The present invention relates to a process and a culture medium for biofuel and biochemical production by fermentation of lignocellulose biomass. The process describes the use of a solid-liquid mixed blend formed by solid fiber pulp, hydrolysate, and a polypeptide complex. The solid fiber pulp is partially degraded by the polypeptide complex allowing microbe immobilization and, at the same time, releasing substances that affect Clostridium quorum sensing pathways. The present process and culture medium allow the improvement of biofuels and biochemical production, as butanol and acetone in an industrial scale.