A method for determining antifouling ability of a material surface is provided. The method includes (a) providing a determining device. The determining device includes a probe and a determining unit with a spring characteristic structure. The probe includes a micro particle or a micro particle and a pollutant fixed on a surface of the micro particle. The probe is fixed at one end of the spring characteristic structure. After the step (a), the method further includes (b) contacting the probe with a material surface-to-be-determined, (c) deforming the spring characteristic structure until the probe departs from the material surface-to-be-determined to recover the spring characteristic structure, and determining the level of the deformation, (d) determining the adhesion value of the probe to the material surface-to-be-determined using the deformation and (e) determining the antifouling ability of the material surface.