。 根据本发明，鉴定了在细胞周期退出之前在多巴胺能神经元增殖祖细胞中特异性表达的基因Lrp4。 在细胞中使用Lrp4表达作为指标，可以进行分离。 的细胞在安全性，存活率和网络形成能力方面适用于帕金森病等神经变性疾病的移植治疗。

公开(公告)号：US20110229889A1

公开(公告)日：2011-09-22

申请号：US13071450

申请日：2011-03-24

Applicant: Yuichi Ono ,  Yasuko Nakagawa ,  Tomoya Nakatani ,  Yoshimasa Sakamoto

Inventor： Yuichi Ono ,  Yasuko Nakagawa ,  Tomoya Nakatani ,  Yoshimasa Sakamoto

IPC: C12Q1/68 ,  C07H21/00

CPC classification number: G01N33/56966 ,  C07K16/18 ,  C12Q1/6881 ,  C12Q2600/158

Abstract: The present invention is a probe, a primer, and an antibody, for detecting a dopaminergic neuron proliferative progenitor cell. According to the present invention, there is provided a polynucleotide probe and a polynucleotide primer for use in the detection or selection of a dopaminergic neuron proliferative progenitor cell, which can hybridize with a polynucleotide consisting of a nucleotide sequence of an Msx1 gene or an Msx2 gene, or a complementary sequence thereto, and an antibody against an Msx1 protein or an Msx2 protein, or a part thereof for use in the detection or selection of a dopaminergic neuron proliferative progenitor cell.

Abstract translation: 本发明是用于检测多巴胺能神经元增殖祖细胞的探针，引物和抗体。 根据本发明，提供了用于检测或选择多巴胺能神经元增殖祖细胞的多核苷酸探针和多核苷酸引物，其可以与由Msx1基因或Msx2基因的核苷酸序列组成的多核苷酸杂交 ，或其互补序列，以及针对用于检测或选择多巴胺能神经元增殖祖细胞的Msx1蛋白或Msx2蛋白或其一部分的抗体。

公开(公告)号：US5344260A

公开(公告)日：1994-09-06

申请号：US37378

申请日：1993-03-26

Applicant: Shigeru Suzuki ,  Motonori Kobara ,  Yasuhiro Hisatomi ,  Yoshinori Yamaguchi ,  Hiroshi Yonetani ,  Kiyoyuki Fukushima ,  Yoshimasa Sakamoto ,  Toru Yagami ,  Toshihide Kamei

Inventor： Shigeru Suzuki ,  Motonori Kobara ,  Yasuhiro Hisatomi ,  Yoshinori Yamaguchi ,  Hiroshi Yonetani ,  Kiyoyuki Fukushima ,  Yoshimasa Sakamoto ,  Toru Yagami ,  Toshihide Kamei

IPC: B23B29/034 ,  B23B39/00 ,  B23B41/00

CPC classification number: B23B29/03446 ,  Y10T408/85892 ,  Y10T408/85895 ,  Y10T82/12

Abstract: A tool holder is detachably secured to the lower end of a main spindle by means of a clamping nut having a female screw cooperating with a male screw of the main spindle such that keys are inserted into corresponding key recesses formed in an upper end face of the tool holder. Within the main spindle, there is arranged a driving shaft movably in an axial direction. Within the tool holder, a piston member is arranged movably in the axial direction. To a lower end of the driving shaft is provided a projection having a circular recess formed in an outer surface thereof. To an upper end portion of the piston member are secured a plurality of steel balls movably in a radial direction. A lower end of the piston member is formed as an inclined surface which is engaged with a cam surface of a tool fitting plate arranged movably in the radial direction. When the driving shaft is moved downward, the balls are inserted into the circular recess formed in the projection of the driving shaft so that the driving shaft is coupled with the piston member. Therefore, by moving the driving shaft up and down, the piston member is also moved up and down and this movement of the piston member is converted into the radial movement of the tool fitting plate. By moving the driving shaft upward, the tool holder may be exchanged automatically.

