The invention provides a water-resistant enhanced wound healing film and a preparation method thereof. The wound healing film comprises the following raw materials: 5-10 mg/L of ultrafine eggshell powder particles; 50-500 mg/L of ultrafine shell membrane powder particles or shell membrane soluble protein; 5-30mg/L of lysozyme; 5-10 mg/L of chitosan; 0.05-5 mg/L of glycerol; and 0.1-5 mg/L of polymer polysaccharide. The invention also provides a preparation method of the above water-resistant enhanced wound healing film. All the preparation materials are non-toxic materials with good biocompatibility. The raw materials include eggshell, shell membrane and soluble mixed protein thereof, and lysozyme in residual egg white. All components of eggshell wastes can be well-utilized. The prepared wound healing film has good water resistance, mechanical properties, antibacterial activity, protein absorption capacity and low degradation rate. The method of the invention has the advantages of simple process, strong practicability and extremely low production cost, is beneficial to cyclic utilization of resources, and is low in energy consumption.