The invention discloses a method for producing 5-HMF (5-hydroxymethyl-furfural). The method comprises steps as follows: a PDA (potato dextrose agar) solid slant culture medium is inoculated with aspergillus niger xj strains, the aspergillus niger xj strains are cultured for 3-5 days at the temperature of 27 DEG C and activated 2-3 times, the activated strains are inoculated to a 10L fermentation tank accommodating a PDA liquid seed culture medium and are subjected to small-tank culture for 2-3 d under the conditions that the temperature is 26-28 DEG C, the speed is 100-120 rpm and ventilation volume is controlled to be 500-700 L/h, and a seed solution is obtained; 10%-15% (v/v) of the seed solution is inoculated to a 80L fermentation tank accommodating a PDA fermentation culture medium and subjected to large-tank culture for 3 d under the conditions that the temperature is 26-28 DEG C, the stirring speed is 80 rpm and the ventilation volume is controlled to be 2.5m<3>/h, then fermentation is stopped, fermentation broth is removed through filtering with four layers of sterile gauze, mycelia are collected, and 5-HMF is obtained. According to the method, the content of obtained 5-HMF is high, the culture media are easily available, the fermentation conditions are stable, and the process is simple.