Abstract translation: 工具架借助于具有与主轴的外螺纹配合的内螺纹的夹紧螺母可拆卸地固定在主轴的下端，使得键被插入形成在主轴的上端面中的对应的键槽中 工具托架。 在主轴内，沿轴向可动地设置驱动轴。 在工具架内，活塞部件沿轴向移动。 在驱动轴的下端设置有在其外表面形成有圆形凹部的突起。 活塞构件的上端部沿径向移动地固定多个钢球。 活塞构件的下端形成为与径向可动地配置的工具装配板的凸轮面接合的倾斜面。 当驱动轴向下移动时，将球插入形成在驱动轴的突起中的圆形凹槽中，使得驱动轴与活塞构件联接。 因此，通过上下移动驱动轴，活塞部件也上下移动，活塞部件的移动转换成工具装配板的径向运动。 通过向上移动驱动轴，刀架可以自动更换。

公开(公告)号：US08932592B2

公开(公告)日：2015-01-13

申请号：US13504742

申请日：2010-10-28

Applicant: Miyuki Nishimura ,  Yoshimasa Sakamoto ,  Tetsu Kawano ,  Toshio Imai

Inventor： Miyuki Nishimura ,  Yoshimasa Sakamoto ,  Tetsu Kawano ,  Toshio Imai

IPC: A61K39/395 ,  C07K16/24 ,  C12N15/13 ,  C12N5/10 ,  C12P21/02

CPC classification number: C07K16/24 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/76 ,  Y10S514/894

Abstract: The present invention features compositions and methods related to humanized antibodies and FKN-binding fragments thereof that bind fractalkine.

Abstract translation: 本发明的特征在于与人源化抗体相关的组合物和方法，其结合分子链的FKN结合片段。

公开(公告)号：US08067161B2

公开(公告)日：2011-11-29

申请号：US12064018

申请日：2006-08-18

Applicant: Yuichi Ono ,  Yasuko Nakagawa ,  Tomoya Nakatani ,  Yoshimasa Sakamoto

Inventor： Yuichi Ono ,  Yasuko Nakagawa ,  Tomoya Nakatani ,  Yoshimasa Sakamoto

IPC: C07H21/04 ,  C12Q1/68

CPC classification number: C07K16/18 ,  C07K14/47 ,  C12Q1/6881 ,  C12Q2600/158 ,  G01N33/5073

Abstract: The present invention is a probe, a primer, and an antibody, for detecting a dopaminergic neuron proliferative progenitor cell. According to the present invention, there is provided a polynucleotide probe and a polynucleotide primer for use in the detection or selection of a dopaminergic neuron proliferative progenitor cell, which can hybridize with a polynucleotide consisting of a nucleotide sequence of a Nato3 gene, or a complementary sequence thereto, and an antibody against a Nato3 protein, or a part thereof for use in the detection or selection of a dopaminergic neuron proliferative progenitor cell.

Abstract translation: 本发明是用于检测多巴胺能神经元增殖祖细胞的探针，引物和抗体。 根据本发明，提供了用于检测或选择多巴胺能神经元增殖祖细胞的多核苷酸探针和多核苷酸引物，其可与由Nato3基因的核苷酸序列或互补的多核苷酸组成的多核苷酸杂交 序列，以及针对Nato3蛋白或其部分的抗体用于检测或选择多巴胺能神经元增殖祖细胞。

公开(公告)号：US20100323366A1

公开(公告)日：2010-12-23

申请号：US12868598

申请日：2010-08-25

Applicant: Yuichi Ono ,  Yasuko Nakagawa ,  Yoshimasa Sakamoto

Inventor： Yuichi Ono ,  Yasuko Nakagawa ,  Yoshimasa Sakamoto

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q2600/158

Abstract: In neuron transplantation therapy, in terms of safety, it is preferable to use a cell population consisting only of a desired type of cells, and to use postmitotic neurons in consideration to avoid the risk of tumorigenesis. Moreover, greater therapeutic effects would be expected through the use of earlier progenitor cells in consideration of post-transplantation viability, proper network formation ability, and such.According to the present invention, Lrp4, a gene that is specifically expressed in dopaminergic neuron proliferative progenitor cells prior to cell cycle exit, was identified. The use of Lrp4 expression in cells as an index allows for the isolation. of cells suitable for transplantation therapy of neurodegenerative diseases such as Parkinson's disease in terms of safety, survival rate, and network formation ability.

