中科微点-全球专利检索

  1. Login
  2. Sign Up
Invention Publication
CN107112193A 用于确定大分子组装体的结构的方法 有权

用于确定大分子组装体的结构的方法
Abstract:
一种确定大分子组装体(MMA)的结构的方法，其包括以下步骤：(a)产生待研究的MMA物质的前驱体离子；(b)将所述MMA前驱体离子输送到碎裂区；(c)在所述碎裂区中进行所述MMA前驱体离子的脉冲碎裂；(d)对于第一多个MMA前驱体离子，检测所述所得MMA碎片离子的空间分布和所述MMA碎片离子的m/z分布两者；(e)分析由待研究的所述MMA物质的所述第一多个前驱体离子形成的碎片离子的所述空间和m/z分布，以确定那些碎片离子在所述前驱体MMA的所述结构内的相对位置；以及(f)根据对碎片离子的所述空间和m/z分布的所述分析重建所述MMA的三维(3D)结构。
Abstract(English):

A method of determining the structure of a macromolecular assembly (MMA) comprises the steps of (a) generating precursor ions of an MMA species to be investigated; (b) transporting the MMA precursor ions to a fragmentation zone; (c) carrying out pulsed fragmentation of the MMA precursor ions in the fragmentation zone; (d) for a first plurality of MMA precursor ions, detecting both a spatial distribution of the resultant MMA fragment ions, and an m/z distribution of the MMA fragment ions; (e) analyzing the spatial and m/z distributions of fragment ions formed from the said first plurality of precursor ions of the MMA species to be investigated, to determine the relative positions of those fragment ions within the structure of the precursor MMA; and (f) reconstructing the three dimensional (3D) structure of the MMA from the analysis of the spatial and m/z distributions of fragment ions.

Public/Granted literature
Patent Agency Ranking
0/0