A memory storage apparatus including a plurality of word lines, a plurality of bit lines, a memory cell array, and a memory controller is provided. The memory cell array includes a plurality of memory cells. The memory cells are configured to store data. Each of the memory cells is coupled to the corresponding word line and the corresponding bit line. The memory controller is configured to perform a read operation to the memory cell array. The memory controller performs a pre-charge operation to part or all of the bit lines when the memory controller enables the word lines. In addition, an operating method of a memory storage apparatus is also provided.