Abstract translation: 在神经元移植治疗中，在安全性方面，优选使用仅由期望类型的细胞组成的细胞群，并考虑使用神经元神经元以避免肿瘤发生的风险。 此外，考虑到移植后的生存力，适当的网络形成能力等，通过使用较早的祖细胞可以预期更大的治疗效果。 根据本发明，鉴定了在细胞周期退出之前在多巴胺能神经元增殖祖细胞中特异性表达的基因Lrp4。 在细胞中使用Lrp4表达作为指标，可以进行分离。 的细胞在安全性，存活率和网络形成能力方面适用于帕金森病等神经变性疾病的移植治疗。

公开(公告)号：US20100216164A1

公开(公告)日：2010-08-26

申请号：US12392063

申请日：2009-02-24

Applicant: Masakazu Takeuchi ,  Keiko Sato ,  Yoshimasa Sakamoto ,  Maki Tawarada ,  Toshihisa Ohtsuka

Inventor： Masakazu Takeuchi ,  Keiko Sato ,  Yoshimasa Sakamoto ,  Maki Tawarada ,  Toshihisa Ohtsuka

IPC: G01N33/567

CPC classification number: C07K14/47

Abstract: The present invention provides the mPrickle gene which encodes a protein present in mammalian PSD fractions. The mPrickle protein is localized in synapses, and binds to the scaffold protein PSD-95. Precipitation of endogenous mPrickle using an anti-mPrickle antibody results in the coprecipitation of NMDA receptors, and thus, mPrickle can be used in drug delivery systems that target NMDA receptors. NMDA receptors are closely related to learning and memory, and are also suggested to be involved in mental disorders. Thus, in the future, mPrickle is expected to be applicable to the diagnosis and/or treatment of neurodegenerative diseases associated with learning/memory, such as mental deterioration and dementia.

Abstract translation: 本发明提供编码哺乳动物PSD级分中存在的蛋白质的mPrickle基因。 mPrickle蛋白定位在突触中，并结合到支架蛋白PSD-95。 使用抗mPrickle抗体沉淀内源性mPrickle导致NMDA受体的共沉淀，因此，mPrickle可用于靶向NMDA受体的药物递送系统。 NMDA受体与学习记忆密切相关，也被认为与精神障碍有关。 因此，将来，mPrickle预期适用于与学习/记忆相关的神经变性疾病的诊断和/或治疗，例如精神恶化和痴呆。

公开(公告)号：US20100203505A1

公开(公告)日：2010-08-12

申请号：US12064019

申请日：2006-08-18

Applicant: Yuichi Ono ,  Yasuko Nakagawa ,  Tomoya Nakatani ,  Yoshimasa Sakamoto

Inventor： Yuichi Ono ,  Yasuko Nakagawa ,  Tomoya Nakatani ,  Yoshimasa Sakamoto

IPC: C12Q1/68 ,  C07H21/04 ,  C07K16/00

CPC classification number: G01N33/56966 ,  C07K16/18 ,  C12Q1/6881 ,  C12Q2600/158

Abstract: The present invention is a probe, a primer, and an antibody, for detecting a dopaminergic neuron proliferative progenitor cell. According to the present invention, there is provided a polynucleotide probe and a polynucleotide primer for use in the detection or selection of a dopaminergic neuron proliferative progenitor cell, which can hybridize with a polynucleotide consisting of a nucleotide sequence of an Msx1 gene or an Msx2 gene, or a complementary sequence thereto, and an antibody against an Msx1 protein or an Msx2 protein, or a part thereof for use in the detection or selection of a dopaminergic neuron proliferative progenitor cell.

