The invention discloses a method for preparing a green feed-use antibacterial growth promoting agent through high temperature fermentation by means of aquatic protein and Chinese herbal medicine. The method comprises the following main steps that fresh low-value aquatic protein is minced for use or aquatic product processing waste water is steamed and concentrated till the protein content ranges from 15% to 20% for use; the Chinese herbal medicine is smashed and sieved through a 60-mesh sieve; the minced low-value aquatic protein or concentrated aquatic product processing waste water is fixed with the smashed Chinese herbal medicine, and nutrients and water are added into the mixture, the materials are mixed to be uniform and serve as a fermentation medium; high-temperature-resistant Bacillus licheniformis HL-3 is inoculated on the fermentation medium for solid aerobic fermentation; a fermented product is subjected to drying, smashing and packaging, and a feed additive finished product is prepared. By means of the method for preparing the green feed-use antibacterial growth promoting agent through high temperature fermentation by means of the aquatic protein and Chinese herbal medicine, the green feed-use antibacterial growth promoting agent which is rich in ingredients of small peptide, Chinese herbal medicine effective ingredients, high-temperature-resistant protease, high-temperature-resistant non-starch polysaccharase and probiotics can be prepared, the good effects of promoting growth and resisting diseases are achieved, the growth promoting agent can completely replace feed-use antibiotics and has a good application prospect and social significance.