The invention relates to the field of rubber materials and rubber processing application, and discloses a rubber composition and vulcanized rubber and a preparation method and application thereof. Thecomposition comprises main rubber, a reinforcing agent, an antioxidant, a vulcanizing agent, a vulcanization accelerator and an activator, and optionally, comprises a softening agent and/or a coupling agent, wherein the reinforcing agent is a reinforcing agent mixture containing carbon black A and carbon black B, the carbon black A has an iodine absorption value of 70 to 180 g/kg, and nitrogen adsorption specific surface area of 70 to 145 m<2> /g; the carbon black B has iodine adsorption value of 20 to 50 g/kg, and nitrogen adsorption specific surface area of 25 to 40 m<2> /g. The rubber composition provided by the present invention can prepare the vulcanized rubber having excellent mechanical properties, low heat generation, low rolling resistance, good wet skid resistance and excellentwear resistance.