The invention discloses an accurate positioning and temporary locking system for a guide pipe frame base. The accurate positioning and temporary locking system comprises a plane measuring system, a plurality of plane adjusting hydraulic oil cylinders, a plurality of vertical adjusting hydraulic oil cylinders and an adjusting control system. The plane measuring system measures the levelness of a top flange face of a guide pipe frame. The multiple plane adjusting hydraulic oil cylinders are detachably arranged on guide pipe frame landing legs, are horizontally distributed in the circumferential direction of the guide pipe frame landing legs and correspond to the outer side walls of the steel pipe piles matched with the guide pipe frame landing legs. The multiple vertical adjusting hydraulic oil cylinders are detachably arranged on the guide pipe frame landing legs, vertically distributed in the circumferential direction of the guide pipe frame landing legs and correspond to the top ends of the steel pipe piles matched with the guide pipe frame landing legs. The adjusting control system is connected with the multiple plane adjusting hydraulic oil cylinders and the multiple vertical adjusting hydraulic oil cylinders in a control manner and is in data connection with the plane measuring system. According to the system, the guide pipe frame can be subjected to accurate plane positioning; in addition, the guide pipe frame can be temporarily locked, plane positioning precision and reliability are further guaranteed, and accordingly the construction requirement can be met.