The invention relates to a transmission electron microscope in-situ nanomechanical tensile testing sample bonding method, which uses a developed micromechanical unit to transfer a sample under an optical microscope and uses epoxy resin to fix the sample. A counterweight, cantilevers and operating tools are fixedly bonded by using epoxy resin, and a three-coordinate micromotion platform of the optical microscope is adopted as a moving unit; the ultrasonically dispersed sample is transferred into a spray unit, and a spraying method is adopted to uniformly spray the sample onto a copper net; the micromechanical unit is used for transferring a single sample onto a drawing unit of an in-situ nanomechanical testing system under the optical microscope; the second-stage operating tool of the micromechanical unit dips epoxy resin and transfer the epoxy resin to the bonded part of the sample, and the sample bonded by the epoxy resin is kept still under room temperature for 24 hours for solidification. The transmission electron microscope in-situ nanomechanical tensile testing sample bonding method provided by the invention is simple, low-cost and nondestructive, and a transmission electron microscope in-situ nanomechanical tensile testing method for one-dimensional nanomaterials is achieved.