The invention discloses a preparation method for a lactobacillus plantarum viable bacterial preparation. The method includes: culturing lactobacillus plantarum to a stable phase, adding Saccharomyces cerevisiae subjected to wall-breaking treatment, centrifuging the fermented liquid to obtain bacterial sludge, blending a protective agent into the bacterial sludge, conducting vacuum drying, and mixing the lactobacillus plantarum powder obtained by vacuum drying with oligosaccharide. The method provided by the invention is simple and low in cost, realizes reclamation of wastes and is suitable for industrial production.