Disclosed is a manufacturing method for a USB (universal serial bus) interface. The manufacturing method comprises the steps of forming a first trench in a first signal transmission line of the USB interface, wherein the first signal transmission line is divided into a first connection section and a second connection section through the first trench; forming a second trench in a second signal transmission line of the USB interface, wherein the second signal transmission line is divided into a third connection section and a fourth connection section through the second trench; setting USB test switch tubes on the first signal transmission line and the second signal transmission line; performing signal transmission test on the USB interface through the USB test switch tubes; when the USB interface passes the signal transmission test, removing the USB test switch tubes; and connecting the first connection section and the second connection section through a first resistor, and connecting the third connection section and the fourth connection section through a second resistor. In addition, the invention also provides the USB interface and a mobile terminal, wherein the USB interface has a relatively small volume.