The invention discloses a turbidity monitoring method of an urban river ecological intelligent monitoring mother station. The method comprises the following steps that: 1, an urban river ecological intelligent monitoring mother station is put into ultrapure water, a sound wave generator and a light source are started, original alignment optical detection signals OPA1 are formed on alignment CCD sensors, and original optical detection signals OPA2, OPA3, OPA3 and OPA4 are formed by four adjacent CCD sensors; 2, the urban river ecological intelligent monitoring mother is put into a to-be-detected water body to form alignment original optical detection signals OP1 on alignment CCD sensors, and form original optical detection signals OP2, OP3, OP3 and OP4 by four adjacent CCD sensors; and 3, the expression of the turbidity Z is shown in the specification. According to the turbidity monitoring method of an urban river ecological intelligent monitoring mother station, direct signals and a plurality of diffuse reflection signals of light are collected at the same time, so that the measurement precision can be improved, the structure is simplified, and the equipment cost is reduced.