Invention Grant
- Patent Title: Method and kit for quantifying cardiolipin
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Application No.: US15301133Application Date: 2015-03-17
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Publication No.: US10174357B2Publication Date: 2019-01-08
- Inventor: Shin-ya Morita
- Applicant: SHIGA UNIVERSITY OF MEDICAL SCIENCE
- Applicant Address: JP Otsu-shi, Shiga
- Assignee: SHIGA UNIVERSITY OF MEDICAL SCIENCE
- Current Assignee: SHIGA UNIVERSITY OF MEDICAL SCIENCE
- Current Assignee Address: JP Otsu-shi, Shiga
- Agency: MH2 Technology Law Group, LLP
- Priority: JP2014-077076 20140403
- International Application: PCT/JP2015/057870 WO 20150317
- International Announcement: WO2015/151801 WO 20151008
- Main IPC: C12Q1/28
- IPC: C12Q1/28 ; C12Q1/26 ; C12Q1/32 ; C12Q1/44 ; C12Q1/48 ; G01N21/64 ; G01N33/92
Abstract:
A method for quantifying cardiolipin in a sample, comprises the steps of: (1) treating the sample with phospholipase D, glycerol kinase, glycerol-3-phosphate oxidase, and peroxidase and (2) measuring the fluorescence intensity, absorbance, or luminescence intensity of a compound generated in step (1) to quantify cardiolipin using a calibration curve obtained beforehand; and a kit for quantifying cardiolipin comprises phospholipase D, glycerol kinase, glycerol-3-phosphate oxidase, and peroxidase.
Public/Granted literature
- US20170114387A1 METHOD AND KIT FOR QUANTIFYING CARDIOLIPIN Public/Granted day:2017-04-27
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