The invention discloses a preparation method and an operation and control method for a magnetic spiral moving microrobot. The method comprises the steps that a spiral seaweed biological template the same as the magnetic spiral moving microrobot needed by a user in structure parameter is selected; fixing and intensifying are carried out; colloidal-state palladium excitation is carried out; dispergation is carried out; chemical nickel-plating is carried out; and washing and drying are carried out. A system comprises a nested coil mechanism, a positive and negative binding post, a container platform, a driver, a digital quantity and analog quantity conversion device, an upper computer and an observation and recording device; and the nested coil mechanism is formed by assembling three pairs ofHelmholtz coils in an orthogonal nesting manner, the upper computer is connected with the positive and negative binding post through the digital quantity and analog quantity conversion device and thedriver, the container platform is located in the center of the nested coil mechanism, and the observation and recording device is installed above the nested coil mechanism. By means of the method andsystem, controlled and batched preparation of the magnetic spiral moving microrobot with different structure parameters can be achieved, and accurate operation and control of the magnetic spiral moving microrobot are achieved.