Abstract translation: 本发明是用于检测多巴胺能神经元增殖祖细胞的探针，引物和抗体。 根据本发明，提供了用于检测或选择多巴胺能神经元增殖祖细胞的多核苷酸探针和多核苷酸引物，其可以与由Msx1基因或Msx2基因的核苷酸序列组成的多核苷酸杂交 ，或其互补序列，以及针对用于检测或选择多巴胺能神经元增殖祖细胞的Msx1蛋白或Msx2蛋白或其一部分的抗体。

公开(公告)号：US20060292669A1

公开(公告)日：2006-12-28

申请号：US10536586

申请日：2003-11-28

Applicant: Masakazu Takeuchi ,  Keiko Sato ,  Yoshimasa Sakamoto ,  Maki Tawarada ,  Toshihisa Ohtsuka

Inventor： Masakazu Takeuchi ,  Keiko Sato ,  Yoshimasa Sakamoto ,  Maki Tawarada ,  Toshihisa Ohtsuka

IPC: C07K14/705 ,  C07K16/28 ,  C07H21/04 ,  C12P21/06

CPC classification number: C07K14/47

Abstract: The present invention provides the mPrickle gene which encodes a protein present in mammalian PSD fractions. The mPrickle protein is localized in synapses, and binds to the scaffold protein PSD-95. Precipitation of endogenous mPrickle using an anti-mPrickle antibody results in the coprecipitation of NMDA receptors, and thus, mPrickle can be used in drug delivery systems that target NMDA receptors. NMDA receptors are closely related to learning and memory, and are also suggested to be involved in mental disorders. Thus, in the future, mPrickle is expected to be applicable to the diagnosis and/or treatment of neurodegenerative diseases associated with learning/memory, such as mental deterioration and dementia.

Abstract translation: 本发明提供编码哺乳动物PSD级分中存在的蛋白质的mPrickle基因。 mPrickle蛋白定位在突触中，并结合到支架蛋白PSD-95。 使用抗mPrickle抗体沉淀内源性mPrickle导致NMDA受体的共沉淀，因此，mPrickle可用于靶向NMDA受体的药物递送系统。 NMDA受体与学习记忆密切相关，也被认为与精神障碍有关。 因此，将来，mPrickle预期适用于与学习/记忆相关的神经变性疾病的诊断和/或治疗，例如精神恶化和痴呆。

公开(公告)号：US20060240432A1

公开(公告)日：2006-10-26

申请号：US10543003

申请日：2004-01-23

Applicant: Yuichi Ono ,  Yasuko Nakagawa ,  Yoshimasa Sakamoto

Inventor： Yuichi Ono ,  Yasuko Nakagawa ,  Yoshimasa Sakamoto

IPC: C12Q1/68 ,  C12N5/08

CPC classification number: C12Q1/6883 ,  C12Q2600/158

Abstract: In neuron transplantation therapy, in terms of safety, it is preferable to use a cell population consisting only of a desired type of cells, and to use postmitotic neurons in consideration to avoid the risk of tumorigenesis. Moreover, greater therapeutic effects would be expected through the use of earlier progenitor cells in consideration of post-transplantation viability, proper network formation ability, and such. According to the present invention, Lrp4, a gene that is specifically expressed in dopaminergic neuron proliferative progenitor cells prior to cell cycle exit, was identified. The use of Lrp4 expression in cells as an index allows for the isolation. of cells suitable for transplantation therapy of neurodegenerative diseases such as Parkinson's disease in terms of safety, survival rate, and network formation ability.

Abstract translation: 在神经元移植治疗中，在安全性方面，优选使用仅由期望类型的细胞组成的细胞群，并考虑使用神经元神经元以避免肿瘤发生的风险。 此外，考虑到移植后的生存力，适当的网络形成能力等，通过使用较早的祖细胞可以预期更大的治疗效果。 根据本发明，鉴定了在细胞周期退出之前在多巴胺能神经元增殖祖细胞中特异性表达的基因Lrp4。 在细胞中使用Lrp4表达作为指标，可以进行分离。 的细胞在安全性，存活率和网络形成能力方面适用于帕金森病等神经变性疾病的移植治疗。