A method and system for interaction analysis are disclosed. An example method for evaluation of an interaction between an analyte in a fluid sample and a ligand immobilized on a sensor surface of a biosensor includes providing a reference binding curve, representing a reference interaction for a predetermined acquisition cycle, by acquiring, using the biosensor, one or more binding curves for a reference-analyte ligand interaction at the predetermined acquisition conditions, acquiring, using the biosensor, a sample binding curve for the analyte ligand interaction for the predetermined acquisition cycle including at least one association phase wherein the sensor surface is put into contact with a fluid sample including analyte at a predetermined concentration, and generating a graphical user interface, including an upper threshold curve and a lower threshold curve defined with respect to the reference binding curve.